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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Flow Cytometry (FACS)||329 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||119 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies|
|Epitope||epsilon subunit Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD3E antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD3E AntibodyTarget Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images
|Specificity||The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.|
|Purification||Purified by protein G|
|Purity||> 95 % (by SDS-PAGE)|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.|
|Immunogen||Mouse BM10-37 cytotoxic T lymphocytes|
Target Details CD3EProduct Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
|Alternative Name||CD3E (CD3E Antibody Abstract)|
|Background||CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells|
Application DetailsProduct Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
Flow Cytometry: Recommended dilution: 1-2 µg/mL (million cells)
Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg protein in 1 mL cell lysate
Functional Application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion
Working concentrations should be determined by the investigator.
CD and Related Antigens & MHC (Rodent)
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN1302512) Images back to top
|Buffer||Azide free phosphate buffered saline (PBS), approx. pH 7.4.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD3E Antibody (ABIN1302512)Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
|Product cited in:||
Han, Murray-Segal, Gershenzon et al.: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, 2000 (PubMed).
Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, 1998 (PubMed).
Jacobs, Vandeputte, Tolkamp et al.: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994 (PubMed).
Salvadori, Gansbacher, Pizzimenti et al.: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994 (PubMed).
Vossen, Tibbe, Kroos et al.: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995 (PubMed).
Henrickson, Reid, Bellet et al.: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995 (PubMed).
Leo, Foo, Sachs et al.: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987 (PubMed).
Samelson, OShea, Luong et al.: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, 1987 (PubMed).
Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).