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|Application / Reactivity||Human|
|Activation (Act)||3 Antibodies|
|Biochemical Assay (BCA)||6 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Reagent (BR)||9 Antibodies|
|Complement Mediated Cell Depletion (CMCD)||2 Antibodies|
|Depletion (Dep)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||570 Antibodies|
|Functional Studies (Func)||31 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||38 Antibodies|
|Immunofluorescence (IF)||92 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN120115) Images
|Specificity||This antibody recognises the Human CD4 cell surface antigen, a 55 kDa molecule expressed by helper T lymphocytes and by monocytes. This antibody blocks syncytia formation of U937 cells infected with HIV.|
|Purification||Protein G Affinity Chromatography|
|Immunogen||Rat cells transfected with Human CD4. Spleen cells from immunised PVG-RT1u rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN120115) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN120115) Images back to top
Flow Cytometry (Use 10 μL of 1/100 - 1/200 diluted antibody to label 10^6 cells in 100 μL). Immunohistochemistry on frozen sections (1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN120115) Images back to top
|Buffer||PBS, 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD4 Antibody (ABIN120115)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Hirbod, Bailey, Agot et al.: "Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men." in: The American journal of pathology, Vol. 176, Issue 6, pp. 2798-805, 2010 (PubMed).
Yawalkar, Hunger, Buri et al.: "A comparative study of the expression of cytotoxic proteins in allergic contact dermatitis and psoriasis: spongiotic skin lesions in allergic contact dermatitis are highly infiltrated by T cells ..." in: The American journal of pathology, Vol. 158, Issue 3, pp. 803-8, 2001 (PubMed).
ImagesProduct Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN120115) back to top