We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Co-Stimulation (CoSt)||2 Antibodies|
|Cytotoxicity Test (CyTox)||7 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||559 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||5 Antibodies|
|Immunochromatography (IC)||10 Antibodies|
|Immunocytochemistry (ICC)||42 Antibodies|
|Immunofluorescence (IF)||59 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||146 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||10 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||2 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Conjugate||This CD8 antibody is conjugated to FITC Alternatives|
Blocking Reagent (BR), Co-Stimulation (CoSt), Flow Cytometry (FACS), Immunoprecipitation (IP)
|7 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2661824) Images
|Cross-Reactivity||Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops), Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus Monkey, Sooty mangabey (Cercocebus atys), Green Monkey (Chlorocebus sabaeus)|
|Purification||The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2661824) Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2661824) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2661824) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD8 Antibody (ABIN2661824)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Chen, Tsau, Molkentin et al.: "Mechanisms of necroptosis in T cells." in: The Journal of experimental medicine, Vol. 208, Issue 4, pp. 633-41, 2011 (PubMed).
Wooldridge, van den Berg, Glick et al.: "Interaction between the CD8 coreceptor and major histocompatibility complex class I stabilizes T cell receptor-antigen complexes at the cell surface." in: The Journal of biological chemistry, Vol. 280, Issue 30, pp. 27491-501, 2005 (PubMed).
Campanelli, Palermo, Garbelli et al.: "Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation." in: International immunology, Vol. 14, Issue 1, pp. 39-44, 2001 (PubMed).
Ahmed, Ohtani, Nio et al.: "CD8+ T cells infiltrating into bile ducts in biliary atresia do not appear to function as cytotoxic T cells: a clinicopathological analysis." in: The Journal of pathology, Vol. 193, Issue 3, pp. 383-9, 2001 (PubMed).
Carbone, Gloghini, Larocca et al.: "Human immunodeficiency virus-associated Hodgkin's disease derives from post-germinal center B cells." in: Blood, Vol. 93, Issue 7, pp. 2319-26, 1999 (PubMed).
Evans, Wall, Platsoucas et al.: "Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 1, pp. 544-8, 1981 (PubMed).
Ledbetter, Evans, Lipinski et al.: "Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man." in: The Journal of experimental medicine, Vol. 153, Issue 2, pp. 310-23, 1981 (PubMed).
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2661824) back to top