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anti-Mouse (Murine) CD3E antibody for Immunofluorescence

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human, Mouse (Murine), Rat (Rattus)
523
412
124
89
65
45
44
43
43
43
30
26
25
24
22
22
21
7
6
2
2
2
2
1
1
Host
Rabbit
360
224
211
79
35
21
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
97
82
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
692
297
218
167
127
92
88
85
85
34
25
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2404594
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010826335 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
0.0010826335 ABIN967568 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
0.0010826335 ABIN2657416 FACS IF CyTOF Alexa Fluor 488 Hamster IgG Log in to see 145-2C11 9
0.0010826335 ABIN2661400 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
0.0010826335 ABIN234587 FACS IF Syrian Hamster IgG Log in to see 500-A2
0.0010826335 ABIN2665989 BR FACS IF IHC Hamster IgG Log in to see 145-2C11 9
0.0010826335 ABIN2665492 FACS IF Hamster IgG Log in to see 145-2C11 9
0.0010826335 ABIN2661401 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
0.0010826335 ABIN600624 IHC (fro) FACS IF IP WB Armenian Hamster IgG Log in to see 145-2C11
0.0010826335 ABIN2665990 BR FACS IF IHC Hamster IgG Log in to see 145-2C11 9
0.0010826335 ABIN2665491 FACS IF Hamster IgG Log in to see 145-2C11 9
0.0010826335 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.0010826335 ABIN213124 FACS IHC (fro) IF IP WB Hamster IgG Log in to see
0.0010826335 ABIN226818 FACS IHC (fro) IF Hamster IgG Log in to see
0.0010826335 ABIN2225855 FACS IF IP IHC WB Hamster IgG Log in to see 145-2C11
0.0010826335 ABIN4291561 FACS IF Mouse IgG3 kappa Log in to see RIV9
0.0010826335 ABIN4291562 FACS IF Mouse IgG3 kappa Log in to see RIV9
0.0010826335 ABIN4291563 FACS IF Mouse IgG3 kappa Log in to see RIV9
0.0010826335 ABIN2225873 FACS IF Hamster IgG Log in to see 1-BB-655
0.0010826335 ABIN2165713 IHC (af) FACS IF IP WB Hamster IgG Log in to see

Top referenced anti-CD3E antibody for Immunofluorescence

Similar anti-CD3E Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 14 Antibodies
Flow Cytometry (FACS) 27 Antibodies 469 Antibodies
Immunocytochemistry (ICC) 25 Antibodies 52 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 68 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(523), (412), (124), (89), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (6), (2), (2), (2), (2), (1), (1)
Host Rabbit
(360), (224), (211), (79), (35), (21), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(97), (82), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(692), (297), (218), (167), (127), (92), (88), (85), (85), (34), (25), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purification Antigen affinity purification
Immunogen Recombinant protein of human CD3E
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck (5).
Synonyms: CD3ε, FLJ18683, T3E, TCRE,
Molecular Weight Observed MW: 23kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2404594) Immunofluorescence analysis of U2OS cell using CD3E antibody. Blue: DAPI for nuclear ...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2404594) Western blot analysis of extracts of Jurkat cell lines, using CD3E antibody.