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anti-Non-Human Primate CD28 antibody for Western Blotting

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Human, Non-Human Primate
468
410
111
72
50
34
27
27
25
24
15
5
2
2
2
2
1
1
1
Host
Mouse
522
214
86
72
40
28
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
120
99
78
38
21
20
17
16
16
16
14
12
12
12
12
11
10
10
10
7
6
6
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
783
214
185
145
140
136
128
127
90
71
26
13
13
9
8
5
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN638435
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
1 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
1 ABIN457388 FACS Func ICC IHC (f) IP WB PerCP Mouse IgG1 Log in to see CD28-2 6

Top referenced anti-CD28 antibody for Western Blotting

Similar anti-CD28 Antibodies

Application / Reactivity Non-Human Primate Human
Flow Cytometry (FACS) 6 Antibodies 338 Antibodies
Functional Studies (Func) 6 Antibodies 47 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 108 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 6 Antibodies
Activation (Act) 1 Antibodies
Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 124 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (IF) 127 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human, Non-Human Primate
(468), (410), (111), (72), (50), (34), (27), (27), (25), (24), (15), (5), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(522), (214), (86), (72), (40), (28), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(120), (99), (78), (38), (21), (20), (17), (16), (16), (16), (14), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(783), (214), (185), (145), (140), (136), (128), (127), (90), (71), (26), (13), (13), (9), (8), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN638435) Images
Specificity The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
HLDA V., WS Code 5T CD28.05
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN638435) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Gene ID 940
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN638435) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN638435) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD28 Antibody (ABIN638435)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004

Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999

Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993

Background publications

Nunès, Klasen, Ragueneau, Pavon, Couez, Mawas, Bagnasco, Olive: "CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events." in: International immunology, Vol. 5, Issue 3, pp. 311-5, 1993

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN638435) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN638435) Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) p...