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anti-Mouse (Murine) CD4 antibody for Immunocytochemistry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
Extracellular
77
22
19
13
13
13
10
8
8
7
7
6
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
941
591
211
60
44
25
23
22
21
15
13
9
8
7
7
6
5
5
3
3
2
2
2
2
1
1
Host
Rat
1113
549
189
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
255
218
150
114
37
36
24
24
21
20
19
16
15
15
14
13
9
8
8
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
1383
348
330
256
208
173
159
90
72
68
33
28
27
18
14
11
10
9
7
6
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN343704
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009952392 ABIN509566 Func ICC FACS IHC (fro) IP FITC Rat IgG2b Extracellular Log in to see GK1-5 9
0.0009952392 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0009952392 ABIN2749008 Func IHC (f) ICC FACS IP Rat IgG2b Log in to see GK1-5 9
0.0009952392 ABIN2749027 Func IHC (f) ICC FACS IP Rat IgG2b Log in to see GK1-5 9
0.0009952392 ABIN1858300 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-394 Log in to see
0.0009952392 ABIN217742 CyTox ICC Rat IgG Log in to see
0.0009952392 ABIN1174282 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-394 Log in to see Polyclonal
0.0009952392 ABIN3199816 FACS Func ICC IHC (fro) IP APC Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN3199817 FACS Func ICC IHC (fro) IP FITC Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN3199818 FACS Func ICC IHC (fro) IP PE Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN3199819 FACS Func ICC IHC (fro) IP PerCP Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN2506618 ICC IF FITC Rabbit IgG Log in to see Polyclonal
0.0009952392 ABIN3199814 FACS Func ICC IHC (fro) IP Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN1112855 ICC FACS WB Rabbit IgG Log in to see Polyclonal
0.0009952392 ABIN3199813 FACS Func ICC IHC (fro) IP Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN3199815 FACS Func ICC IHC (fro) IP Rat IgG2b Log in to see GK1-5
0.0009952392 ABIN1854860 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
0.0009952392 ABIN2678873 ICC IHC (fro) LM eFluor® 570 Rat IgG2a kappa Log in to see RM4-5
0.0009952392 ABIN2678877 ICC IHC (fro) EFluor 615 Rat IgG2a kappa Log in to see RM4-5
0.0009952392 ABIN3037239 Func ICC FACS IF IP IHC PE Rat IgG2b Log in to see

Top referenced anti-CD4 antibody for Immunocytochemistry

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 48 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 100 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 56 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Extracellular
(77), (22), (19), (13), (13), (13), (10), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(941), (591), (211), (60), (44), (25), (23), (22), (21), (15), (13), (9), (8), (7), (7), (6), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rat
(1113), (549), (189), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(255), (218), (150), (114), (37), (36), (24), (24), (21), (20), (19), (16), (15), (15), (14), (13), (9), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(1383), (348), (330), (256), (208), (173), (159), (90), (72), (68), (33), (28), (27), (18), (14), (11), (10), (9), (7), (6), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN343704) Images
Specificity The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Mouse CTL clone V4 cells
Clone GK1-5
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN343704) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN343704) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1 µg/million cells
Immunoprecipitation: Recommended dilution: 1-2 µg/100-500 µg of protein in 1 mL lysate
Immunocytochemistry: Recommended dilution: 1-4 µg/mL
Functional Application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN343704) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD4 Antibody (ABIN343704)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Hu, Watson, Zhang, Graf, Wang, Sartor, Howden, Fletcher, Alexander: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, 2008

Yi, Zhen, Zeng, Zhang, Zhao: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." in: Transplantation, Vol. 85, Issue 8, pp. 1167-74, 2008

Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997

Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994

Godfrey, Kennedy, Gately, Hakimi, Hubbard, Zlotnik: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, 1994

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983

Background publications

Wilde, Marrack, Kappler, Dialynas, Fitch et al.: "Evidence implicating L3T4 in class II MHC antigen reactivity, monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2178-83, 1983