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anti-Human CD3E antibody for Immunohistochemistry (Frozen Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Epitope
AA 23-207
30
23
19
15
14
13
13
12
8
7
6
6
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
505
416
107
71
48
28
27
26
26
26
21
13
9
8
7
7
6
5
5
2
2
2
2
1
1
Host
Rabbit
384
213
204
79
34
21
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
97
83
69
40
21
20
20
19
17
17
17
15
15
13
12
12
12
12
12
10
10
10
7
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
700
278
212
165
109
90
84
81
78
34
32
10
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3043805
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088272867 ABIN2618523 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.00088272867 ABIN3043629 IHC (fro) Mouse IgG1 Log in to see CA-3
0.00088272867 ABIN229704 FACS IHC (fro) IP APC Mouse IgG1 Log in to see
0.00088272867 ABIN2618522 IHC (fro) Mouse IgG1 Log in to see CA-3
0.00088272867 ABIN2962185 IHC (fro) IHC (p) ELISA WB Rabbit AA 156-168 Log in to see Polyclonal
0.00088272867 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.00088272867 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291584 FACS ICC IF IHC (fro) PE Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291588 FACS ICC IF IHC (fro) Alexa Fluor 700 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291589 FACS ICC IF IHC (fro) Allophycocyanin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291587 FACS ICC IF IHC (fro) Alexa Fluor 647 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291590 FACS ICC IF IHC (fro) Biotin Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291591 FACS ICC IF IHC (fro) DyLight 650 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291592 FACS ICC IF IHC (fro) FITC Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291594 FACS ICC IF IHC (fro) DyLight 488 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291598 FACS ICC IF IHC (fro) DyLight 550 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291601 FACS ICC IF IHC (fro) DyLight 405LS Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291593 FACS ICC IF IHC (fro) DyLight 680 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291597 FACS ICC IF IHC (fro) PerCP Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.00088272867 ABIN4291596 FACS ICC IF IHC (fro) DyLight 755 Mouse IgG1 kappa non-glycosylated Log in to see UCHT1

Similar anti-CD3E Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 14 Antibodies
Flow Cytometry (FACS) 10 Antibodies 453 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 35 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 50 Antibodies
Activity Assay (AcA) 1 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 23-207
(30), (23), (19), (15), (14), (13), (13), (12), (8), (7), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(505), (416), (107), (71), (48), (28), (27), (26), (26), (26), (21), (13), (9), (8), (7), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Rabbit
(384), (213), (204), (79), (34), (21), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(97), (83), (69), (40), (21), (20), (20), (19), (17), (17), (17), (15), (15), (13), (12), (12), (12), (12), (12), (10), (10), (10), (7), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(700), (278), (212), (165), (109), (90), (84), (81), (78), (34), (32), (10), (9), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain
Purification Immunogen affinity purified.
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Gene ID 916
UniProt P07766
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-6.JPG IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-7.JPG IHC(F): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml WB : Re...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml Lane 1:...
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Rat Spleen Tissue