anti-Primate CD28 antibody for Immunofluorescence

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Human, Primate
495
410
103
70
66
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Mouse
526
199
92
70
40
29
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
112
96
74
37
21
20
19
19
18
17
17
13
13
13
13
12
12
12
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
779
210
184
159
143
140
133
129
88
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN400890
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291313 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 405 Mouse IgG1 Log in to see CD28-2
1 ABIN4291314 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 488 Mouse IgG1 Log in to see CD28-2
1 ABIN4291315 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 647 Mouse IgG1 Log in to see CD28-2
1 ABIN4291316 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 700 Mouse IgG1 Log in to see CD28-2
1 ABIN4291318 ICC IF IHC IHC (fro) WB Biotin Mouse IgG1 Log in to see CD28-2
1 ABIN4291319 FACS ICC IF IHC IHC (fro) WB DyLight 650 Mouse IgG1 Log in to see CD28-2
1 ABIN4291320 FACS ICC IF IHC IHC (fro) WB DyLight 680 Mouse IgG1 Log in to see CD28-2
1 ABIN4291321 FACS ICC IF IHC IHC (fro) WB DyLight 488 Mouse IgG1 Log in to see CD28-2
1 ABIN4291323 FACS ICC IF IHC IHC (fro) WB DyLight 755 Mouse IgG1 Log in to see CD28-2
1 ABIN4291324 FACS Func ICC IF IHC IHC (fro) IP WB PerCP Mouse IgG1 Log in to see CD28-2
1 ABIN4291326 FACS ICC IF IHC IHC (fro) WB DyLight 550 Mouse IgG1 Log in to see CD28-2
1 ABIN4291327 FACS ICC IF IHC IHC (fro) WB DyLight 350 Mouse IgG1 Log in to see CD28-2
1 ABIN4291328 FACS ICC IF IHC IHC (fro) WB DyLight 405 Mouse IgG1 Log in to see CD28-2
1 ABIN4291330 FACS IF Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291331 FACS IF Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291332 FACS IF Mouse IgG2a kappa Log in to see 204-12
1 ABIN4957806 FACS IF Mouse IgG2a kappa Log in to see 204-12

General

Antigen CD28 Antibodies
Reactivity Human, Primate
(495), (410), (103), (70), (66), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(526), (199), (92), (70), (40), (29), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(112), (96), (74), (37), (21), (20), (19), (19), (18), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(779), (210), (184), (159), (143), (140), (133), (129), (88), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Characteristics Synonyms: TP44, T-cell-specific surface glycoprotein CD28
Purification Affinity Chromatography on Protein A
Sterility 0.2 μm filtered
Immunogen DC28. 1. 3. 3 hybridoma cells
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Flow Cytometry. Immunoprecipitation. Western blot. Immunohistochemistry on frozen sections. Immunoflourescence. Functional Application. T cell costimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Azide free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilization. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.
Preservative Azide free
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 (CD28) antibody (ABIN400890) anti-CD28 (CD28) antibody