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anti-Mouse (Murine) CD3E antibody for Immunoprecipitation

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Mouse (Murine)
392
244
61
46
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Armenian Hamster
204
161
156
77
32
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
459
175
174
133
68
64
52
45
34
24
20
10
8
6
4
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN967370
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008970579 ABIN967568 BR CyTox IHC (fro) FACS IF IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 13
0.0008970579 ABIN135085 FACS IP IHC WB Hamster IgG Log in to see 145-2C11 3
0.0008970579 ABIN135091 FACS IP IHC WB Hamster IgG Log in to see 145-2C11 3
0.0008970579 ABIN1302512 Func ICC IHC (fro) FACS IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.0008970579 ABIN2658968 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9
0.0008970579 ABIN2659506 FACS IP WB PE-Cy7 Hamster IgG Log in to see 145-2C11 9
0.0008970579 ABIN2659906 ELISA (Capture) FACS IP WB PerCP Hamster IgG Log in to see 145-2C11 9
0.0008970579 ABIN135095 FACS IHC (p) IP Biotin Rat IgG2b Log in to see C363-29B 3
0.0008970579 ABIN457410 Func ICC FACS IHC (fro) IP Hamster IgG epsilon subunit Log in to see 145-2C11 9
0.0008970579 ABIN457415 ICC FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
0.0008970579 ABIN288832 FACS IHC (p) IP Biotin Rat IgG2b Log in to see C365-29B
0.0008970579 ABIN135098 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
0.0008970579 ABIN135092 FACS IHC (p) IP Rat IgG2b Log in to see C363-29B 3
0.0008970579 ABIN288827 FACS IP Biotin Hamster IgG Log in to see 500-A2
0.0008970579 ABIN284221 FACS IHC (p) IP Rat IgG2b Log in to see C365-29B
0.0008970579 ABIN284217 FACS IP IHC WB Hamster IgG Log in to see 145-2C11
0.0008970579 ABIN1176869 BR CyTox FM IHC (fro) FACS IP WB Armenian Hamster IgG1, kappa Log in to see 145-2C11 10
0.0008970579 ABIN2689215 BR IHC (f) IHC (fro) FACS IHC (zinc) IP WB Armenian Hamster IgG1, kappa Log in to see 145 5
0.0008970579 ABIN2659507 FACS IP WB PE-Cy7 Hamster IgG Log in to see 145-2C11 9
0.0008970579 ABIN2658969 ELISA (Capture) ELISPOT (Capture) ICC CyTOF IP Neut IHC PE-Cy5 Hamster IgG Log in to see 145-2C11 9

Top referenced anti-CD3E antibody for Immunoprecipitation

Similar anti-CD3E Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 14 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 338 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 128 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(392), (244), (61), (46), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Armenian Hamster
(204), (161), (156), (77), (32), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(459), (175), (174), (133), (68), (64), (52), (45), (34), (24), (20), (10), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN967370) Images
Brand BD Pharmingen™
Characteristics 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Clone 145-2C11
Isotype IgG1, kappa

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN967370) Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CD3epsilon chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN967370) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN967370) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.

References for anti-CD3E Antibody (ABIN967370)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Martin, Lenardo: "Morphological, biochemical, and flow cytometric assays of apoptosis." in: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 14, pp. Unit 14.13, 2008

Kruisbeek, Shevach, Thornton: "Proliferative assays for T cell function." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.12, 2008

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994

Background publications

Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." in: International immunology, Vol. 9, Issue 7, pp. 935-44, 1997

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996

Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, 1996

Isakov, Wange, Burgess, Watts, Aebersold, Samelson: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN967370) back to top
Supplier Images
 image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967370) CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained ...
 image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967370) CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained ...
 image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN967370) CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained ...