anti-Human CD4 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Human
1219
633
235
68
67
44
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1378
587
284
11
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
265
227
168
103
50
49
47
41
36
34
34
30
28
26
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
1750
493
447
356
324
309
160
155
88
78
39
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN965796
$ 295.63
Plus shipping costs $45.00

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human
(1219), (633), (235), (68), (67), (44), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1378), (587), (284), (11), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(265), (227), (168), (103), (50), (49), (47), (41), (36), (34), (34), (30), (28), (26), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
(1750), (493), (447), (356), (324), (309), (160), (155), (88), (78), (39), (38), (31), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN965796) Images
Specificity This antibody reacts with human CD4 antigen, a single transmembrane 59 kD protein. This antibody serves as a receptor for HIV virus.
Purification Purified.
Immunogen CEM cells.
Clone B379 (JOU 4B7F4)
Isotype IgM

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN965796) Images back to top
Antigen
Gene ID 920
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN965796) Images back to top
Application Notes Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.
Comment

Recommended Positive Control: Human Lymph Nodes and Tonsils.

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN965796) Images back to top
Format Liquid
Concentration 0.4 mg/ml.
Buffer 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD4 antibody (ABIN965796)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Gaubin, Autiero, Houlgatte, Basmaciogullari, Auffray, Piatier-Tonneau: "Molecular basis of T lymphocyte CD4 antigen functions." in: European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, Vol. 34, Issue 9, pp. 723-8, 1997

Mazerolles, Auffray, Fischer: "Down regulation of T-cell adhesion by CD4." in: Human immunology, Vol. 31, Issue 1, pp. 40-6, 1991

Veillette, Bookman, Horak, Samelson, Bolen: "Signal transduction through the CD4 receptor involves the activation of the internal membrane tyrosine-protein kinase p56lck." in: Nature, Vol. 338, Issue 6212, pp. 257-9, 1989