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anti-Mouse (Murine) CD28 antibody for Immunohistochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Mouse (Murine)
313
307
102
38
26
12
6
5
5
2
2
2
1
1
1
1
Host
Hamster
360
188
77
42
25
20
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to PerCP-Cy5.5
106
90
66
41
10
7
7
7
7
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Activation (Act), Flow Cytometry (FACS), Immunohistochemistry (IHC)
540
174
114
95
92
43
40
34
27
22
13
8
4
3
3
3
3
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2660334
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011435054 ABIN2665972 FACS IHC Hamster IgG Log in to see 37-51 9
0.0011435054 ABIN2665489 IF IP IHC WB Hamster IgG Log in to see 37-51 9
0.0011435054 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011435054 ABIN2660335 Act FACS IHC PerCP-Cy5.5 Hamster IgG Log in to see 37-51 9
0.0011435054 ABIN2665973 FACS IHC Hamster IgG Log in to see 37-51 9
0.0011435054 ABIN2665488 IF IP IHC WB Hamster IgG Log in to see 37-51 9
0.0011435054 ABIN280303 Func FACS IP IHC Syrian Hamster IgG Log in to see 37-51
0.0011435054 ABIN1889575 FACS IHC Hamster IgG AA 21-149 Log in to see AP-MAB0711
0.0011435054 ABIN2224792 FACS IP IHC Hamster IgG Log in to see 37-51
0.0011435054 ABIN440723 Func ICC FACS IHC (fro) IF IP IHC Golden Syrian Hamster IgG Log in to see 37-51
0.0011435054 ABIN2136359 FACS IP IHC Allophycocyanin Hamster IgG Log in to see 37-51
0.0011435054 ABIN3037033 Func ICC FACS IF IP IHC Hamster IgG Log in to see
0.0011435054 ABIN1525631 IHC Syrian Hamster IgG Log in to see
0.0011435054 ABIN1853951 FACS IP IHC Biotin Hamster IgG Log in to see

Top referenced anti-CD28 antibody for Immunohistochemistry

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 22 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 238 Antibodies
Functional Studies (Func) 27 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 91 Antibodies
In vitro Assay (in vitro) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 9 Antibodies

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(313), (307), (102), (38), (26), (12), (6), (5), (5), (2), (2), (2), (1), (1), (1), (1)
Host Hamster
(360), (188), (77), (42), (25), (20), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to PerCP-Cy5.5
(106), (90), (66), (41), (10), (7), (7), (7), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Activation (Act), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(540), (174), (114), (95), (92), (43), (40), (34), (27), (22), (13), (8), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2660334) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Clone 37-51
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN2660334) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN2660334) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN2660334) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD28 Antibody (ABIN2660334)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Pua, Dzhagalov, Chuck, Mizushima, He: "A critical role for the autophagy gene Atg5 in T cell survival and proliferation." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 25-31, 2007

Wang, Huang, Goetzl: "Type 1 sphingosine 1-phosphate G protein-coupled receptor (S1P1) mediation of enhanced IL-4 generation by CD4 T cells from S1P1 transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4885-90, 2007

Perchonock, Fernando, Quinn, Nguyen, Sun, Shapiro, Shapiro: "Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX." in: Molecular and cellular biology, Vol. 26, Issue 16, pp. 6005-15, 2006

Terrazas, Montero, Terrazas, Reyes, Rodríguez-Sosa: "Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis." in: International journal for parasitology, Vol. 35, Issue 13, pp. 1349-58, 2005

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN2660334) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (PerCP-Cy5.5) (ABIN2660334) anti-CD28 (CD28) antibody (PerCP-Cy5.5)
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28)  (PerCP-Cy5.5) (ABIN2660334) anti-CD28 (CD28) antibody (PerCP-Cy5.5) (Image 2)