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anti-Mouse (Murine) CD4 antibody for Immunofluorescence

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Human, Mouse (Murine)
1161
609
233
68
66
40
34
22
22
21
19
18
16
15
15
12
10
10
9
9
6
5
5
5
4
2
2
2
1
1
1
1
Host
Rabbit
1314
568
270
10
4
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
258
221
163
100
47
47
45
40
36
34
33
29
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1662
462
427
328
305
283
150
142
85
61
39
38
29
29
22
19
18
14
14
11
9
7
6
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN1532559
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.813965 ABIN439030 ICC IF IHC IHC (p) SimWes WB Rabbit C-Term, AA 400-458 Log in to see Polyclonal 11
11.813965 ABIN1531544 IF IHC ELISA Rabbit IgG pSer433 Log in to see Polyclonal
1 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 2
1 ABIN614792 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see GK1-5 7
1 ABIN2661072 FACS IF IHC Biotin Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2659512 FACS IF IHC PE-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN3183771 ELISA IF IHC WB Rabbit IgG Ser213 Log in to see Polyclonal
1 ABIN2661703 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2661704 FACS IF IP IHC FITC Rat IgG2a kappa Log in to see H129-19 6
1 ABIN2661709 BR FACS IF ICFC IP IHC FITC Rat IgG2b kappa Log in to see RM4-4 4
1 ABIN2660685 ELISA (Capture) IF IP WB APC-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2665991 FACS IF IHC Rat IgG2b kappa Log in to see GK1-5 9
1 ABIN2664669 FACS IF IHC Rat IgG2b kappa Log in to see GK1-5 9
1 ABIN498108 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN133255 FACS IF Biotin Rat IgG2b Log in to see YTS 191-1-2
1 ABIN133254 FACS IF Biotin Rat IgG2b Log in to see YTS 191-1-2
1 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN2851736 FACS IF PE-Cy5 Rat IgG2b Log in to see
1 ABIN2659513 FACS IF IHC PE-Cy7 Rat IgG2a kappa Log in to see RM4-5 9
1 ABIN2661702 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 9

Top referenced anti-CD4 antibody for Immunofluorescence

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 45 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 479 Antibodies
Functional Studies (Func) 22 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 124 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 67 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human, Mouse (Murine)
(1161), (609), (233), (68), (66), (40), (34), (22), (22), (21), (19), (18), (16), (15), (15), (12), (10), (10), (9), (9), (6), (5), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1314), (568), (270), (10), (4), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(258), (221), (163), (100), (47), (47), (45), (40), (36), (34), (33), (29), (27), (23), (21), (21), (19), (19), (18), (18), (18), (18), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1662), (462), (427), (328), (305), (283), (150), (142), (85), (61), (39), (38), (29), (29), (22), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN1532559) Images
Specificity CD4 Antibody detects endogenous levels of total CD4 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD4.
Isotype IgG

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN1532559) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Molecular weight of target protein: 51 kDa
Synonyms: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 precursor
Molecular Weight 51 kDa
Gene ID 920
OMIM 186940
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN1532559) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.631659 (NCBI Gene Symbol: CD4)

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN1532559) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CD4 Antibody (ABIN1532559)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Yamada, Yamaguchi, Asano, Fujita, Kobayashi, Kasai: "Th17-cells in atopic dermatitis stimulate orthodontic root resorption." in: Oral diseases, 2013 Method employed by authors: Immunofluorescence (IF) (1:100). Further details: For double immunofluorescence staining, the sections were deparaffinized and washed in TBS, and then were incubated with a polyclonal anti-rabbit CD4 antibody (Working dilution, 1:100) for 18 hours at 4°C.

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN1532559) back to top
Supplier Images
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Western blot analysis of extracts from COLO205 cells, using CD4 (Ab-433) Antibody. Th...
Immunohistochemistry (IHC) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD4 (Ab-...
Immunofluorescence (IF) image for anti-CD4 antibody (CD4 Molecule) (ABIN1532559) Immunofluorescence analysis of HeLa cells, using CD4 (Ab-433) Antibody. The picture o...