You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
1056
551
165
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1287
523
221
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC
264
220
141
80
58
46
43
39
35
31
29
28
27
26
26
26
26
25
21
21
19
17
16
15
11
10
10
10
9
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1651
466
447
319
271
269
267
246
231
39
38
25
25
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN181874
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.113617 ABIN119507 FACS FITC Mouse IgG1 Log in to see LT8 5
13.113617 ABIN119510 FACS PE Mouse IgG1 Log in to see LT8 1
13.113617 ABIN119442 FACS FITC Rat IgG2b Log in to see YTC 182-20 1
13.113617 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
13.113617 ABIN1385272 FACS IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 1
13.113617 ABIN4295988 FACS IHC (fro) in vitro Mouse IgG1 kappa Log in to see RPA-T8 7
13.113617 ABIN969474 FACS ELISA Mouse IgG1 Log in to see RAVB3 1
13.113617 ABIN4896426 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
13.113617 ABIN4896431 FACS PE Mouse IgG2b AA 22-198 Log in to see 37006 1
13.113617 ABIN4896432 FACS PE Mouse IgG2b AA 22-198 Log in to see 37006 1
13.113617 ABIN4896427 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
13.113617 ABIN4262030 FACS PE Mouse IgG1 kappa Log in to see RPA-T8 1
13.113617 ABIN119506 FACS Biotin Mouse IgG1 Log in to see LT8
13.113617 ABIN1106581 FACS PE Mouse IgG2a Log in to see B-Z31
13.113617 ABIN2656837 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see RPA-T8 5
13.113617 ABIN2658082 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T8 7
13.113617 ABIN2658080 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see HIT8a 3
13.113617 ABIN2661146 FACS Biotin Mouse IgG1 kappa Log in to see HIT8a 3
13.113617 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
13.113617 ABIN1106564 FACS IHC (p) Mouse IgG2a Log in to see B-Z31

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 17 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 199 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 803 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 161 Antibodies
Immunofluorescence (IF) 180 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 222 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1056), (551), (165), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1287), (523), (221), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC
(264), (220), (141), (80), (58), (46), (43), (39), (35), (31), (29), (28), (27), (26), (26), (26), (26), (25), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1651), (466), (447), (319), (271), (269), (267), (246), (231), (39), (38), (25), (25), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images
Specificity This antibody recognises the alpha-chain of the CD8 antigen, a 32 kDa glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
Purification Ion Exchange Chromatography
Immunogen Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human wholeblood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN181874) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20 °C.

References for anti-CD8 Antibody (ABIN181874)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (APC) (ABIN181874) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8:APC (ABIN181...