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anti-Human CD4 antibody for Immunoprecipitation

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
Domain D1
77
40
22
22
13
13
13
10
8
8
7
7
7
7
6
6
6
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1197
694
240
69
52
45
25
23
22
21
19
15
14
12
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1361
667
276
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
284
239
176
114
44
42
42
39
39
38
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
1808
436
399
356
309
299
179
136
100
69
33
28
28
27
22
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN94096
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.5094592 ABIN125679 Func FACS IP Mouse IgG2a Domain D1 Log in to see MEM-115 6
0.00089556724 ABIN238451 FACS IP Mouse IgM Log in to see MEM-16 4
0.00089556724 ABIN120586 Func FACS IP Mouse IgG2a Log in to see MEM-115 1
0.00089556724 ABIN94103 FACS IP Mouse IgM Domain D1 Log in to see MEM-16 4
0.00089556724 ABIN2144256 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144346 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144255 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144254 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144257 FACS IF IP IHC Biotin Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144344 FACS IF IP IHC PE-Cy5 Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2145041 FACS IF IP IHC Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2145042 FACS IF IP IHC Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144459 FACS IF IP IHC FITC Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144750 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN125999 Func FACS IP Mouse IgG2a Log in to see MEM-115 3
0.00089556724 ABIN2144461 FACS IF IP IHC FITC Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2144752 FACS IF IP IHC PE Mouse IgG2b Log in to see 4AOKT4 2
0.00089556724 ABIN2657780 FACS IP Alexa Fluor 647 Mouse IgG2b kappa Log in to see OKT4 7
0.00089556724 ABIN2663446 FACS IP IHC WB PE Mouse IgG2b kappa Log in to see OKT4 8
0.00089556724 ABIN288968 FACS IP IHC Biotin Mouse IgG1 Log in to see RFT-4G

Top referenced anti-CD4 antibody for Immunoprecipitation

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Application / Reactivity Human
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Depletion (Dep) 4 Antibodies
ELISA 249 Antibodies
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General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain D1
(77), (40), (22), (22), (13), (13), (13), (10), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1197), (694), (240), (69), (52), (45), (25), (23), (22), (21), (19), (15), (14), (12), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1361), (667), (276), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(284), (239), (176), (114), (44), (42), (42), (39), (39), (38), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(1808), (436), (399), (356), (309), (299), (179), (136), (100), (69), (33), (28), (28), (27), (22), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN94096) Images
Specificity The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (,helper, T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.HLDA V, WS Code T T-CD04.09
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-115
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN94096) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 AA, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction.Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN94096) Images back to top
Application Notes Immunoprecipitation: excellent
Functional Application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions.
Flow Cytometry: Recommended dilution: 3 µg/mL
Application notes: Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN94096) Images back to top
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD4 Antibody (ABIN94096)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Bosze, Caccamo, Majer, Mezo, Dieli, Hudecz: "In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis." in: Biopolymers, Vol. 76, Issue 6, pp. 467-76, 2004

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003

Singer, Scott, Kawka, Chin, Daugherty, DeMartino, DiSalvo, Gould, Lineberger, Malkowitz, Miller, Mitnaul, Siciliano, Staruch, Williams, Zweerink, Springer: "CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells." in: Journal of virology, Vol. 75, Issue 8, pp. 3779-90, 2001

Background publications

Clapham, McKnight: "Cell surface receptors, virus entry and tropism of primate lentiviruses." in: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, 2002

Foti, Phelouzat, Holm, Rasmusson, Carpentier: "p56Lck anchors CD4 to distinct microdomains on microvilli." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, 2002

Millán, Cerny, Horejsi, Alonso: "CD4 segregates into specific detergent-resistant T-cell membrane microdomains." in: Tissue antigens, Vol. 53, Issue 1, pp. 33-40, 1999

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN94096) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-CD4 antibody (CD4 Molecule) (Domain D1) (ABIN94096) Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy co...