anti-Rat (Rattus) CD4 antibody for Functional Studies

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
Domain 1
66
64
20
19
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1220
632
233
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1376
587
281
11
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1743
490
445
352
323
309
156
155
88
77
39
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN120377
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120378 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN111996 Func FACS IHC (fro) Mouse IgG2a Log in to see Rib 5-2 2
1 ABIN120376 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN120372 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain 1
(66), (64), (20), (19), (15), (15), (15), (13), (12), (10), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1220), (632), (233), (68), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1376), (587), (281), (11), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(1743), (490), (445), (352), (323), (309), (156), (155), (88), (77), (39), (38), (31), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120377) Images
Purification Affinity Chromatography on Protein A
Immunogen Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Clone W3-25
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120377) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN120377) Images back to top
Application Notes Flow Cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN120377) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD4 antibody (ABIN120377)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Schwartzkopff, Schlereth, Berger, Bredow, Birnbaum, Böhringer, Reinhard: "NK cell depletion delays corneal allograft rejection in baby rats." in: Molecular vision, Vol. 16, pp. 1928-35, 2010

Zilka, Stozicka, Kovac, Pilipcinec, Bugos, Novak: "Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy." in: Journal of neuroimmunology, Vol. 209, Issue 1-2, pp. 16-25, 2009

Hofmann, Lachnit, Streppel, Witter, Neiss, Guntinas-Lichius, Angelov: "Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats." in: BMC immunology, Vol. 3, pp. 11, 2003

Banerjee, Figueiredo, Easty, Dick, Nicholls: "Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats." in: The British journal of ophthalmology, Vol. 87, Issue 12, pp. 1515-22, 2003

Bjersing, Telemo, Dahlgren, Hanson: "Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats." in: Clinical and experimental immunology, Vol. 130, Issue 3, pp. 404-8, 2002

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Pelegrí, Franch, Castellote, Castell: "Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis." in: The Journal of rheumatology, Vol. 22, Issue 1, pp. 124-32, 1995

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Williams, Galfrè, Milstein: "Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes." in: Cell, Vol. 12, Issue 3, pp. 663-73, 1978

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120377) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD4 Molecule (CD4) (Domain 1) antibody (ABIN120377) Staining of frozen rat spleen with Mouse anti rat CD4