anti-Mouse (Murine) CD28 antibody for Intracellular Flow Cytometry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Mouse (Murine)
496
421
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Hamster
527
202
92
77
40
30
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to APC
113
95
74
37
22
21
20
20
18
18
17
13
13
13
13
12
12
12
8
8
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Intracellular Flow Cytometry (ICFC)
795
211
185
160
143
142
133
130
89
77
26
21
12
9
8
6
6
3
3
3
3
2
2
2
1
1
1
1
1
1
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Available images

Catalog No. ABIN2658577
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658952 ICFC PE-Cy5 Hamster IgG Log in to see 37-51 9
1 ABIN2657375 FACS ICFC Alexa Fluor 488 Mouse IgG2b kappa Log in to see E18 3
1 ABIN2659481 FACS ICFC PE-Cy7 Mouse IgG2b kappa Log in to see E18 3
1 ABIN2658582 ICFC APC Mouse IgG2b kappa Log in to see E18 3
1 ABIN2665643 ICFC Mouse IgG2b kappa Log in to see E18 3
1 ABIN2658583 ICFC APC Mouse IgG2b kappa Log in to see E18 3
1 ABIN2658578 ICFC APC Hamster IgG Log in to see 37-51 9
1 ABIN2665642 ICFC Mouse IgG2b kappa Log in to see E18 3

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(496), (421), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Hamster
(527), (202), (92), (77), (40), (30), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to APC
(113), (95), (74), (37), (22), (21), (20), (20), (18), (18), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Intracellular Flow Cytometry (ICFC)
(795), (211), (185), (160), (143), (142), (133), (130), (89), (77), (26), (21), (12), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN2658577) Images
Purification The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone 37-51
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN2658577) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN2658577) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN2658577) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD28 antibody (ABIN2658577)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Pua, Dzhagalov, Chuck, Mizushima, He: "A critical role for the autophagy gene Atg5 in T cell survival and proliferation." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 25-31, 2007

Wang, Huang, Goetzl: "Type 1 sphingosine 1-phosphate G protein-coupled receptor (S1P1) mediation of enhanced IL-4 generation by CD4 T cells from S1P1 transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4885-90, 2007

Perchonock, Fernando, Quinn, Nguyen, Sun, Shapiro, Shapiro: "Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX." in: Molecular and cellular biology, Vol. 26, Issue 16, pp. 6005-15, 2006

Terrazas, Montero, Terrazas, Reyes, Rodríguez-Sosa: "Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis." in: International journal for parasitology, Vol. 35, Issue 13, pp. 1349-58, 2005

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN2658577) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 (CD28) antibody (APC) (ABIN2658577) anti-CD28 (CD28) antibody (APC)
Flow Cytometry (FACS) image for anti-CD28 (CD28) antibody (APC) (ABIN2658577) anti-CD28 (CD28) antibody (APC) (Image 2)