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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Flow Cytometry (FACS)||329 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||119 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies|
|Epitope||Activation Site Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CD3E antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-CD3E AntibodyTarget Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN457415) Images
|Specificity||The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.|
|Purification||Purified from cell culture supernatant by protein-A affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||Purified human CD3 proteins isolated from thymus|
Target Details CD3EProduct Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN457415) Images back to top
|Alternative Name||CD3E (CD3E Antibody Abstract)|
|Background||CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells|
Application DetailsProduct Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN457415) Images back to top
Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 µg/mL )
Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 1 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.01 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/mL ) in PBS + 1 % BSA + 0.01 % saponine in dark, 20 min. RT.Wash with PBS + 1 % BSA + 0.01 % saponine, resuspend in PBS.
Immunohistochemistry (frozen sections)Immunocytochemistry
Application note: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN457415) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD3E Antibody (ABIN457415)Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
|Product cited in:||
Tailor, Tsai, Shameli et al.: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, 2008 (PubMed).
Wong, Dutly, Sacher et al.: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, 2007 (PubMed).
Rieux-Laucat, Hivroz, Lim et al.: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006 (PubMed).
Gil, Schrum, Alarcón et al.: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, 2005 (PubMed).
Torres, Alcover, Zapata et al.: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003 (PubMed).
Delgado, Fernández, Dave et al.: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, 2000 (PubMed).
Alarcón, Ley, Sánchez-Madrid et al.: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, 1991 (PubMed).