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|Application / Reactivity||Dog (Canine)|
|Flow Cytometry (FACS)||21 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Dog (Canine) Alternatives|
|Conjugate||This CD8 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS)
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Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling Images
|Specificity||This antibody, clone YCATE 55.9 was clustered as CD8 in the first canine leukocyte antigen workshop. The antibody reacts with canine CD8a transfectants. Clone YCATE 55.9 is reported to block MHC class I dependant T-cell responses in vitro and in vivo.|
|Characteristics||Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Canine CD8a chimaeric human IgG1 Fc fusion protein. Spleen cells from immunised DA ratwere fused with cells of the Y3/A1. 2. 3 rat myeloma cell line.|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
|Expiry Date||12 months|
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