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anti-Non-Human Primate CD3E antibody for Immunohistochemistry (Frozen Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human, Non-Human Primate
393
243
61
46
7
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Mouse
204
160
156
77
33
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
459
175
175
133
68
64
52
46
34
23
20
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN638434
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN489928 Func FACS IHC (fro) IP APC Mouse IgG1 Log in to see UCHT1 11
0.00088051456 ABIN489929 Func FACS IHC (fro) IP PerCP Mouse IgG1 Log in to see UCHT1 9
0.00088051456 ABIN775704 Func IHC (fro) FACS IP Mouse IgG1 Log in to see UCHT1 9

Top referenced anti-CD3E antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD3E Antibodies

Application / Reactivity Non-Human Primate Human
Flow Cytometry (FACS) 6 Antibodies 84 Antibodies
Functional Studies (Func) 4 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 25 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 66 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human, Non-Human Primate
(393), (243), (61), (46), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(204), (160), (156), (77), (33), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(459), (175), (175), (133), (68), (64), (52), (46), (34), (23), (20), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638434) Images
Specificity The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex. HLDA I, WS Code T 3HLDA III, WS Code T 126HLDA III, WS Code T 471HLDA VI, WS Code T 6T-CD3.1
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen human thymocytes followed by Sezary T cells
Clone UCHT1
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN638434) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-82 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN638434) Images back to top
Application Notes Flow Cytometry:
Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Immunohistochemistry (frozen sections)
Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Functional Application: The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN638434) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD3E Antibody (ABIN638434)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Siegers, Swamy, Fernández-Malavé, Minguet, Rathmann, Guardo, Pérez-Flores, Regueiro, Alarcón, Fisch, Schamel: "Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2537-44, 2007

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998

Fisch, Malkovsky, Braakman, Sturm, Bolhuis, Prieve, Sosman, Lam, Sondel: "Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis." in: The Journal of experimental medicine, Vol. 171, Issue 5, pp. 1567-79, 1990

Garson, Beverley, Coakham, Harper: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." in: Nature, Vol. 298, Issue 5872, pp. 375-7, 1982

Background publications

Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006

Lin, Liu, Chen, Wang, Medeiros, Hsu: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN638434) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN638434) Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific ...