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anti-Mouse (Murine) CD3E antibody for Immunoprecipitation

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Mouse (Murine)
494
485
120
79
49
30
28
28
28
28
21
13
9
9
8
7
6
5
5
2
2
2
2
1
1
Host
Hamster
446
222
212
80
20
15
2
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
101
85
72
39
23
22
21
21
20
18
17
17
17
14
14
14
14
12
12
12
12
12
7
7
7
6
6
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
745
323
219
198
147
137
114
94
90
42
25
18
13
10
9
9
6
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4900710
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900709 FACS IP Hamster IgG Log in to see 145-2C11 8
1 ABIN135085 FACS IP IHC WB Hamster IgG Log in to see 145-2C11 3
1 ABIN135091 FACS IP IHC WB Hamster IgG Log in to see 145-2C11 3
1 ABIN967370 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
1 ABIN1302512 Func ICC IHC (fro) FACS IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
1 ABIN967568 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
1 ABIN2658968 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
1 ABIN2659506 FACS IP WB PE-Cy7 Hamster IgG Log in to see 145-2C11 9
1 ABIN135095 FACS IHC (p) IP Biotin Rat IgG2b Log in to see C363-29B 3
1 ABIN2659906 ELISA (Capture) FACS IP WB PerCP Hamster IgG Log in to see 145-2C11 9
1 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
1 ABIN439836 FACS IHC IHC (fro) IHC (p) IP in vitro Biotin Rat IgG2b kappa Log in to see C363-29B (YCD3-1) 5
1 ABIN135092 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
1 ABIN135098 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
1 ABIN439833 FACS IHC (fro) IP in vitro Cy5 Syrian Hamster IgG kappa Log in to see 500-A2
1 ABIN439837 FACS IHC (p) in vitro IP IHC PE Rat IgG2b kappa Log in to see C363-29B (YCD3-1)
1 ABIN439838 FACS IHC (p) in vitro IP IHC Allophycocyanin Rat IgG2b kappa Log in to see C363-29B (YCD3-1)
1 ABIN439839 FACS IHC (p) in vitro IP IHC Cy5 Rat IgG2b kappa Log in to see C363-29B (YCD3-1)
1 ABIN439822 FACS IHC (fro) in vitro IP IHC WB FITC Armenian Hamster IgG kappa Log in to see 145-2C11
1 ABIN439835 FACS IHC IHC (fro) IHC (p) IP in vitro FITC Rat IgG2b kappa Log in to see C363-29B (YCD3-1)

Top referenced anti-CD3E antibody for Immunoprecipitation

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 423 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 10 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 96 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 37 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(494), (485), (120), (79), (49), (30), (28), (28), (28), (28), (21), (13), (9), (9), (8), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Hamster
(446), (222), (212), (80), (20), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(101), (85), (72), (39), (23), (22), (21), (21), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (12), (7), (7), (7), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(745), (323), (219), (198), (147), (137), (114), (94), (90), (42), (25), (18), (13), (10), (9), (9), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN4900710) Images
Specificity Detects mouse CD3 epsilon . Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon-chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen H-2Kb-specific mouse cytotoxic T-lymphocyte
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN4900710) Images back to top
Antigen
Alternative Name CD3 epsilon (CD3E Antibody Abstract)
Gene ID 916
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Hematopoietic Stem Cells, Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN4900710) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells, Immunoprecipitation, Cell depletion, T Cell Stimulation 0.1-0.6 μg/mL, CyTOF-reported
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN4900710) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD3E Antibody (ABIN4900710)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Gupta, Marcel, Talwar, Garg, R, Perumalsamy, Sarin, Shivashankar: "Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration." in: PLoS ONE, Vol. 7, Issue 9, pp. e43718, 2012 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Jones, Stumhofer, Foster, Twohig, Hertzog, Topley, Williams, Hunter, Jenkins, Wang, Jones: "Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 409-19, 2011 Method employed by authors: Biochemical Assay (BCA) (Sample species: Mouse (Murine)).

Jones, McLoughlin, Hammond, Parker, Williams, Malhotra, Scheller, Williams, Rose-John, Topley, Jones: "Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 4, pp. 2130-9, 2010 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Sinclair, Finlay, Feijoo, Cornish, Gray, Ager, Okkenhaug, Hagenbeek, Spits, Cantrell: "Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking." in: Nature immunology, Vol. 9, Issue 5, pp. 513-21, 2008 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Li, Kashiwamura, Ueda, Sekiyama, Okamura: "Protection of CD8+ T cells from activation-induced cell death by IL-18." in: Journal of leukocyte biology, Vol. 82, Issue 1, pp. 142-51, 2007 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Bheeshmachar, Purushotaman, Sade, Gunasekharan, Rangarajan, Sarin: "Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5041-50, 2006 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Ogilvie, Abelson, Hau, Vlasova, Blackshear, Bohjanen: "Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 2, pp. 953-61, 2005 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Kipnis, Cardon, Avidan, Lewitus, Mordechay, Rolls, Shani, Schwartz: "Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 27, pp. 6133-43, 2004 Method employed by authors: Functional Studies (Func) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN4900710) back to top
Supplier Images
Functional Studies (Func) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN4900710) Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster An...