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|Application / Reactivity||Rat (Rattus)|
|Flow Cytometry (FACS)||68 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||32 Antibodies|
|Intracellular Flow Cytometry (ICFC)||3 Antibodies|
|Mass Cytometry (CyTOF)||1 Antibodies|
|Western Blotting (WB)||9 Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN120439) Images
|Specificity||This antibody recognizes the cell surface antigen CD28. Clone JJ319 has been identified as a highly effective co-stimulator of T cell proliferation and IL-2 secretion by resting T cells.|
|Purification||Affinity Chromatography on Protein G.|
|Endotoxin Level||Low Endotoxin|
|Immunogen||Human PHA blasts.|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN120439) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is a type I transmembrane glycoprotein, expressed by the majority of T lymphocytes and a subset of NK cells. CD28 is an essential costimulatory receptor for T cell activation and a modulator of T-cell cytokine production. Ligands of CD28 include CD80 (B7-1), CD86 (B7-2) and P13-kinase.Synonyms: T-cell-specific surface glycoprotein CD28, TP44|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN120439) Images back to top
Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN120439) Images back to top
|Buffer||PBS, pH 7.4 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-CD28 Antibody (ABIN120439)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Tacke, Hanke, Hanke et al.: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, 1997 (PubMed).
Tacke, Clark, Dallman et al.: "Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 10, pp. 5121-7, 1995 (PubMed).