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|Application / Reactivity||Rabbit|
|Blocking Reagent (BR)||1 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Simple Western (SimWes)||2 Antibodies|
|Western Blotting (WB)||4 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Conjugate||This CD4 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
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Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling Images
|Specificity||This antibody recognises the CD4 cell surface antigen, a 50 kDa glycoprotein expressed by helper T lymphocytes.|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Rabbit thymocytes. Spleen cells from immunised mice were fused with cells of the mouse PAI myeloma cell line|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling Images back to top
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details Images back to top
|Buffer||PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-CD4 Antibody Target Details CD4 Application Details Handling back to top