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anti-Human CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human
1050
536
161
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1283
510
215
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC
261
219
141
80
57
45
42
38
34
30
29
28
26
26
25
25
25
24
21
21
19
17
16
15
11
10
10
10
9
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1626
452
449
316
271
267
261
246
234
39
33
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN181874
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.7474785 ABIN119507 FACS FITC Mouse IgG1 Log in to see LT8 5
13.7474785 ABIN119442 FACS FITC Rat IgG2b Log in to see YTC 182-20 1
13.7474785 ABIN119510 FACS PE Mouse IgG1 Log in to see LT8 1
13.7474785 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
13.7474785 ABIN969474 FACS ELISA Mouse IgG1 Log in to see RAVB3 1
13.7474785 ABIN4896427 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
13.7474785 ABIN4896426 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
13.7474785 ABIN4896432 FACS PE Mouse IgG2b AA 22-198 Log in to see 37006 1
13.7474785 ABIN1385272 FACS IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 1
13.7474785 ABIN4295988 FACS IHC (fro) in vitro Mouse IgG1 kappa Log in to see RPA-T8 7
13.7474785 ABIN119506 FACS Biotin Mouse IgG1 Log in to see LT8
13.7474785 ABIN1106581 FACS PE Mouse IgG2a Log in to see B-Z31
13.7474785 ABIN4262030 FACS PE Mouse IgG1 kappa Log in to see RPA-T8 1
13.7474785 ABIN2661146 FACS Biotin Mouse IgG1 kappa Log in to see HIT8a 3
13.7474785 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
13.7474785 ABIN2658080 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see HIT8a 3
13.7474785 ABIN2658082 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T8 7
13.7474785 ABIN2656837 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see RPA-T8 5
13.7474785 ABIN192069 FACS IP PE-DyLight 594 Mouse IgG2a Log in to see MEM-31 7
13.7474785 ABIN94232 FACS IP Mouse IgG2a Log in to see MEM-31 7

Top referenced anti-CD8 antibody for Flow Cytometry

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General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1050), (536), (161), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1283), (510), (215), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC
(261), (219), (141), (80), (57), (45), (42), (38), (34), (30), (29), (28), (26), (26), (25), (25), (25), (24), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1626), (452), (449), (316), (271), (267), (261), (246), (234), (39), (33), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images
Specificity This antibody recognises the alpha-chain of the CD8 antigen, a 32 kDa glycoprotein expressed by the cytotoxicsuppressor subset of T cells and weakly by NK cells.
Purification Ion Exchange Chromatography
Immunogen Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN181874) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human wholeblood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN181874) Images back to top
Reconstitution Restore with 1 mL distilled water
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20 °C.

References for anti-CD8 Antibody (ABIN181874)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN181874) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (APC) (ABIN181874) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8:APC (ABIN181...