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anti-Human CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Human
1182
652
239
72
56
43
26
23
22
21
19
15
15
15
11
10
9
8
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1352
624
269
9
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to FITC
275
238
172
106
46
45
43
36
36
35
34
33
25
22
21
21
21
19
18
18
18
18
16
15
13
13
12
8
8
7
7
7
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1740
462
427
324
310
294
181
150
118
68
33
28
28
27
22
19
18
14
14
11
9
7
6
6
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN2213334
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.739399 ABIN118488 FACS PE Mouse IgG1 Log in to see RPA-T4 2
13.739399 ABIN120115 FACS IHC (fro) Rat IgG1 Log in to see YNB46-1-8 2
13.739399 ABIN118485 FACS FITC Mouse IgG1 Log in to see RPA-T4 2
13.739399 ABIN1688441 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP35 32
13.739399 ABIN118487 FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 2
13.739399 ABIN1106337 FACS Mouse IgG1 Log in to see 4H5 2
13.739399 ABIN4897871 FACS Allophycocyanin Mouse IgG2a Extracellular Domain Log in to see 11830 3
13.739399 ABIN4897872 FACS Allophycocyanin Mouse IgG2a Extracellular Domain Log in to see 11830 3
13.739399 ABIN4897874 FACS PerCP Mouse IgG2a Extracellular Domain Log in to see 11830 1
13.739399 ABIN4897875 FACS Fluorescein Mouse IgG2a Extracellular Domain Log in to see 11830 2
13.739399 ABIN4897873 FACS PerCP Mouse IgG2a Extracellular Domain Log in to see 11830 1
13.739399 ABIN4897876 FACS Fluorescein Mouse IgG2a Extracellular Domain Log in to see 11830 2
13.739399 ABIN4897881 FACS PE Mouse IgG2a Extracellular Domain Log in to see 11830 3
13.739399 ABIN4897882 FACS PE Mouse IgG2a Extracellular Domain Log in to see 11830 3
13.739399 ABIN1724630 FACS ELISA Mouse IgG1 Log in to see B486A1 1
13.739399 ABIN259655 FACS WB PerCP Mouse IgG1 Log in to see MEM-241 6
13.739399 ABIN4292796 BP FACS IHC IHC (fro) in vitro Neut Mouse IgG1 kappa Log in to see RPA-T4 11
13.739399 ABIN1106351 FACS PE Mouse IgG2a Log in to see B-A1
13.739399 ABIN4260770 FACS Allophycocyanin Mouse IgG1 kappa Log in to see RPA-T4 1
13.739399 ABIN192276 FACS WB PE-DyLight 594 Mouse IgG1 N-Term Log in to see MEM-241 9

Top referenced anti-CD4 antibody for Flow Cytometry

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General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human
(1182), (652), (239), (72), (56), (43), (26), (23), (22), (21), (19), (15), (15), (15), (11), (10), (9), (8), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1352), (624), (269), (9), (4), (1)
Clonality
Monoclonal
Conjugate This CD4 antibody is conjugated to FITC
(275), (238), (172), (106), (46), (45), (43), (36), (36), (35), (34), (33), (25), (22), (21), (21), (21), (19), (18), (18), (18), (18), (16), (15), (13), (13), (12), (8), (8), (7), (7), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1740), (462), (427), (324), (310), (294), (181), (150), (118), (68), (33), (28), (28), (27), (22), (19), (18), (14), (14), (11), (9), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2 °C-8 °C.
Expiry Date 12 months

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule)  (FITC) (ABIN2213334) anti-CD4 Molecule (CD4) antibody (FITC)
'Independent Validation' Badge
Lot Number HG08FE1721
Method validated Flow Cytometry
Positive Control peripheral blood mononuclear cells
Negative Control peripheral blood mononuclear cells
Notes This antibody performed well and is comparable to anti-CD4-FITC from BD Pharmingen (see data below)
Primary Antibody
  • Antigen: human CD4 (CD4)
  • Catalog number: ABIN2104933
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  • Supplier catalog number: Log in to see
  • Lot number: HG08FE1721
  • Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA
Controls
  • Positive control: peripheral blood mononuclear cells stained with anti-CD4
  • Negative control: peripheral blood mononuclear cells that are CD4 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protocol
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 5µl of anti-CD4-FITC (Sino Biological) or 20µl of anti-CD4-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validation Images
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC (red),...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC from B...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...