You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CD3E antibody for Immunocytochemistry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Epitope
Activation Site
31
23
19
15
14
13
13
12
8
8
6
6
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
523
412
124
90
65
45
44
43
43
43
30
26
25
24
22
22
21
7
6
2
2
2
2
1
1
Host
Mouse
359
225
211
79
35
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
97
82
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
691
297
217
168
128
91
88
85
84
34
25
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN457415
$ 254.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194078 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.0010194078 ABIN211097 ICC FACS IP WB Mouse IgG1 Log in to see
0.0010194078 ABIN2618523 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.0010194078 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.0010194078 ABIN343654 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010194078 ABIN4291600 FACS ICC IF IHC (fro) DyLight 405 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291601 FACS ICC IF IHC (fro) DyLight 405LS Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291584 FACS ICC IF IHC (fro) PE Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291585 FACS ICC IF IHC (fro) Alexa Fluor 405 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291586 FACS ICC IF IHC (fro) Alexa Fluor 488 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291587 FACS ICC IF IHC (fro) Alexa Fluor 647 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291588 FACS ICC IF IHC (fro) Alexa Fluor 700 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291589 FACS ICC IF IHC (fro) Allophycocyanin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291590 FACS ICC IF IHC (fro) Biotin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291591 FACS ICC IF IHC (fro) DyLight 650 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291592 FACS ICC IF IHC (fro) FITC Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291593 FACS ICC IF IHC (fro) DyLight 680 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291594 FACS ICC IF IHC (fro) DyLight 488 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010194078 ABIN4291596 FACS ICC IF IHC (fro) DyLight 755 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1

Top referenced anti-CD3E antibody for Immunocytochemistry

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies
Immunocytochemistry (ICC) 52 Antibodies
Immunofluorescence (IF) 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 168 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope Activation Site
(31), (23), (19), (15), (14), (13), (13), (12), (8), (8), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(523), (412), (124), (90), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (6), (2), (2), (2), (2), (1), (1)
Host Mouse
(359), (225), (211), (79), (35), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(97), (82), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(691), (297), (217), (168), (128), (91), (88), (85), (84), (34), (25), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN457415) Images
Specificity The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Purified human CD3 proteins isolated from thymus
Clone APA1-1
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN457415) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN457415) Images back to top
Application Notes Flow Cytometry:
Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 µg/mL )
Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 1 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.01 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/mL ) in PBS + 1 % BSA + 0.01 % saponine in dark, 20 min. RT.Wash with PBS + 1 % BSA + 0.01 % saponine, resuspend in PBS.
Immunohistochemistry (frozen sections)Immunocytochemistry
Application note: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN457415) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN457415)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Tailor, Tsai, Shameli, Serra, Wang, Robbins, Nagata, Szymczak-Workman, Vignali, Santamaria: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, 2008

Wong, Dutly, Sacher, Lee, Hwang, Liu, Keshavjee, Hu, Waddell: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, 2007

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006

Gil, Schrum, Alarcón, Palmer: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, 2005

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003

Delgado, Fernández, Dave, Kappes, Alarcón: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, 2000

Alarcón, Ley, Sánchez-Madrid, Blumberg, Ju, Fresno, Terhorst: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, 1991