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anti-Rat (Rattus) CD4 antibody for Immunofluorescence

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Epitope
C-Term, AA 400-458
77
40
22
16
13
13
13
9
8
8
7
7
7
6
6
6
5
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
1197
694
240
72
52
45
25
23
22
21
19
15
15
14
10
9
8
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rabbit
1372
670
267
4
2
1
Clonality
Polyclonal
Conjugate
This CD4 antibody is un-conjugated
286
239
178
113
44
43
42
39
39
38
35
33
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
1816
434
399
366
307
303
180
140
103
70
33
29
28
27
23
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN439030
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010988911 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0010988911 ABIN1585961 IF FITC Rabbit C-Term Log in to see Polyclonal
0.0010988911 ABIN1586035 IF FITC Rabbit Middle Region Log in to see Polyclonal
0.0010988911 ABIN2480233 IHC (fro) FACS IF Mouse IgG2a Domain 2 Log in to see OX-35 4
0.0010988911 ABIN2506618 ICC IF FITC Rabbit IgG Log in to see Polyclonal
0.0010988911 ABIN4292746 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-458, C-Term Log in to see Polyclonal
0.0010988911 ABIN1854860 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
0.0010988911 ABIN3037243 ICC FACS IF IHC Mouse IgG2a Log in to see
0.0010988911 ABIN3037246 ICC FACS IF IHC APC Mouse IgG2a Log in to see
0.0010988911 ABIN3037244 ICC FACS IF IHC FITC Mouse IgG2a Log in to see
0.0010988911 ABIN3037245 ICC FACS IF IHC PE Mouse IgG2a Log in to see
0.0010988911 ABIN1854063 ICC FACS IF IP FITC Mouse IgG2a Log in to see
0.0010988911 ABIN1854065 ICC FACS IF IP PE Mouse IgG2a Log in to see
0.0010988911 ABIN1854064 ICC FACS IF IP Biotin Mouse IgG2a Log in to see

Top referenced anti-CD4 antibody for Immunofluorescence

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 174 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 9 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Neutralization (Neut) 1 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope C-Term, AA 400-458
(77), (40), (22), (16), (13), (13), (13), (9), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(1197), (694), (240), (72), (52), (45), (25), (23), (22), (21), (19), (15), (15), (14), (10), (9), (8), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1372), (670), (267), (4), (2), (1)
Clonality
Polyclonal
Conjugate This CD4 antibody is un-conjugated
(286), (239), (178), (113), (44), (43), (42), (39), (39), (38), (35), (33), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(1816), (434), (399), (366), (307), (303), (180), (140), (103), (70), (33), (29), (28), (27), (23), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN439030) Images
Cross-Reactivity (Details) Rabbit reactivity reported in the scientific literature (PMID: 22127304).
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an C-terminal region of the human CD4 protein (within residues 400-458). [Swiss-Prot P01730]

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN439030) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Gene Symbol: CD4
Gene ID 920, 12504
UniProt P01730, P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN439030) Images back to top
Application Notes Western Blot 1 μg/mL, Simple Western 1:100, Immunohistochemistry 1:50-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for CD4 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Protocol Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN439030) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD4 Antibody (ABIN439030)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Song, Endo, Song, Shibata, Koji: "5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases." in: Toxicology, Vol. 361-362, pp. 62-72, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Ren, Li, Tian, Nie, Wang, Li, Zheng: "Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice." in: Scientific reports, Vol. 5, pp. 15730, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Gervais, Desantis, Pagendarm, Nelson, Enger, Skarstein, Liaaen Jensen, Larsen: "Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 298, Issue 9, pp. 1622-34, 2015 Method employed by authors: Chromatin Immunoprecipitation(ChIP)

Yang, Vethanayagam, Dong, Cai, Hu: "Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation." in: Neuroscience, Vol. 303, pp. 1-15, 2015 Method employed by authors: Flow Cytometry(FACS)

Migita, Sho, Shimada, Yasuda, Yamato, Takayama, Matsumoto, Wakatsuki, Hotta, Tanaka, Ito, Konishi, Nakajima: "Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma." in: Cancer, Vol. 120, Issue 6, pp. 808-17, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Helsby, Leader, Fenn, Gulsen, Bryant, Doughton, Sharpe, Whitley, Caunt, James, Pope, Kelly, Chalmers: "CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited." in: BMC cell biology, Vol. 15, pp. 6, 2014 (Sample species: Human).

Yasuda, Sho, Yamato, Yoshiji, Wakatsuki, Nishiwada, Yagita, Nakajima: "Simultaneous blockade of programmed death 1 and vascular endothelial growth factor receptor 2 (VEGFR2) induces synergistic anti-tumour effect in vivo." in: Clinical and experimental immunology, Vol. 172, Issue 3, pp. 500-6, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Zheng, Li, Tian, Ren, Du, Li: "Notch γ-secretase inhibitor dibenzazepine attenuates angiotensin II-induced abdominal aortic aneurysm in ApoE knockout mice by multiple mechanisms." in: PLoS ONE, Vol. 8, Issue 12, pp. e83310, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Hwang, Ahn, Park, Ha, Song, Jee: "An acidic polysaccharide of Panax ginseng ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells." in: Immunology letters, Vol. 138, Issue 2, pp. 169-78, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Lu, Li, Wang, Zheng, Pu, Ding, Li, Xu, Gao, Chen: "The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits." in: Cryobiology, Vol. 64, Issue 1, pp. 27-32, 2011 (Sample species: Rabbit). Further details: Immunohistochemistry

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN439030) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) anti-CD4 Molecule (CD4) (AA 400-458), (C-Term) antibody
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) anti-CD4 Molecule (CD4) (AA 400-458), (C-Term) antibody (Image 2)
Simple Western (SimWes) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Simple Western: CD4 Antibody [NBP1-19371] - Simple Western lane view shows a specific...
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following ce...
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image fro...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin...
Western Blotting (WB) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Western Blot: CD4 Antibody [NBP1-19371] - Dog spleen homogenate. Probed with anti-CD4...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embed...
Immunofluorescence (IF) image for anti-CD4 antibody (CD4 Molecule) (C-Term) (ABIN439030) Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was ...