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anti-Mouse (Murine) CD28 antibody for Intracellular Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Mouse (Murine)
432
370
104
71
43
33
26
26
23
23
12
6
1
1
1
1
Host
Mouse
493
200
79
71
25
23
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to Alexa Fluor 488
117
97
76
38
17
17
15
14
13
12
11
10
9
9
9
9
9
8
8
7
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
734
271
176
131
119
117
114
113
84
63
24
12
8
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2657375
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008733104 ABIN2659481 FACS ICFC PE-Cy7 Mouse IgG2b kappa Log in to see E18 3
0.0008733104 ABIN2658577 ICFC APC Hamster IgG Log in to see 37-51 9
0.0008733104 ABIN2658582 ICFC APC Mouse IgG2b kappa Log in to see E18 3
0.0008733104 ABIN2658952 ICFC PE-Cy5 Hamster IgG Log in to see 37-51 9
0.0008733104 ABIN2665643 ICFC Mouse IgG2b kappa Log in to see E18 3
0.0008733104 ABIN2658583 ICFC APC Mouse IgG2b kappa Log in to see E18 3
0.0008733104 ABIN2658578 ICFC APC Hamster IgG Log in to see 37-51 9
0.0008733104 ABIN2665642 ICFC Mouse IgG2b kappa Log in to see E18 3

Top referenced anti-CD28 antibody for Intracellular Flow Cytometry

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 22 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 293 Antibodies
Functional Studies (Func) 25 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 88 Antibodies
In vitro Assay (in vitro) 5 Antibodies

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(432), (370), (104), (71), (43), (33), (26), (26), (23), (23), (12), (6), (1), (1), (1), (1)
Host Mouse
(493), (200), (79), (71), (25), (23), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to Alexa Fluor 488
(117), (97), (76), (38), (17), (17), (15), (14), (13), (12), (11), (10), (9), (9), (9), (9), (9), (8), (8), (7), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(734), (271), (176), (131), (119), (117), (114), (113), (84), (63), (24), (12), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2657375) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone E18
Isotype IgG2b kappa

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN2657375) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN2657375) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN2657375) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD28 Antibody (ABIN2657375)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Dennehy, Elias, Zeder-Lutz, Ding, Altschuh, Lühder, Hünig: "Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5725-9, 2006

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2657375) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (Alexa Fluor 488) (ABIN2657375) anti-CD28 (CD28) antibody (Alexa Fluor 488)