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|Application / Reactivity||Primate||Human|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies||10 Antibodies|
|Flow Cytometry (FACS)||48 Antibodies||339 Antibodies|
|Functional Studies (Func)||5 Antibodies||48 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||21 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||19 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||19 Antibodies|
|Activation (Act)||1 Antibodies|
|Agonist (Agon)||3 Antibodies|
|Biochemical Assay (BCA)||3 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Reactivity||Human, Primate Alternatives|
|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling Images
|Specificity||The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.|
|Characteristics||Synonyms: TP44, T-cell-specific surface glycoprotein CD28|
|Purification||Affinity Chromatography on Protein A|
|Sterility||0.2 μm filtered|
|Immunogen||DC28. 1. 3. 3 hybridoma cells|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Flow Cytometry. Immunoprecipitation. Western blot. Immunohistochemistry on frozen sections. Immunoflourescence. Functional Application. T cell costimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
|Buffer||Azide free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilization. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.|
|Storage||4 °C/-20 °C|
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top