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anti-Rat (Rattus) CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Epitope
AA 23-207
31
23
19
15
14
13
13
12
8
7
6
6
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
523
412
124
89
65
45
44
43
43
43
30
26
25
24
22
22
21
7
6
2
2
2
2
1
1
Host
Rabbit
360
224
211
79
35
21
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
97
82
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
692
297
218
168
128
91
88
84
84
34
25
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3043805
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13397987 ABIN1385705 IF (p) IHC (p) WB Rabbit IgG AA 21-71 Log in to see Polyclonal
0.13397987 ABIN1871626 IHC WB Rabbit IgG Log in to see Polyclonal
0.13397987 ABIN3022492 IHC WB Rabbit IgG Log in to see Polyclonal
0.13397987 ABIN2425325 WB Rabbit IgG Log in to see Polyclonal
0.13397987 ABIN2404594 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011731815 ABIN672921 IF (p) IHC (p) WB Rabbit IgG AA 132-170 Log in to see Polyclonal
0.0011731815 ABIN1414551 IHC (p) WB HRP Rabbit IgG AA 21-71 Log in to see Polyclonal
0.0011731815 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0011731815 ABIN1396674 IHC (p) WB Biotin Rabbit IgG AA 21-71 Log in to see Polyclonal
0.0011731815 ABIN871723 WB Rabbit AA 197-201 Log in to see Polyclonal
0.0011731815 ABIN672923 IHC (p) WB Biotin Rabbit IgG AA 132-170 Log in to see Polyclonal
0.0011731815 ABIN672930 IHC (p) WB HRP Rabbit IgG AA 132-170 Log in to see Polyclonal
0.0011731815 ABIN2962185 IHC (fro) IHC (p) ELISA WB Rabbit AA 156-168 Log in to see Polyclonal
0.0011731815 ABIN2561723 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0011731815 ABIN3026772 FACS IP WB Rabbit IgG Log in to see Polyclonal
0.0011731815 ABIN2749613 ELISA WB FITC Rabbit IgG Middle Region Log in to see Polyclonal
0.0011731815 ABIN2749612 IP IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
0.0011731815 ABIN2749614 ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0011731815 ABIN2749615 ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0011731815 ABIN4291565 IHC WB Mouse IgG1 Log in to see PC3-188A

Similar anti-CD3E Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 14 Antibodies
Flow Cytometry (FACS) 27 Antibodies 469 Antibodies
Immunocytochemistry (ICC) 25 Antibodies 52 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 68 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 23-207
(31), (23), (19), (15), (14), (13), (13), (12), (8), (7), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(523), (412), (124), (89), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (6), (2), (2), (2), (2), (1), (1)
Host Rabbit
(360), (224), (211), (79), (35), (21), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(97), (82), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(692), (297), (218), (168), (128), (91), (88), (84), (84), (34), (25), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain
Purification Immunogen affinity purified.
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Gene ID 916
UniProt P07766
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-6.JPG IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-7.JPG IHC(F): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml WB : Re...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml Lane 1:...
Immunohistochemistry (IHC) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (AA 23-207) (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Rat Spleen Tissue