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anti-Mouse (Murine) CD3E antibody for Immunocytochemistry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Epitope
epsilon subunit
30
23
19
15
15
14
14
12
8
8
6
6
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
479
421
108
74
48
28
27
26
26
26
21
13
9
8
7
7
6
5
5
2
2
2
2
1
1
Host
Hamster
384
212
211
74
19
13
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
95
81
66
39
21
20
19
19
17
17
15
15
15
12
12
12
12
12
12
11
10
10
10
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
684
279
210
166
110
91
86
82
74
38
24
10
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1302512
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001010224 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.001010224 ABIN2658968 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.001010224 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.001010224 ABIN2658969 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.001010224 ABIN457415 FACS ICC IHC (f) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
0.001010224 ABIN457363 FACS ICC IHC (f) IP WB PE Mouse IgG1 Activation Site Log in to see APA1-1 7
0.001010224 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN490320 Func ICC FACS IHC (fro) IP Hamster IgG Log in to see 145-2C11
0.001010224 ABIN490137 Func ICC FACS IHC (fro) IP Hamster IgG Log in to see 145-2C11
0.001010224 ABIN343654 ICC WB Rabbit IgG Log in to see Polyclonal
0.001010224 ABIN4291493 FACS ICC IF IHC IHC (fro) IP WB DyLight 405 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291498 FACS ICC IF IHC IHC (fro) IP WB Allophycocyanin Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291502 FACS ICC IF IHC IHC (fro) IP WB DyLight 680 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291503 FACS ICC IF IHC IHC (fro) IP WB DyLight 488 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291491 FACS ICC IF IHC IHC (fro) IP WB DyLight 350 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291496 FACS ICC IF IHC IHC (fro) IP WB PerCP Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291500 FACS ICC IF IHC IHC (fro) IP WB DyLight 755 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291508 FACS ICC IF IHC IHC (fro) IP WB PE Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291528 FACS ICC IF IHC IHC (fro) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see APA1-1
0.001010224 ABIN4291529 FACS ICC IF IHC IHC (fro) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see APA1-1

Top referenced anti-CD3E antibody for Immunocytochemistry

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 425 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 10 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 152 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope epsilon subunit
(30), (23), (19), (15), (15), (14), (14), (12), (8), (8), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(479), (421), (108), (74), (48), (28), (27), (26), (26), (26), (21), (13), (9), (8), (7), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Hamster
(384), (212), (211), (74), (19), (13), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(95), (81), (66), (39), (21), (20), (19), (19), (17), (17), (15), (15), (15), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(684), (279), (210), (166), (110), (91), (86), (82), (74), (38), (24), (10), (9), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images
Specificity The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.
Purification Purified by protein G
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Mouse BM10-37 cytotoxic T lymphocytes
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN1302512) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1-2 µg/mL (million cells)
Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg protein in 1 mL cell lysate
Functional Application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens & MHC (Rodent)

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN1302512) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN1302512)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Han, Murray-Segal, Gershenzon, Zhang, Hodder, Pietersz, Mottram: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, 2000

Kearse: "Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14152-7, 1998

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Samelson, OShea, Luong, Ross, Urdahl, Klausner, Bluestone: "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 8, pp. 2708-14, 1987