anti-Human CD4 antibody for Immunofluorescence

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Human, Mouse (Murine)
1171
618
234
68
67
40
36
22
22
19
18
18
16
15
15
12
12
10
10
9
6
5
5
5
4
3
2
2
1
1
1
1
Host
Rabbit
1324
573
272
10
4
1
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
258
222
166
100
48
48
46
40
36
34
33
30
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1676
467
430
333
308
289
152
141
87
61
39
38
30
30
23
19
18
14
14
11
9
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
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Catalog No. ABIN1532559
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.16076 ABIN439030 ICC IF IHC IHC (p) SimWes WB Rabbit C-Term, AA 400-458 Log in to see Polyclonal 11
12.16076 ABIN1531544 IF IHC ELISA Rabbit IgG pSer433 Log in to see Polyclonal
12.16076 ABIN2452385 IF WB Mouse IgG2b Log in to see 3B4
12.16076 ABIN2452331 IF WB Mouse IgG2a Log in to see 4A11
12.16076 ABIN2452332 IF WB Mouse IgG1 Log in to see 9G7
1 ABIN1106339 IHC (fro) IF Mouse IgG1 Log in to see CA-4 2
1 ABIN2144254 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144257 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144256 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144346 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144255 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144344 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144349 ICC FACS IF PE-Cy5 Mouse IgG2a Log in to see 4AEDU-2 2
1 ABIN2144347 ICC FACS IF PE-Cy5 Mouse IgG2a Log in to see 4AEDU-2 2
1 ABIN2144461 FACS IF IP IHC FITC Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144757 ICC FACS IF PE Mouse IgG2a Log in to see 4AEDU-2 2
1 ABIN2144750 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144752 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
1 ABIN2144754 ICC FACS IF PE Mouse IgG2a Log in to see 4AEDU-2 2
1 ABIN2144463 ICC FACS IF FITC Mouse IgG2a Log in to see 4AEDU-2 2

Top referenced anti-CD4 antibody for Immunofluorescence

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 47 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 483 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 128 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human, Mouse (Murine)
(1171), (618), (234), (68), (67), (40), (36), (22), (22), (19), (18), (18), (16), (15), (15), (12), (12), (10), (10), (9), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1324), (573), (272), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(258), (222), (166), (100), (48), (48), (46), (40), (36), (34), (33), (30), (27), (23), (21), (21), (19), (19), (18), (18), (18), (18), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1676), (467), (430), (333), (308), (289), (152), (141), (87), (61), (39), (38), (30), (30), (23), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images
Specificity CD4 Antibody detects endogenous levels of total CD4 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD4.
Isotype IgG

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Molecular weight of target protein: 51 kDa
Synonyms: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 precursor
Molecular Weight 51 kDa
Gene ID 920
OMIM 186940
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.631659 (NCBI Gene Symbol: CD4)

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CD4 antibody (ABIN1532559)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Yamada, Yamaguchi, Asano, Fujita, Kobayashi, Kasai: "Th17-cells in atopic dermatitis stimulate orthodontic root resorption." in: Oral diseases, 2013 Method employed by authors: Immunofluorescence (IF) (1:100). Further details: For double immunofluorescence staining, the sections were deparaffinized and washed in TBS, and then were incubated with a polyclonal anti-rabbit CD4 antibody (Working dilution, 1:100) for 18 hours at 4°C.

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) back to top
Supplier Images
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Western blot analysis of extracts from COLO205 cells, using CD4 (Ab-433) Antibody. Th...
Immunohistochemistry (IHC) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD4 (Ab-...
Immunofluorescence (IF) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Immunofluorescence analysis of HeLa cells, using CD4 (Ab-433) Antibody. The picture o...