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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Flow Cytometry (FACS)||329 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||119 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD3E antibody is conjugated to PE-Cy5 Alternatives|
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Immunocytochemistry (ICC), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (IHC)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD3E AntibodyTarget Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN2658968) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.|
Target Details CD3EProduct Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN2658968) Images back to top
|Alternative Name||CD3e (CD3E Antibody Abstract)|
|Background||CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN2658968) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN2658968) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD3E Antibody (ABIN2658968)Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Kinnaert, Pradier, Bournonville et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal ..." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, 1997 (PubMed).
Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994 (PubMed).
Jacobs, Vandeputte, Tolkamp et al.: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994 (PubMed).
Salvadori, Gansbacher, Pizzimenti et al.: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994 (PubMed).
Vossen, Tibbe, Kroos et al.: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995 (PubMed).
Henrickson, Reid, Bellet et al.: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995 (PubMed).
Leo, Foo, Sachs et al.: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987 (PubMed).
Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).
Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).
ImagesProduct Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN2658968) back to top