anti-Human CD4 antibody for Functional Studies

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
Domain D1
66
64
21
18
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
1218
632
234
69
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1378
587
280
10
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
1743
490
442
354
321
309
157
155
87
77
39
38
30
30
25
19
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN125679
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120586 Func FACS IP Mouse IgG2a Log in to see MEM-115 1
1 ABIN94096 Func FACS IP Mouse IgG2a Domain D1 Log in to see MEM-115 6
1 ABIN118486 Func FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 12
1 ABIN1106338 Func FACS IHC (p) Mouse IgG2a Log in to see B-A1
1 ABIN125999 Func FACS IP Mouse IgG2a Log in to see MEM-115 3
1 ABIN2662087 Func ICC FACS IF ICFC IP WB Pacific Blue Mouse IgG2b kappa Log in to see OKT4 8
1 ABIN2662088 Func ICC FACS IF ICFC IP WB Pacific Blue Mouse IgG2b kappa Log in to see OKT4 8
1 ABIN609870 Func FACS ELISA Biotin Mouse IgG1 Log in to see QS4120
1 ABIN206939 Inhibition BR FACS Func IP Neut Mouse IgG2a Log in to see MEM-115
1 ABIN609869 Func FACS ELISA Mouse IgG1 Log in to see QS4120
1 ABIN203855 Func FACS IHC Biotin Mouse IgG1 Log in to see
1 ABIN206889 Inhibition BR FACS Func IP Neut Mouse IgG2a Log in to see MEM-115
1 ABIN2480204 Func IHC (fro) FACS Mouse IgG1 Log in to see RPA-T4 1
1 ABIN1177369 Func FACS Mouse IgG1, kappa Log in to see RPA-T4
1 ABIN238364 EIA Func FACS IHC (fro) WB Mouse IgG1 Log in to see 13B8-2
1 ABIN600642 Func IHC (fro) FACS IHC (p) Mouse IgG1 Log in to see
1 ABIN4292844 CyTOF ELISA FACS Func ICC IF Mouse IgG1 Log in to see QS4120
1 ABIN1722590 Func FACS IHC Mouse IgG2a Log in to see B-A1
1 ABIN3199820 FACS Func IP Mouse IgG2a Log in to see MEM-115
1 ABIN2567616 Func ICC FACS IF IP WB Mouse IgG2b kappa Log in to see OKT4

Top referenced anti-CD4 antibody for Functional Studies

Similar anti-CD4 Antibodies

Application / Reactivity Human
Activation (Act) 3 Antibodies
Biochemical Assay (BCA) 6 Antibodies
Blocking Antibody (Inhibition) 4 Antibodies
Blocking Peptide (BP) 21 Antibodies
Blocking Reagent (BR) 12 Antibodies
Complement Mediated Cell Depletion (CMCD) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 17 Antibodies
Depletion (Dep) 4 Antibodies
ELISA 260 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 821 Antibodies
Functional Studies (Func) 30 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 39 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain D1
(66), (64), (21), (18), (15), (15), (15), (13), (12), (10), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1218), (632), (234), (69), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1378), (587), (280), (10), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(1743), (490), (442), (354), (321), (309), (157), (155), (87), (77), (39), (38), (30), (30), (25), (19), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN125679) Images
Specificity The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
HLDA V, WS Code T T-CD04.09
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-115
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN125679) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN125679) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN125679) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

ProductDetails: References for anti-CD4 antibody (ABIN125679)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Bosze, Caccamo, Majer, Mezo, Dieli, Hudecz: "In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis." in: Biopolymers, Vol. 76, Issue 6, pp. 467-76, 2004

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003

Singer, Scott, Kawka, Chin, Daugherty, DeMartino, DiSalvo, Gould, Lineberger, Malkowitz, Miller, Mitnaul, Siciliano, Staruch, Williams, Zweerink, Springer: "CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells." in: Journal of virology, Vol. 75, Issue 8, pp. 3779-90, 2001

Background publications

Clapham, McKnight: "Cell surface receptors, virus entry and tropism of primate lentiviruses." in: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, 2002

Foti, Phelouzat, Holm, Rasmusson, Carpentier: "p56Lck anchors CD4 to distinct microdomains on microvilli." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, 2002

Millán, Cerny, Horejsi, Alonso: "CD4 segregates into specific detergent-resistant T-cell membrane microdomains." in: Tissue antigens, Vol. 53, Issue 1, pp. 33-40, 1999

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN125679) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-CD4 antibody (CD4 Molecule) (Domain D1) (ABIN125679) Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy co...