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anti-Dog (Canine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Dog (Canine)
1026
538
164
73
47
41
22
21
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rat
1277
509
193
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
257
217
138
81
56
44
41
37
33
29
28
28
26
25
24
24
24
24
20
20
19
17
16
14
12
11
10
10
10
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1617
439
351
337
269
262
240
235
199
39
37
25
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN182025
$ 731.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28286862 ABIN2472516 FACS PE Rat IgG1 Log in to see YCATE55-9 4
0.28286862 ABIN2168253 FACS PE Rat IgG1 Log in to see
0.28286862 ABIN2167364 FACS FITC Rat IgG1 Log in to see
0.0010565212 ABIN182027 FACS PE Rat IgG1 Log in to see YCATE55-9
0.0010565212 ABIN181842 FACS IHC (fro) IP Mouse IgG2a Log in to see CA9-JD3 2
0.0010565212 ABIN2228862 FACS PE Rat IgG1 Log in to see 10B2000
0.0010565212 ABIN2472514 FACS FITC Rat IgG1 Log in to see YCATE55-9 4
0.0010565212 ABIN2228861 FACS FITC Rat IgG1 Log in to see 10B2000
0.0010565212 ABIN2472512 FACS Alexa Fluor 647 Rat IgG1 Log in to see YCATE55-9 4
0.0010565212 ABIN2472513 FACS Alexa Fluor 700 Rat IgG1 Log in to see YCATE55-9 4
0.0010565212 ABIN2472515 FACS Pacific Blue Rat IgG1 Log in to see YCATE55-9 4
0.0010565212 ABIN2229199 FACS IP IHC Mouse IgG2a Log in to see 10B2024
0.0010565212 ABIN2229309 FACS IHC Rat IgG1 Log in to see 3H889
0.0010565212 ABIN2472528 IHC (fro) FACS IP Mouse IgG2a Log in to see CA9-JD3 4
0.0010565212 ABIN2472511 IHC (fro) FACS IF Rat IgG1 Log in to see YCATE55-9 4
0.0010565212 ABIN2228764 FACS IF IHC Rat IgG1 Log in to see
0.0010565212 ABIN2166831 IHC (af) FACS IP Mouse IgG2a Log in to see
0.0010565212 ABIN642158 FACS PE Rat Log in to see
0.0010565212 ABIN642157 FACS FITC Rat Log in to see
0.0010565212 ABIN642905 IHC (fro) FACS IP Mouse Log in to see

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Dog (Canine)
Flow Cytometry (FACS) 21 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunoprecipitation (IP) 5 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Dog (Canine)
(1026), (538), (164), (73), (47), (41), (22), (21), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1277), (509), (193), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(257), (217), (138), (81), (56), (44), (41), (37), (33), (29), (28), (28), (26), (25), (24), (24), (24), (24), (20), (20), (19), (17), (16), (14), (12), (11), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1617), (439), (351), (337), (269), (262), (240), (235), (199), (39), (37), (25), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Specificity This antibody, clone YCATE 55.9 was clustered as CD8 in the first canine leukocyte antigen workshop. The antibody reacts with canine CD8a transfectants. Clone YCATE 55.9 is reported to block MHC class I dependant T-cell responses in vitro and in vivo.
Characteristics Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Purification Affinity chromatography on Protein G
Immunogen Canine CD8a chimaeric human IgG1 Fc fusion protein. Spleen cells from immunised DA ratwere fused with cells of the Y3/A1. 2. 3 rat myeloma cell line.
Clone YCATE55-9
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 403157
UniProt P33706
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN182025) Staining of canine peripheral blood lymphocytes with RAT ANTI CANINE CD8:FITC (ABIN18...