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anti-Human CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1063
556
167
73
47
41
25
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1319
536
188
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC
263
223
144
86
56
44
44
40
33
30
30
29
28
25
24
24
24
24
20
20
19
18
17
14
11
10
10
10
9
8
6
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1656
446
353
338
269
261
244
235
199
41
33
25
23
17
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN181874
€ 487.14
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.291056 ABIN119507 FACS FITC Mouse IgG1 Log in to see LT8 5
0.291056 ABIN119510 FACS PE Mouse IgG1 Log in to see LT8 1
0.291056 ABIN119442 FACS FITC Rat IgG2b Log in to see YTC 182-20 1
0.291056 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
0.291056 ABIN969474 FACS ELISA Mouse IgG1 Log in to see RAVB3 1
0.291056 ABIN119506 FACS Biotin Mouse IgG1 Log in to see LT8
0.291056 ABIN1106581 FACS PE Mouse IgG2a Log in to see B-Z31
0.291056 ABIN2658082 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T8 7
0.291056 ABIN2661146 FACS Biotin Mouse IgG1 kappa Log in to see HIT8a 3
0.291056 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
0.291056 ABIN2658080 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see HIT8a 3
0.291056 ABIN2656837 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see RPA-T8 5
0.291056 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.291056 ABIN2657187 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see SK1 7
0.291056 ABIN2657270 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see SK1 7
0.291056 ABIN2657486 FACS IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see HIT8a 3
0.291056 ABIN2660420 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see HIT8a 3
0.291056 ABIN2659552 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see SK1 7
0.291056 ABIN2659562 FACS IHC ELISA WB PE-Cy7 Mouse IgG1 kappa Log in to see HIT8a 3
0.291056 ABIN2659564 FACS PE-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Flow Cytometry

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General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1063), (556), (167), (73), (47), (41), (25), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1319), (536), (188), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC
(263), (223), (144), (86), (56), (44), (44), (40), (33), (30), (30), (29), (28), (25), (24), (24), (24), (24), (20), (20), (19), (18), (17), (14), (11), (10), (10), (10), (9), (8), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1656), (446), (353), (338), (269), (261), (244), (235), (199), (41), (33), (25), (23), (17), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images
Specificity This antibody recognises the alpha-chain of the CD8 antigen, a 32 kDa glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
Purification Ion Exchange Chromatography
Immunogen Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human wholeblood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN181874) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20 °C.

References for anti-CD8 Antibody (ABIN181874)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (APC) (ABIN181874) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8:APC (ABIN181...