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anti-Mouse (Murine) CD4 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
AA 420-457
77
40
22
22
13
13
12
10
8
8
7
7
7
7
6
6
6
5
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1197
690
239
66
52
45
25
23
22
21
19
15
14
9
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rabbit
1359
664
275
4
2
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
282
237
175
114
44
42
42
39
39
38
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1803
435
395
352
309
297
179
135
100
70
32
28
28
27
22
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN671376
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088330376 ABIN672936 IF (p) IHC (p) WB Rabbit IgG AA 230-280 Log in to see Polyclonal 3
0.00088330376 ABIN889090 IF (p) Alexa Fluor 555 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN889092 IF (p) Alexa Fluor 350 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN889094 IF (p) Alexa Fluor 555 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN889093 IF (p) Alexa Fluor 488 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN889099 IF (p) Alexa Fluor 350 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN889095 IF (p) Alexa Fluor 647 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN889098 IF (p) Alexa Fluor 488 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN889097 IF (p) Alexa Fluor 647 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN671381 IF (p) Cy5.5 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN671380 IF (p) Cy5 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN671379 IF (p) Cy3 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN671383 IF (p) FITC Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN671382 IF (p) Cy7 Rabbit IgG AA 420-457 Log in to see Polyclonal
0.00088330376 ABIN672942 IF (p) Cy7 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN672943 IF (p) FITC Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN672939 IF (p) Cy3 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN672940 IF (p) Cy5 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN672941 IF (p) Cy5.5 Rabbit IgG AA 230-280 Log in to see Polyclonal
0.00088330376 ABIN2811349 IF (p) Alexa Fluor 594 Rabbit IgG AA 230-280 Log in to see Polyclonal

Top referenced anti-CD4 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
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Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 174 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 9 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Neutralization (Neut) 1 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope AA 420-457
(77), (40), (22), (22), (13), (13), (12), (10), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1197), (690), (239), (66), (52), (45), (25), (23), (22), (21), (19), (15), (14), (9), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1359), (664), (275), (4), (2), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(282), (237), (175), (114), (44), (42), (42), (39), (39), (38), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1803), (435), (395), (352), (309), (297), (179), (135), (100), (70), (32), (28), (28), (27), (22), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN671376) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat CD4
Isotype IgG

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN671376) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Subcellular location: Cytoplasm


Synonyms: p55, W3/25, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4
Gene ID 24932
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN671376) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN671376) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CD4 Antibody (ABIN671376)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, 2015

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN671376) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclona...
Immunofluorescence (IF) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibo...
Immunohistochemistry (IHC) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgica...
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated fo...
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (AA 420-457) (ABIN671376) Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min...
'Independent Validation' Badge
Lot Number 130703
Method validated Immunohistochemistry
Positive Control Mouse spleen tissue
Negative Control Mouse muscle tissue
Notes Signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antibody: CD4 Molecule (CD4)
  • Catalog number: ABIN671376
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130703
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Spleen tissue (specimen known to contain the target protein) from mouse.
  • Negative Control: Muslce tissue (specimen known to not contain the target protein) from mouse.
  • Primary antibody isotype control: Mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Mouse spleen treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 20 sec.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Experimental Notes - No experimental challenges noted.
Validation Images
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 1: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 2: Mouse muscle stained with anti-CD4 (brown) and counterstained with hematoxy...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 3: Mouse spleen stained with isotype control antibody (brown) and counterstain...
Immunohistochemistry validation image for anti-CD4 Molecule (CD4) (AA 420-457) antibody (ABIN671376) Figure 4: Mouse spleen stained with secondary antibody only (brown) and counterstaine...