anti-Mouse (Murine) CD4 antibody for Intracellular Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1220
631
234
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1377
586
281
11
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to Alexa Fluor 488
266
227
170
103
49
48
45
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
1743
490
445
353
323
309
157
155
88
78
39
38
31
30
30
25
17
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2657424
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2660689 ICFC APC-Cy7 Rat IgG2b kappa Log in to see GK1-5 9
1 ABIN2661709 BR FACS IF ICFC IP IHC FITC Rat IgG2b kappa Log in to see RM4-4 4
1 ABIN2661703 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2657425 FACS ICFC Alexa Fluor 488 Rat IgG2b kappa Log in to see GK1-5 13
1 ABIN2661702 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 9
1 ABIN2661708 BR FACS IF ICFC IP IHC FITC Rat IgG2b kappa Log in to see RM4-4 4
1 ABIN2660690 ICFC APC-Cy7 Rat IgG2b kappa Log in to see GK1-5 10

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1220), (631), (234), (68), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1377), (586), (281), (11), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to Alexa Fluor 488
(266), (227), (170), (103), (49), (48), (45), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(1743), (490), (445), (353), (323), (309), (157), (155), (88), (78), (39), (38), (31), (30), (30), (25), (17), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN2657424) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone GK1-5
Isotype IgG2b kappa

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN2657424) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN2657424) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN2657424) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

ProductDetails: References for anti-CD4 antibody (ABIN2657424)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Inoue, Arikawa, Chen, Moriwaki, Price, Brown, Perfect, Shinohara: "T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 14, pp. 5295-300, 2014

Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997

Frei, Eugster, Bopst, Constantinescu, Lavi, Fontana: "Tumor necrosis factor alpha and lymphotoxin alpha are not required for induction of acute experimental autoimmune encephalomyelitis." in: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2177-82, 1997

Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989

Israelski, Araujo, Conley, Suzuki, Sharma, Remington: "Treatment with anti-L3T4 (CD4) monoclonal antibody reduces the inflammatory response in toxoplasmic encephalitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 3, pp. 954-8, 1989

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN2657424) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (Alexa Fluor 488) (ABIN2657424) anti-CD4 Molecule (CD4) antibody (Alexa Fluor 488)
Immunofluorescence (IF) image for anti-CD4 Molecule (CD4) antibody (Alexa Fluor 488) (ABIN2657424) anti-CD4 Molecule (CD4) antibody (Alexa Fluor 488) (Image 2)