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anti-Human CD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Human
940
592
211
60
44
25
23
22
21
15
13
9
8
7
7
6
5
5
3
3
2
2
2
2
1
1
Host
Rat
1113
549
189
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
255
218
150
114
37
36
24
24
21
20
19
16
15
15
14
13
9
8
8
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1383
348
330
257
208
173
159
91
72
69
33
28
27
18
14
11
10
9
7
6
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN120115
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008828464 ABIN118487 FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 2
0.0008828464 ABIN781715 IHC (fro) FACS Mouse IgG2a Log in to see EDU-2 1
0.0008828464 ABIN1106336 IHC (fro) FACS IHC (p) Mouse IgG2a Log in to see Edu-2 2
0.0008828464 ABIN1106339 IHC (fro) IF Mouse IgG1 Log in to see CA-4 2
0.0008828464 ABIN181799 FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 3
0.0008828464 ABIN1450194 IHC (fro) FACS Rat IgG1 Log in to see YNB46-1-8 1
0.0008828464 ABIN118486 Func FACS IHC (fro) Mouse IgG1 Log in to see RPA-T4 12
0.0008828464 ABIN371523 FACS IHC (fro) IP Mouse IgG1 Log in to see RFT-4g 1
0.0008828464 ABIN965795 IHC (fro) FACS Mouse IgG2a Log in to see B486 (EDU-2) 5
0.0008828464 ABIN284266 FACS IHC (fro) ELISA Mouse IgG1 Log in to see QS4120
0.0008828464 ABIN2480254 IHC (fro) FACS Rat IgG1 Log in to see YNB46-1-8 2
0.0008828464 ABIN1499795 IHC (fro) Mouse IgG1 Log in to see CA-4
0.0008828464 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6
0.0008828464 ABIN205540 FACS IHC (fro) Mouse IgG2b Log in to see OKT4
0.0008828464 ABIN211777 FACS IHC (fro) Rat IgG1 Log in to see YNB46-1-8
0.0008828464 ABIN3043630 IHC (fro) Mouse IgG1 Log in to see CA-4
0.0008828464 ABIN600645 IHC (fro) FACS IF Mouse IgM Log in to see
0.0008828464 ABIN345882 FACS IHC (fro) Mouse IgG1 Log in to see M504505
0.0008828464 ABIN211709 FACS IHC (fro) Mouse IgG1 Log in to see
0.0008828464 ABIN1100598 IHC (fro) FACS IHC (p) Mouse IgG2a Log in to see Edu-2

Top referenced anti-CD4 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Human
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Depletion (Dep) 4 Antibodies
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Functional Studies (Func) 31 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunofluorescence (IF) 92 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human
(940), (592), (211), (60), (44), (25), (23), (22), (21), (15), (13), (9), (8), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rat
(1113), (549), (189), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(255), (218), (150), (114), (37), (36), (24), (24), (21), (20), (19), (16), (15), (15), (14), (13), (9), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1383), (348), (330), (257), (208), (173), (159), (91), (72), (69), (33), (28), (27), (18), (14), (11), (10), (9), (7), (6), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN120115) Images
Specificity This antibody recognises the Human CD4 cell surface antigen, a 55 kDa molecule expressed by helper T lymphocytes and by monocytes. This antibody blocks syncytia formation of U937 cells infected with HIV.
Purification Protein G Affinity Chromatography
Immunogen Rat cells transfected with Human CD4. Spleen cells from immunised PVG-RT1u rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Clone YNB46-1-8
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN120115) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN120115) Images back to top
Application Notes Flow Cytometry (Use 10 μL of 1/100 - 1/200 diluted antibody to label 10^6 cells in 100 μL). Immunohistochemistry on frozen sections (1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN120115) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 Antibody (ABIN120115)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Hirbod, Bailey, Agot, Moses, Ndinya-Achola, Murugu, Andersson, Nilsson, Broliden: "Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men." in: The American journal of pathology, Vol. 176, Issue 6, pp. 2798-805, 2010

Yawalkar, Hunger, Buri, Schmid, Egli, Brand, Mueller, Pichler, Braathen et al.: "A comparative study of the expression of cytotoxic proteins in allergic contact dermatitis and psoriasis: spongiotic skin lesions in allergic contact dermatitis are highly infiltrated by T cells ..." in: The American journal of pathology, Vol. 158, Issue 3, pp. 803-8, 2001

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN120115) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN120115) Staining of human peripheral blood lymphocytes with Rat anti Human CD4 antibody.