anti-Human CD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Human
1250
656
235
78
68
41
36
28
27
26
22
21
19
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1422
610
283
16
6
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
264
232
170
108
54
54
54
48
36
34
34
33
30
26
22
21
21
20
19
19
18
18
18
18
16
15
15
13
13
9
9
8
8
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1822
495
453
352
323
311
161
156
98
78
41
39
38
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120115
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN781715 IHC (fro) FACS Mouse IgG2a Log in to see EDU-2 1
1 ABIN118487 FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 2
1 ABIN1106336 IHC (fro) FACS IHC (p) Mouse IgG2a Log in to see Edu-2 2
1 ABIN1106339 IHC (fro) IF Mouse IgG1 Log in to see CA-4 2
1 ABIN4292796 BP FACS IHC IHC (fro) in vitro Neut Mouse IgG1 kappa Log in to see RPA-T4 12
1 ABIN1450194 IHC (fro) FACS Rat IgG1 Log in to see YNB46-1-8 1
1 ABIN181799 FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 3
1 ABIN118486 Func FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 12
1 ABIN2480254 IHC (fro) FACS Rat IgG1 Log in to see YNB46-1-8 2
1 ABIN965795 IHC (fro) FACS Mouse IgG2a Log in to see B486 (EDU-2) 5
1 ABIN3043630 IHC (fro) Mouse IgG1 Log in to see CA-4 5
1 ABIN255883 FACS IHC (fro) IHC Rat IgG1 Log in to see YNB46-1-8
1 ABIN371523 FACS IHC (fro) IP Mouse IgG1 Log in to see RFT-4g 1
1 ABIN965796 IHC (af) IHC (fro) FACS IF Mouse IgM Log in to see B379 (JOU 4B7F4) 3
1 ABIN2480187 IHC (fro) FACS Mouse IgG1 Log in to see RPA-T4 1
1 ABIN440354 FACS ICC IHC IHC (fro) IP WB FITC Mouse IgG1 Log in to see RFT-4g
1 ABIN440355 FACS ICC IHC IHC (fro) IP WB Biotin Mouse IgG1 Log in to see RFT-4g
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6
1 ABIN4292815 BP FACS IHC IHC (fro) Neut Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T4
1 ABIN4292810 BP FACS IHC IHC (fro) Neut DyLight 405 Mouse IgG1 kappa Log in to see RPA-T4

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human
(1250), (656), (235), (78), (68), (41), (36), (28), (27), (26), (22), (21), (19), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1422), (610), (283), (16), (6), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(264), (232), (170), (108), (54), (54), (54), (48), (36), (34), (34), (33), (30), (26), (22), (21), (21), (20), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (9), (9), (8), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1822), (495), (453), (352), (323), (311), (161), (156), (98), (78), (41), (39), (38), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120115) Images
Specificity This antibody recognises the Human CD4 cell surface antigen, a 55 kDa molecule expressed by helper T lymphocytes and by monocytes. This antibody blocks syncytia formation of U937 cells infected with HIV.
Purification Protein G Affinity Chromatography
Immunogen Rat cells transfected with Human CD4. Spleen cells from immunised PVG-RT1u rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Clone YNB46-1-8
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN120115) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN120115) Images back to top
Application Notes Flow Cytometry (Use 10 μL of 1/100 - 1/200 diluted antibody to label 10^6 cells in 100 μL). Immunohistochemistry on frozen sections (1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN120115) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 antibody (ABIN120115)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Hirbod, Bailey, Agot, Moses, Ndinya-Achola, Murugu, Andersson, Nilsson, Broliden: "Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men." in: The American journal of pathology, Vol. 176, Issue 6, pp. 2798-805, 2010

Yawalkar, Hunger, Buri, Schmid, Egli, Brand, Mueller, Pichler, Braathen et al.: "A comparative study of the expression of cytotoxic proteins in allergic contact dermatitis and psoriasis: spongiotic skin lesions in allergic contact dermatitis are highly infiltrated by T cells ..." in: The American journal of pathology, Vol. 158, Issue 3, pp. 803-8, 2001

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120115) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (ABIN120115) Staining of human peripheral blood lymphocytes with Rat anti Human CD4 antibody.