anti-Mouse (Murine) CD4 antibody for Immunofluorescence

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Human, Mouse (Murine)
1219
631
234
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rabbit
1377
587
280
11
4
1
1
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1744
489
444
353
322
309
157
154
88
78
39
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1532559
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.158281 ABIN439030 ICC IF IHC IHC (p) SimWes WB Rabbit C-Term, AA 400-458 Log in to see Polyclonal 12
11.158281 ABIN1531544 IF IHC ELISA Rabbit IgG pSer433 Log in to see Polyclonal
1 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 3
1 ABIN614792 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see GK1-5 7
1 ABIN2480261 IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN3183771 ELISA IF IHC WB Rabbit IgG Ser213 Log in to see Polyclonal
1 ABIN2660685 ELISA (Capture) IF IP WB APC-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2661704 FACS IF IP IHC FITC Rat IgG2a kappa Log in to see H129-19 6
1 ABIN2661709 BR FACS IF ICFC IP IHC FITC Rat IgG2b kappa Log in to see RM4-4 4
1 ABIN2661703 BR FACS IF ICFC IP IHC FITC Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2661072 FACS IF IHC Biotin Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2659512 FACS IF IHC PE-Cy7 Rat IgG2a kappa Log in to see RM4-5 8
1 ABIN2665991 FACS IF IHC Rat IgG2b kappa Log in to see GK1-5 9
1 ABIN2664669 FACS IF IHC Rat IgG2b kappa Log in to see GK1-5 9
1 ABIN498108 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN133255 FACS IF Biotin Rat IgG2b Log in to see YTS 191-1-2
1 ABIN133254 FACS IF Biotin Rat IgG2b Log in to see YTS 191-1-2
1 ABIN2480287 Func IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human, Mouse (Murine)
(1219), (631), (234), (68), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1377), (587), (280), (11), (4), (1), (1), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1744), (489), (444), (353), (322), (309), (157), (154), (88), (78), (39), (38), (31), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images
Specificity CD4 Antibody detects endogenous levels of total CD4 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD4.
Isotype IgG

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Molecular weight of target protein: 51 kDa
Synonyms: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 precursor
Molecular Weight 51 kDa
Gene ID 920
OMIM 186940
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.631659 (NCBI Gene Symbol: CD4)

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN1532559) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CD4 antibody (ABIN1532559)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Yamada, Yamaguchi, Asano, Fujita, Kobayashi, Kasai: "Th17-cells in atopic dermatitis stimulate orthodontic root resorption." in: Oral diseases, 2013 Method employed by authors: Immunofluorescence (IF) (1:100). Further details: For double immunofluorescence staining, the sections were deparaffinized and washed in TBS, and then were incubated with a polyclonal anti-rabbit CD4 antibody (Working dilution, 1:100) for 18 hours at 4°C.

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN1532559) back to top
Supplier Images
Western Blotting (WB) image for anti-CD4 Molecule (CD4) antibody (ABIN1532559) Western blot analysis of extracts from COLO205 cells, using CD4 (Ab-433) Antibody. Th...
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) antibody (ABIN1532559) Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CD4 (Ab-...
Immunofluorescence (IF) image for anti-CD4 Molecule (CD4) antibody (ABIN1532559) Immunofluorescence analysis of HeLa cells, using CD4 (Ab-433) Antibody. The picture o...