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anti-Human CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1070
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1304
519
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
262
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1640
470
373
351
290
285
264
246
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN119507
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3087334 ABIN181874 FACS APC Mouse IgG1 Log in to see LT8 1
0.3087334 ABIN119442 FACS FITC Rat IgG2b Log in to see YTC 182-20 1
0.3087334 ABIN119510 FACS PE Mouse IgG1 Log in to see LT8 1
0.3087334 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
0.3087334 ABIN94233 FACS IP Biotin Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN969474 FACS ELISA Mouse IgG1 Log in to see RAVB3 1
0.3087334 ABIN119506 FACS Biotin Mouse IgG1 Log in to see LT8
0.3087334 ABIN1106581 FACS PE Mouse IgG2a Log in to see B-Z31
0.3087334 ABIN94235 FACS IP PE Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN302050 FACS PerCP Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN94234 FACS FITC Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN94232 FACS IP Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN94236 FACS APC Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN192069 FACS IP PE-DyLight 594 Mouse IgG2a Log in to see MEM-31 7
0.3087334 ABIN2656837 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see RPA-T8 5
0.3087334 ABIN2658080 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see HIT8a 3
0.3087334 ABIN2658082 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T8 7
0.3087334 ABIN2661146 FACS Biotin Mouse IgG1 kappa Log in to see HIT8a 3
0.3087334 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
0.3087334 ABIN1106564 FACS IHC (p) Mouse IgG2a Log in to see B-Z31

Top referenced anti-CD8 antibody for Flow Cytometry

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General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1070), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1304), (519), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(262), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1640), (470), (373), (351), (290), (285), (264), (246), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN119507) Images
Specificity This antibody recognises the CD8 cell surface glycoprotein.
Purification Ion Exchange Chromatography.
Immunogen Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN119507) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN119507) Images back to top
Application Notes Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN119507) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN119507)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Nelson, Lever, Dean, Savage, Salguero, Pearce, Stevens, Simpson: "Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus)." in: Journal of medical microbiology, Vol. 59, Issue Pt 9, pp. 1107-13, 2010

Kap, van Meurs, van Driel, Koopman, Melief, Brok, Laman, t Hart: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009

Gibbings, Marcet-Palacios, Sekar, Ng, Befus: "CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses." in: BMC immunology, Vol. 8, pp. 12, 2007

Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN119507) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN119507) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8:FITC (ABIN11...