No cross-reactivity in ELISA with Human CD4 Mouse CD4
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD4 (rF CD4, ABS50090.1, Met 1-Leu 401) and conjugated with PE under optimum conditions, the unreacted PE was removed.
T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single- pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoproteinexpressed on the surface of T helper cells, regulatory T cells,monocytes, macrophages, anddendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 before being named CD4 in 1984. CD4 is aco-receptorthat assists theT cell receptor (TCR) to activate its T cell following an interaction with anantigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly withMHC class II molecules on the surface of the antigen presenting cell via itsextracellulardomain. CD4 is a primary receptor used byHIV-1to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.