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anti-Human CD3E antibody for Functional Studies

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Epitope
Dimer gamma-epsilon, Dimer delta-epsilon
30
24
24
19
15
14
14
13
13
8
8
5
5
4
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
491
475
118
75
49
30
28
28
28
28
21
13
9
9
8
7
6
5
5
2
2
2
2
1
1
Host
Mouse
444
213
210
80
20
15
2
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
101
85
72
39
23
22
21
21
20
18
17
17
17
14
14
14
14
12
12
12
12
12
7
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
742
318
218
196
147
138
114
95
91
34
24
18
13
10
9
9
6
4
3
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN638428
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN638434 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 12
1 ABIN775704 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 9
1 ABIN489928 FACS Func IHC (f) IP APC Mouse IgG1 Log in to see UCHT1 11
1 ABIN489929 FACS Func IHC (f) IP PerCP Mouse IgG1 Log in to see UCHT1 9
1 ABIN94062 FACS Func IP PE Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
1 ABIN94060 FACS Func IP Biotin Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
1 ABIN94063 FACS Func IP APC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
1 ABIN94061 FACS Func IP FITC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
1 ABIN302049 FACS Func IP PerCP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 17
1 ABIN302021 FACS Func IHC (f) IP FITC Mouse IgG1 Log in to see UCHT1 10
1 ABIN302043 FACS Func IHC (f) IP PE Mouse IgG1 Log in to see UCHT1 10
1 ABIN4291485 FACS Func ICC IF IP Mouse IgG1 Log in to see TR66 5
1 ABIN94065 Func FACS IP Mouse IgM Chain epsilon Log in to see MEM-92 10
1 ABIN207055 Func FACS IP Mouse IgM Log in to see MEM-92

Top referenced anti-CD3E antibody for Functional Studies

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 3 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 9 Antibodies
ELISA 89 Antibodies
Flow Cytometry (FACS) 287 Antibodies
Functional Studies (Func) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 105 Antibodies
Immunofluorescence (IF) 114 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 94 Antibodies
Immunoprecipitation (IP) 51 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope Dimer gamma-epsilon, Dimer delta-epsilon
(30), (24), (24), (19), (15), (14), (14), (13), (13), (8), (8), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(491), (475), (118), (75), (49), (30), (28), (28), (28), (28), (21), (13), (9), (9), (8), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Mouse
(444), (213), (210), (80), (20), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(101), (85), (72), (39), (23), (22), (21), (21), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (12), (7), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(742), (318), (218), (196), (147), (138), (114), (95), (91), (34), (24), (18), (13), (10), (9), (9), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638428) Images
Specificity The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
HLDA IV., WS Code T 96
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-57
Isotype IgG2a

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN638428) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN638428) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN638428) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN638428)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Panyi, Bagdány, Bodnár, Vámosi, Szentesi, Jenei, Mátyus, Varga, Waldmann, Gáspar, Damjanovich: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003

Dave, Cao, Browne, Alarcon, Fernandez-Miguel, Lafaille, de la Hera, Tonegawa, Kappes: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994

Hilgert, Fran?k, Stefanová, Kaslík, Jirka, Kristofová, Rossmann, Soucek, Horejsi: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993

Soucek, Chudomel, Hrubá, Lindnerová: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, 1991

Background publications

Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004

Horejsí, Angelisová, Bazil, Kristofová, Stoyanov, Stefanová, Hausner, Vosecký, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638428) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (Dimer gamma-epsilon) (ABIN638428) Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. C...