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anti-Mouse (Murine) CD3E antibody for Immunoprecipitation

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Mouse (Murine)
477
418
107
72
48
28
27
26
26
26
21
13
9
8
7
7
6
5
5
2
2
2
2
1
1
Host
Hamster
384
212
206
74
19
13
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
95
81
66
39
21
20
19
19
17
17
15
15
15
12
12
12
12
12
12
10
10
10
7
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
672
277
210
165
110
90
85
82
74
34
25
10
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN135091
$ 258.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000890107 ABIN135085 FACS IP IHC WB Hamster IgG Log in to see 145-2C11 3
0.000890107 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
0.000890107 ABIN967568 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
0.000890107 ABIN1302512 Func ICC IHC (fro) FACS IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.000890107 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.000890107 ABIN135095 FACS IHC (p) IP Biotin Rat IgG2b Log in to see C363-29B 3
0.000890107 ABIN2658968 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN2659506 FACS IP WB PE-Cy7 Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN2659906 ELISA (Capture) FACS IP WB PerCP Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN135092 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
0.000890107 ABIN135098 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
0.000890107 ABIN439836 FACS IHC IHC (fro) IHC (p) IP in vitro Biotin Rat IgG2b kappa Log in to see C363-29B (YCD3-1) 5
0.000890107 ABIN1176869 BR CyTox FM IHC (fro) FACS IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 10
0.000890107 ABIN2689215 BR IHC (f) IHC (fro) FACS IHC (zinc) IP WB Armenian Hamster IgG1, kappa Log in to see 145 5
0.000890107 ABIN439835 FACS IHC IHC (fro) IHC (p) IP in vitro FITC Rat IgG2b kappa Log in to see C363-29B (YCD3-1)
0.000890107 ABIN2659507 FACS IP WB PE-Cy7 Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN2659907 ELISA (Capture) FACS IP WB PerCP Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN2658969 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.000890107 ABIN2689213 IHC (fro) FACS IP Syrian Hamster IgG2, kappa Log in to see 500A2 5
0.000890107 ABIN457415 FACS ICC IHC (f) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7

Top referenced anti-CD3E antibody for Immunoprecipitation

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 425 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 10 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 152 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(477), (418), (107), (72), (48), (28), (27), (26), (26), (26), (21), (13), (9), (8), (7), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Hamster
(384), (212), (206), (74), (19), (13), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(95), (81), (66), (39), (21), (20), (19), (19), (17), (17), (15), (15), (15), (12), (12), (12), (12), (12), (12), (10), (10), (10), (7), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(672), (277), (210), (165), (110), (90), (85), (82), (74), (34), (25), (10), (9), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN135091) Images
Characteristics The antibody detects: Mouse CD3 (Mr 25 kDa)
The 145-2C11 monoclonal antibody recognizes an epitope on the 25 kDa chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and nave T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145-2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death.
Endotoxin Level Low Endotoxin
Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN135091) Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN135091) Images back to top
Application Notes Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro depletion of CD3+ cells, In vitro activation of T cells

Sample Volume 1 mL
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN135091) Images back to top
Buffer Product is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage -20 °C
Storage Comment Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

References for anti-CD3E Antibody (ABIN135091)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Background publications

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN135091) back to top
Supplier Images
 image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN135091) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody