anti-Mouse (Murine) CD3E antibody for Immunohistochemistry (Frozen Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 23-207
29
26
23
20
16
15
15
15
14
11
8
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
543
486
122
87
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Rabbit
494
232
203
64
22
15
4
1
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
96
81
77
39
28
24
23
20
20
20
19
17
17
14
14
14
14
12
12
12
12
10
10
9
9
8
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
759
346
246
204
155
147
119
102
99
42
30
27
13
9
9
7
6
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN3043805
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN439834 AcA FACS IHC IHC (fro) IHC (p) IP in vitro Rat IgG2b kappa Log in to see C363-29B (YCD3-1) 5
1 ABIN967568 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
1 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
1 ABIN1302512 Func ICC IHC (fro) FACS IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
1 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
1 ABIN439836 FACS IHC IHC (fro) IHC (p) IP in vitro Biotin Rat IgG2b kappa Log in to see C363-29B (YCD3-1) 5
1 ABIN439833 FACS IHC (fro) IP in vitro Cy5 Syrian Hamster IgG kappa Log in to see 500-A2
1 ABIN439835 FACS IHC IHC (fro) IHC (p) IP in vitro FITC Rat IgG2b kappa Log in to see C363-29B (YCD3-1)
1 ABIN439822 FACS IHC (fro) in vitro IP IHC WB FITC Armenian Hamster IgG kappa Log in to see 145-2C11
1 ABIN1176869 BR CyTox FM IHC (fro) FACS IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 10
1 ABIN2689216 IHC (fro) FACS Biotin Armenian Hamster IgG1, kappa Log in to see 145 7
1 ABIN2689215 BR IHC (f) IHC (fro) FACS IHC (zinc) IP WB Armenian Hamster IgG1, kappa Log in to see 145 5
1 ABIN2689214 IHC (fro) FACS IHC (p) Syrian Hamster IgG2, kappa Log in to see 500A2 1
1 ABIN474592 FACS IHC (fro) IP WB Hamster IgG Log in to see 145-2C11
1 ABIN2689213 IHC (fro) FACS IP Syrian Hamster IgG2, kappa Log in to see 500A2 5
1 ABIN2962185 IHC (fro) IHC (p) ELISA WB Rabbit AA 156-168 Log in to see Polyclonal
1 ABIN490319 Dep FACS Func ICC IHC (fro) IP FITC Hamster IgG Log in to see 145-2C11
1 ABIN490320 Dep FACS Func ICC IHC (fro) IP Armenian Hamster IgG Log in to see 145-2C11
1 ABIN440731 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see APA1-1
1 ABIN439829 FACS IHC (fro) IP in vitro Biotin Syrian Hamster IgG kappa Log in to see 500-A2

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 23-207
(29), (26), (23), (20), (16), (15), (15), (15), (14), (11), (8), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(543), (486), (122), (87), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Rabbit
(494), (232), (203), (64), (22), (15), (4), (1), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(96), (81), (77), (39), (28), (24), (23), (20), (20), (20), (19), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (10), (9), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(759), (346), (246), (204), (155), (147), (119), (102), (99), (42), (30), (27), (13), (9), (9), (7), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain
Purification Immunogen affinity purified.
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Synonyms: CD3 epsilon antibody|CD3e antibody|CD3E antibody|CD3e antigen epsilon polypeptide (TiT3 complex) antibody|CD3E antigen epsilon polypeptide antibody|CD3E antigen, epsilon subunit antibody|CD3e molecule epsilon antibody|CD3e molecule, epsilon (CD3 TCR complex) antibody|CD3e molecule, epsilon (CD3-TCR complex) antibody|CD3E_HUMAN antibody|IMD18 antibody|T cell antigen receptor complex epsilon subunit of T3 antibody|T cell surface antigen T3/Leu 4 epsilon chain antibody|T cell surface glycoprotein CD3 epsilon chain antibody|T-cell surface antigen T3/Leu-4 epsilon chain antibody|T-cell surface glycoprotein CD3 epsilon chain antibody|T3E antibody|TCRE antibody
Gene ID 916
UniProt P07766
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-6.JPG IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-7.JPG IHC(F): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml WB : Re...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml Lane 1:...
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Rat Spleen Tissue