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anti-Human CD3E antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human
393
243
61
46
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Rabbit
204
161
155
77
33
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
460
176
175
133
68
63
53
46
34
24
20
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN2104935
$ 273.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34458184 ABIN213673 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.34458184 ABIN462010 IHC (p) Rabbit AA 156-168 Log in to see Polyclonal
0.00089488085 ABIN385171 IHC (p) Mouse IgG1, kappa Log in to see F7-2-38 1
0.00089488085 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.00089488085 ABIN1497222 IF IHC (p) WB Mouse IgG2b Log in to see 3E10
0.00089488085 ABIN1385274 IF (p) IHC (p) WB Rabbit IgG AA 75-125 Log in to see Polyclonal
0.00089488085 ABIN672921 IF (p) IHC (p) WB Rabbit IgG AA 132-170 Log in to see Polyclonal
0.00089488085 ABIN1490980 IHC (p) Rabbit IgG Log in to see Polyclonal
0.00089488085 ABIN1732859 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089488085 ABIN2618523 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.00089488085 ABIN1414557 IHC (p) WB HRP Rabbit IgG AA 75-125 Log in to see Polyclonal
0.00089488085 ABIN1396680 IHC (p) WB Biotin Rabbit IgG AA 75-125 Log in to see Polyclonal
0.00089488085 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.00089488085 ABIN672923 IHC (p) WB Biotin Rabbit IgG AA 132-170 Log in to see Polyclonal
0.00089488085 ABIN672930 IHC (p) WB HRP Rabbit IgG AA 132-170 Log in to see Polyclonal
0.00089488085 ABIN799993 IHC (p) Mouse IgG2a Log in to see PS1
0.00089488085 ABIN385865 IHC (p) Mouse IgG2a Log in to see PS1
0.00089488085 ABIN227354 IHC (p) Mouse IgG1 Log in to see
0.00089488085 ABIN1020036 IHC (p) Rabbit IgG AA 156-168 Log in to see Polyclonal
0.00089488085 ABIN1020035 IHC (p) Rabbit IgG AA 156-168 Log in to see Polyclonal

Top referenced anti-CD3E antibody for Immunohistochemistry (Paraffin-embedded Sections)

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Application / Reactivity Human
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
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Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 32 Antibodies
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General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(393), (243), (61), (46), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(204), (161), (155), (77), (33), (21), (1)
Clonality
Monoclonal
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(460), (176), (175), (133), (68), (63), (53), (46), (34), (24), (20), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2104935) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2104935) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Image 2)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2104935) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Image 3)
'Independent Validation' Badge
Lot Number HB08JA0310
Method validated Immunohistochemistry
Positive Control Human lymph node tissue
Negative Control Human liver tissue
Notes Light signal was detected in positive control sample but not negative control samples.
Primary Antibody
  • Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)
  • Catalog number: ABIN2104935
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: HB08JA0310
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Human lymph node tissue stained with antibody
  • Negative control: Human liver tissue stained with antibody
  • Isotype control: Human lymph node tissue stained with isotype control
  • Secondary only control: Human lymph node tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. Discovery instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 5.0 ug/ml in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes No experimental challenges noted.
Validation Images
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 1. Micrograph image of positive control (human lymph node tissue) stained with...
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 2. Micrograph image of positive control (human lymph node tissue) stained with...
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 3. Micrograph image of positive control (human lymph node tissue) stained with...
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 4. Micrograph image of negative control (human liver tissue) stained with anti...
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 5. Micrograph image of negative control (human liver tissue) stained with seco...
Immunohistochemistry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN2104935) Figure 6. Micrograph image of negative control (human liver tissue) stained with rabb...