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anti-Mouse (Murine) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
Non-Polymorphic Site
77
22
19
13
13
13
10
8
8
7
7
6
6
6
5
5
5
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
941
591
211
60
44
25
23
22
21
15
13
9
8
7
7
6
5
5
3
3
2
2
2
2
1
1
Host
Rat
1113
549
189
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to FITC
254
218
150
114
37
36
24
24
21
20
19
16
15
15
14
13
9
8
8
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1383
348
330
257
208
174
159
91
72
69
33
28
27
18
14
11
10
9
7
6
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN118164
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN114145 FACS Biotin Rat IgG2a Log in to see CT-CD4 6
0.3068567 ABIN114148 FACS FITC Rat IgG2a Log in to see CT-CD4 7
0.3068567 ABIN114150 FACS PE Rat IgG2a Log in to see CT-CD4 7
0.3068567 ABIN114151 FACS PE Rat IgG2a Log in to see CT-CD4 7
0.3068567 ABIN114143 FACS APC Rat IgG2a Log in to see CT-CD4 10
0.3068567 ABIN118113 Func FACS IHC (fro) Rat IgG2b Log in to see YTS191-1 3
0.3068567 ABIN118114 FACS Rat IgG2b Log in to see YTS191-1 3
0.3068567 ABIN118115 FACS IHC (fro) Rat IgG2b Log in to see YTS191-1 15
0.3068567 ABIN254876 FACS Allophycocyanin Rat IgG2b Log in to see YTS191-1
0.3068567 ABIN2658865 FACS APC Rat IgG2b kappa Log in to see GK1-5 9
0.3068567 ABIN2658863 FACS APC Rat IgG2a kappa Log in to see RM4-5 8
0.3068567 ABIN2658972 FACS IP IHC WB PE-Cy5 Rat IgG2a kappa Log in to see RM4-5 8
0.3068567 ABIN2658974 FACS PE-Cy5 Rat IgG2a kappa Log in to see H129-19 6
0.3068567 ABIN2658977 FACS PE-Cy5 Rat IgG2b kappa Log in to see GK1-5 9
0.3068567 ABIN2659260 FACS PE-Cy7 Rat IgG2b kappa Log in to see RM4-4 4
0.3068567 ABIN2659512 FACS IF IHC PE-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
0.3068567 ABIN2659516 FACS PE-Cy7 Rat IgG2b kappa Log in to see GK1-5 9
0.3068567 ABIN2659719 FACS PE/Dazzle™ 594 Rat IgG2a kappa Log in to see RM4-5 8
0.3068567 ABIN2660762 FACS Biotin Rat IgG2b kappa Log in to see RM4-4 4
0.3068567 ABIN2661704 FACS IF IP IHC FITC Rat IgG2a kappa Log in to see H129-19 6

Top referenced anti-CD4 antibody for Flow Cytometry

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Application / Reactivity Mouse (Murine)
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Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 48 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 100 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 56 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Non-Polymorphic Site
(77), (22), (19), (13), (13), (13), (10), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(941), (591), (211), (60), (44), (25), (23), (22), (21), (15), (13), (9), (8), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rat
(1113), (549), (189), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to FITC
(254), (218), (150), (114), (37), (36), (24), (24), (21), (20), (19), (16), (15), (15), (14), (13), (9), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1383), (348), (330), (257), (208), (174), (159), (91), (72), (69), (33), (28), (27), (18), (14), (11), (10), (9), (7), (6), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118164) Images
Specificity This antibody reacts with the CD4 antigen, non polymorphic epitope. The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.
Purification Affinity Chromatography on Protein G.
Immunogen Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Clone YTS177-9
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN118164) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504
NCBI Accession NP_038516
UniProt P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN118164) Images back to top
Application Notes Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN118164) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 Antibody (ABIN118164)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Robinson, Graham, Emr: "A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation." in: Molecular and cellular biology, Vol. 11, Issue 12, pp. 5813-24, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118164) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (Non-Polymorphic Site) (FITC) (ABIN118164) Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD4:FITC