anti-Mouse (Murine) CD3E antibody for ELISA

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Mouse (Murine)
482
460
121
87
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Hamster
437
225
187
58
22
15
3
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to FITC
88
75
73
32
24
22
21
20
20
18
17
16
14
14
14
14
14
12
12
12
12
10
8
8
7
7
6
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
696
324
226
200
143
140
111
97
93
42
29
18
13
9
9
7
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2739339
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2739336 FACS IP ELISA WB PE Hamster IgG Log in to see 145-2C11
1 ABIN2739338 FACS IP ELISA WB APC Hamster IgG Log in to see 145-2C11
1 ABIN2962185 IHC (fro) IHC (p) ELISA WB Rabbit AA 156-168 Log in to see Polyclonal
1 ABIN2739337 FACS IP ELISA WB Biotin Hamster IgG Log in to see 145-2C11
1 ABIN2739340 FACS IP ELISA WB Hamster IgG Log in to see 145-2C11
1 ABIN2749613 ELISA IHC IP WB FITC Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN2749614 ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2749615 ELISA IHC IP WB FITC Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2749612 IP IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2746375 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2746376 ELISA IHC IP WB Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN2749611 ELISA IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2749610 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5558239 ELISA IHC IP WB Biotin Rabbit C-Term Log in to see Polyclonal
1 ABIN5558237 ELISA WB Biotin Rabbit Middle Region Log in to see Polyclonal

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
Depletion (Dep) 3 Antibodies
ELISA 16 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 385 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 11 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 105 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 40 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(482), (460), (121), (87), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Hamster
(437), (225), (187), (58), (22), (15), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to FITC
(88), (75), (73), (32), (24), (22), (21), (20), (20), (18), (17), (16), (14), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(696), (324), (226), (200), (143), (140), (111), (97), (93), (42), (29), (18), (13), (9), (9), (7), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Specificity Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
Purification Protein G purified
Immunogen Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/FITC at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles
Storage 4 °C
Storage Comment Store at 4°C, avoid freeze thaw cycles and prolonged exposure to light.