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anti-Cat (Feline) CD4 antibody for Western Blotting

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Cat (Feline)
1162
610
233
68
66
39
34
22
22
21
19
18
16
15
15
12
10
10
9
9
6
5
5
5
4
2
2
2
1
1
1
1
Host
Mouse
1313
568
271
10
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
258
221
163
100
47
47
45
40
36
34
33
29
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1662
462
427
328
305
283
150
143
84
61
39
38
29
29
22
19
18
14
14
11
9
7
6
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN957928
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN956669 ICC FACS ELISA WB Goat IgG Extracellular Domain Log in to see Polyclonal
1 ABIN4900716 FACS ICC WB Goat IgG Log in to see Polyclonal

Similar anti-CD4 Antibodies

Application / Reactivity Cat (Feline)
Blocking Peptide (BP) 2 Antibodies
ELISA 2 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Cat (Feline)
(1162), (610), (233), (68), (66), (39), (34), (22), (22), (21), (19), (18), (16), (15), (15), (12), (10), (10), (9), (9), (6), (5), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1313), (568), (271), (10), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(258), (221), (163), (100), (47), (47), (45), (40), (36), (34), (33), (29), (27), (23), (21), (21), (19), (19), (18), (18), (18), (18), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1662), (462), (427), (328), (305), (283), (150), (143), (84), (61), (39), (38), (29), (29), (22), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling Images
Specificity Feline CD4.
Purification Protein G purified
Sterility 0.2 μm filtered
Immunogen Purified, NS0-derived, recombinant feline CD4 (rfeCD4, aa 25 - 413, Accession # BAA19124).

Type of Immunogen: Recombinant protein
Clone 247109
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule
Gene ID 920
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Application Notes Approved: ELISA (0.5 - 1 μg/mL), ICC (25 μg/mL), IHC, WB (1 - 2 μg/mL)

Usage: Western blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect feline CD4. Using a colorimetric detection system, the detection limit for rfeCD4 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Immunocytochemistry-This antibody was used at a concentration of 25 μg/mL with appropriate secondary reagents to detect CD4 in paraformaldehyde-fixed feline PBMC. is recommended. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect feline CD4. The detection limit for rfeCD4 is approximately 3 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Format Lyophilized
Reconstitution Sterile PBS. Resuspend to 200 μL
Concentration Lot specific
Buffer Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose
Storage -20 °C
Storage Comment Store at -20°C.