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anti-Human CD3E antibody for Immunohistochemistry (Frozen Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human, Non-Human Primate
524
412
124
90
65
45
44
43
43
43
30
26
25
24
22
22
21
7
5
2
2
2
2
1
1
Host
Mouse
359
225
211
79
35
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
97
82
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
691
298
217
168
128
92
88
85
84
34
24
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN638434
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN489928 Func FACS IHC (fro) IP APC Mouse IgG1 Log in to see UCHT1 11
0.000883884 ABIN489929 Func FACS IHC (fro) IP PerCP Mouse IgG1 Log in to see UCHT1 9
0.000883884 ABIN775704 Func IHC (fro) FACS IP Mouse IgG1 Log in to see UCHT1 9
0.000883884 ABIN457415 ICC FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
0.000883884 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.000883884 ABIN2618523 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.000883884 ABIN3043629 IHC (fro) Mouse IgG1 Log in to see CA-3
0.000883884 ABIN2618522 IHC (fro) Mouse IgG1 Log in to see CA-3
0.000883884 ABIN229704 FACS IHC (fro) IP APC Mouse IgG1 Log in to see
0.000883884 ABIN2962185 IHC (fro) IHC (p) ELISA WB Rabbit AA 156-168 Log in to see Polyclonal
0.000883884 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.000883884 ABIN4291600 FACS ICC IF IHC (fro) DyLight 405 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291601 FACS ICC IF IHC (fro) DyLight 405LS Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291584 FACS ICC IF IHC (fro) PE Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291585 FACS ICC IF IHC (fro) Alexa Fluor 405 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291586 FACS ICC IF IHC (fro) Alexa Fluor 488 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291587 FACS ICC IF IHC (fro) Alexa Fluor 647 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291588 FACS ICC IF IHC (fro) Alexa Fluor 700 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.000883884 ABIN4291589 FACS ICC IF IHC (fro) Allophycocyanin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1

Top referenced anti-CD3E antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD3E Antibodies

Application / Reactivity Non-Human Primate Human
Flow Cytometry (FACS) 6 Antibodies 225 Antibodies
Functional Studies (Func) 4 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 57 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 86 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 85 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human, Non-Human Primate
(524), (412), (124), (90), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (5), (2), (2), (2), (2), (1), (1)
Host Mouse
(359), (225), (211), (79), (35), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(97), (82), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(691), (298), (217), (168), (128), (92), (88), (85), (84), (34), (24), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638434) Images
Specificity The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex. HLDA I, WS Code T 3HLDA III, WS Code T 126HLDA III, WS Code T 471HLDA VI, WS Code T 6T-CD3.1
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen human thymocytes followed by Sezary T cells
Clone UCHT1
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN638434) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-82 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN638434) Images back to top
Application Notes Flow Cytometry:
Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Immunohistochemistry (frozen sections)
Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Functional Application: The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN638434) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN638434)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Siegers, Swamy, Fernández-Malavé, Minguet, Rathmann, Guardo, Pérez-Flores, Regueiro, Alarcón, Fisch, Schamel: "Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2537-44, 2007

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998

Fisch, Malkovsky, Braakman, Sturm, Bolhuis, Prieve, Sosman, Lam, Sondel: "Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis." in: The Journal of experimental medicine, Vol. 171, Issue 5, pp. 1567-79, 1990

Garson, Beverley, Coakham, Harper: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." in: Nature, Vol. 298, Issue 5872, pp. 375-7, 1982

Background publications

Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006

Lin, Liu, Chen, Wang, Medeiros, Hsu: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638434) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN638434) Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific ...