anti-Ferret CD4 antibody for ELISA

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
AA 1-401
66
64
21
19
15
15
15
13
12
10
8
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Ferret
1220
632
234
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
8
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1376
587
281
11
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA
1744
490
445
353
322
309
157
155
88
78
39
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2001471
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2001473 ELISA Rabbit IgG AA 1-401 Log in to see Polyclonal 1
1 ABIN5506264 ELISA Rabbit IgG AA 1-401 Log in to see 108

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope AA 1-401
(66), (64), (21), (19), (15), (15), (15), (13), (12), (10), (8), (8), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Ferret
(1220), (632), (234), (68), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (8), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1376), (587), (281), (11), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA
(1744), (490), (445), (353), (322), (309), (157), (155), (88), (78), (39), (38), (31), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN2001471) Images
Specificity Ferret CD4
No cross-reactivity in ELISA with
Mouse CD4
Human CD4
Human cell lysate (293 cell line)
No Cross-Reactivity Human, Mouse (Murine)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD4 (rF CD4, ABIN2008790 ABS50090.1, Met 1-Leu 401,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Ferret CD4 protein (ABIN2008790)
Clone 4G5E3
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN2001471) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree, however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.
UniProt ABS50090.1
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN2001471) Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret CD4. The detection limit for Ferret CD4 is approximately 0.078 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN2001471) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-CD4 antibody (ABIN2001471)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Biddison, Shaw: "CD4 expression and function in HLA class II-specific T cells." in: Immunological reviews, Vol. 109, pp. 5-15, 1989