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anti-Human CD3E antibody for Immunocytochemistry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Human
503
484
121
84
53
34
29
29
28
28
21
17
14
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Mouse
447
219
214
80
22
15
2
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
102
85
73
39
23
22
21
21
20
18
18
17
17
14
14
14
14
12
12
12
12
12
8
7
7
6
6
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
754
326
224
194
143
142
110
98
89
42
28
18
13
10
9
9
7
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4900707
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.70179 ABIN4900708 FACS ICC IP Mouse IgG1 Log in to see UCHT1 34
25.70179 ABIN4898339 FACS ICC Alexa Fluor 594 Mouse IgG1 Log in to see UCHT1
25.70179 ABIN4898340 FACS ICC Alexa Fluor 594 Mouse IgG1 Log in to see UCHT1
25.70179 ABIN4900883 FACS ICC Mouse IgG1 Log in to see UCHT1R
25.70179 ABIN4900882 FACS ICC Mouse IgG1 Log in to see UCHT1R
25.70179 ABIN4900884 FACS ICC Mouse IgG1 Log in to see UCHT1R
1 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
1 ABIN4291485 FACS Func ICC IF IP Mouse IgG1 Log in to see TR66 5
1 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
1 ABIN4291521 FACS ICC IF IP WB Rabbit Log in to see Polyclonal
1 ABIN457415 FACS ICC IHC (f) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
1 ABIN457363 FACS ICC IHC (f) IP WB PE Mouse IgG1 Activation Site Log in to see APA1-1 7
1 ABIN1858299 ICC IHC IP WB Rabbit IgG AA 28-185 Log in to see Polyclonal
1 ABIN440731 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see APA1-1
1 ABIN2618523 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
1 ABIN4291590 ICC IF IHC IHC (fro) Biotin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
1 ABIN4291597 FACS ICC IF IHC (fro) PerCP Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
1 ABIN4291599 FACS ICC IF IHC IHC (fro) DyLight 350 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
1 ABIN4291588 FACS ICC IF IHC IHC (fro) Alexa Fluor 700 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
1 ABIN4291585 FACS ICC IF IHC IHC (fro) Alexa Fluor 405 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1

Top referenced anti-CD3E antibody for Immunocytochemistry

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 3 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 9 Antibodies
ELISA 87 Antibodies
Flow Cytometry (FACS) 294 Antibodies
Functional Studies (Func) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 103 Antibodies
Immunofluorescence (IF) 110 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 98 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(503), (484), (121), (84), (53), (34), (29), (29), (28), (28), (21), (17), (14), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Mouse
(447), (219), (214), (80), (22), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(102), (85), (73), (39), (23), (22), (21), (21), (20), (18), (18), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (12), (8), (7), (7), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
(754), (326), (224), (194), (143), (142), (110), (98), (89), (42), (28), (18), (13), (10), (9), (9), (7), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN4900707) Images
Specificity Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York, Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York, Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Characteristics Clone UCHT1 was used by HLDA to establish CD designation
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Human thymocytes (1),
Clone UCHT1
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN4900707) Images back to top
Antigen
Alternative Name CD3 epsilon (CD3E Antibody Abstract)
Gene ID 916
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Hematopoietic Stem Cells, Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN4900707) Images back to top
Application Notes Flow Cytometry 0.25 μg/10^6 cells, Immunoprecipitation, T Cell Stimulation, Immunocytochemistry 8-25 μg/mL, CyTOF-reported
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN4900707) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD3E Antibody (ABIN4900707)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Moore, Cui, Warsi, Durafourt, Zorko, Owen, Antel, Bar-Or: "Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 2, pp. 761-72, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Huang, Lu, Wu, Ouyang, Chen: "T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 54, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Patsoukis, Bardhan, Chatterjee, Sari, Liu, Bell, Karoly, Freeman, Petkova, Seth, Li, Boussiotis: "PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation." in: Nature communications, Vol. 6, pp. 6692, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Zhu, Huang, Stålesen, Hansson, Li: "Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation." in: Journal of thrombosis and haemostasis : JTH, Vol. 12, Issue 7, pp. 1156-65, 2014 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).

Ruan, Thorn, Liu, Li, Yang, Zhang, Zhang: "The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma." in: International journal of oncology, Vol. 44, Issue 6, pp. 2103-10, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Sgarbanti, Marsili, Remoli, Stellacci, Mai, Rotili, Perrotti, Acchioni, Orsatti, Iraci, Ferrari, Borsetti, Hiscott, Battistini: "IκB kinase ε targets interferon regulatory factor 1 in activated T lymphocytes." in: Molecular and cellular biology, Vol. 34, Issue 6, pp. 1054-65, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Renand, Milpied, Rossignol, Bruneau, Lemonnier, Dussiot, Coulon, Hermine: "Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs." in: PLoS ONE, Vol. 8, Issue 12, pp. e85589, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Fan, Zhang, Huang, Liu, Guo, Zhang, Luo: "Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif." in: PLoS ONE, Vol. 9, Issue 9, pp. e108253, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Wang, Xu, Zhou, Lo, Lau, Nangle, Yang, Zhang, Schimmel: "Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases." in: The Journal of biological chemistry, Vol. 288, Issue 41, pp. 29223-8, 2013 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Jones, Stumhofer, Foster, Twohig, Hertzog, Topley, Williams, Hunter, Jenkins, Wang, Jones: "Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 409-19, 2011 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).

Ramgolam, Sha, Marcus, Choudhary, Troiani, Chopra, Markovic-Plese: "B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4518-26, 2011 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Pennino, Eyerich, Scarponi, Carbone, Eyerich, Nasorri, Garcovich, Traidl-Hoffmann, Albanesi, Cavani: "IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4880-8, 2010 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Eikawa, Ohue, Kitaoka, Aji, Uenaka, Oka, Nakayama: "Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 11, pp. 6734-40, 2010 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Longo, Tripiciano, Fiorelli, Bellino, Scoglio, Collacchi, Alvarez, Francavilla, Arancio, Paniccia, Lazzarin, Tambussi, Din, Visintini, Narciso, Antinori, DOffizi, Giulianelli, Carta, Di Carlo et al.: "Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up. ..." in: Vaccine, Vol. 27, Issue 25-26, pp. 3306-12, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Witte, Gruetz, Volk, Looman, Asadullah, Sterry, Sabat, Wolk et al.: "Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these ..." in: Genes and immunity, Vol. 10, Issue 8, pp. 702-14, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Hirosako, Goto, Fujii, Tsumori, Hirata, Tsumura, Kamohara, Kohrogi: "Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells." in: Clinical and experimental immunology, Vol. 155, Issue 2, pp. 266-74, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Lundqvist, Yokoyama, Smith, Berg, Childs: "Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells." in: Blood, Vol. 113, Issue 24, pp. 6120-7, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Ni, Walcheck: "Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation." in: Clinical immunology (Orlando, Fla.), Vol. 133, Issue 2, pp. 257-64, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Tran, Thacker, Louie, Oliver, White, Endres, Urquhart, Chung, Fox: "Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2989-98, 2008 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Stebbings, Findlay, Edwards, Eastwood, Bird, North, Mistry, Dilger, Liefooghe, Cludts, Fox, Tarrant, Robinson, Meager, Dolman, Thorpe, Bristow, Wadhwa, Thorpe, Poole: ""Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 5, pp. 3325-31, 2007 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN4900707) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN4900707) Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blo...
Immunocytochemistry (ICC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN4900707) CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human periphe...