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anti-Mouse (Murine) CD3E antibody for Immunocytochemistry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Epitope
epsilon subunit
31
15
14
13
13
11
8
8
6
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
392
244
61
46
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Hamster
203
161
156
77
33
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
459
175
175
133
68
64
52
46
34
23
19
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1302512
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010165847 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.0010165847 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.0010165847 ABIN457415 ICC FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
0.0010165847 ABIN2658968 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.0010165847 ABIN2658969 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.0010165847 ABIN490320 Func ICC FACS IHC (fro) IP Hamster IgG Log in to see 145-2C11
0.0010165847 ABIN490137 Func ICC FACS IHC (fro) IP Hamster IgG Log in to see 145-2C11
0.0010165847 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.0010165847 ABIN343654 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010165847 ABIN1112853 ICC IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0010165847 ABIN1854857 ICC IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010165847 ABIN2990265 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010165847 ABIN3030388 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.0010165847 ABIN1575402 ICC IHC WB Rabbit IgG Log in to see Polyclonal
0.0010165847 ABIN1853796 ICC FACS IF IP IHC WB PE Hamster IgG1 Log in to see
0.0010165847 ABIN1853795 ICC FACS IF IP IHC WB FITC Hamster IgG1 Log in to see

Top referenced anti-CD3E antibody for Immunocytochemistry

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 338 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 128 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope epsilon subunit
(31), (15), (14), (13), (13), (11), (8), (8), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(392), (244), (61), (46), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Hamster
(203), (161), (156), (77), (33), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(459), (175), (175), (133), (68), (64), (52), (46), (34), (23), (19), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images
Specificity The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.
Purification Purified by protein G
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Mouse BM10-37 cytotoxic T lymphocytes
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1-2 µg/mL (million cells)
Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg protein in 1 mL cell lysate
Functional Application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN1302512) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN1302512)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Han, Murray-Segal, Gershenzon, Zhang, Hodder, Pietersz, Mottram: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, 2000

Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, 1998

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Samelson, OShea, Luong, Ross, Urdahl, Klausner, Bluestone: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, 1987