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anti-Cow (Bovine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Cow (Bovine), Goat, Sheep (Ovine)
1059
532
167
68
47
40
25
22
19
19
19
16
15
13
12
12
11
11
10
9
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
258
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1617
452
356
343
285
282
255
238
214
37
33
25
23
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN317440
$ 753.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN2472467 FACS PE Mouse IgG2a Log in to see CC63 4
0.30464333 ABIN2169197 FACS PE Mouse IgG2a Log in to see
0.30464333 ABIN2472510 FACS PE Mouse IgG2a Log in to see 38-65 4
0.30464333 ABIN2166830 FACS FITC Mouse IgG2a Log in to see
0.0010463598 ABIN317442 FACS PE Mouse IgG2a Log in to see CC63 5
0.0010463598 ABIN1449140 IHC (fro) FACS IF IHC (p) IP Mouse IgG2a Log in to see CC63 7
0.0010463598 ABIN2472465 FACS Alexa Fluor 647 Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN2472466 FACS FITC Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN1820268 FACS PE Mouse IgG2a Log in to see CC63
0.0010463598 ABIN1820267 FACS FITC Mouse IgG2a Log in to see CC63
0.0010463598 ABIN2228800 FACS PE Mouse IgG2a Log in to see 8H5
0.0010463598 ABIN2472509 FACS FITC Mouse IgG2a Log in to see 38-65 4
0.0010463598 ABIN2472464 IHC (fro) FACS IF IP IHC (p) Mouse IgG2a Log in to see CC63 4
0.0010463598 ABIN2228763 FACS IF IP IHC Mouse IgG2a Log in to see
0.0010463598 ABIN2228801 FACS FITC Mouse IgG2a Log in to see 8H5
0.0010463598 ABIN2472508 IHC (fro) FACS IP Mouse IgG2a Log in to see 38-65 4
0.0010463598 ABIN1819209 IHC (fro) FACS IF IHC (p) IP Mouse IgG2a Log in to see CC63
0.0010463598 ABIN641715 FACS PE Mouse IgG2a Log in to see
0.0010463598 ABIN641714 FACS FITC Mouse Log in to see

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Sheep (Ovine) Cow (Bovine) Goat
Immunoprecipitation (IP) 4 Antibodies 5 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 10 Antibodies 19 Antibodies 20 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Cow (Bovine), Goat, Sheep (Ovine)
(1059), (532), (167), (68), (47), (40), (25), (22), (19), (19), (19), (16), (15), (13), (12), (12), (11), (11), (10), (9), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1285), (512), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(258), (217), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1617), (452), (356), (343), (285), (282), (255), (238), (214), (37), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN317440) Images
Specificity This antibody reacts with the CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34 kDa and 34 kDa under reducing conditions.
Characteristics Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Clone CC63
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN317440) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 281060
UniProt P31783
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN317440) Images back to top
Application Notes Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN317440) Images back to top
Concentration 0.1 mg/mL
Buffer Phosphate buffered saline, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. This antibody is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD8 Antibody (ABIN317440)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Winkler, Doster, Jones: "Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves." in: Journal of virology, Vol. 74, Issue 11, pp. 5337-46, 2000

Twizere, Kerkhofs, Burny, Portetelle, Kettmann, Willems: "Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo." in: Journal of virology, Vol. 74, Issue 21, pp. 9895-902, 2000

Gutierrez, Forster, McConnell, Cassidy, Pollock, Bryson: "The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques." in: Veterinary immunology and immunopathology, Vol. 71, Issue 3-4, pp. 321-34, 1999

Winkler, Doster, Jones: "Bovine herpesvirus 1 can infect CD4(+) T lymphocytes and induce programmed cell death during acute infection of cattle." in: Journal of virology, Vol. 73, Issue 10, pp. 8657-68, 1999

MacHugh, Sopp: "Individual antigens of cattle. Bovine CD8 (BoCD8)." in: Veterinary immunology and immunopathology, Vol. 27, Issue 1-3, pp. 65-9, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN317440) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN317440) anti-CD8a Molecule (CD8A) antibody (FITC)