anti-Human CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human, Mouse (Murine)
599
495
127
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
764
458
198
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
150
143
91
55
45
36
36
33
29
27
24
23
23
23
23
22
20
19
19
18
14
13
11
11
11
10
9
7
7
7
5
4
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
1149
445
364
256
246
236
197
185
173
35
34
20
16
13
11
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN268292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.320037 ABIN4295988 FACS IHC (fro) in vitro Mouse IgG1 kappa Log in to see RPA-T8 8
14.320037 ABIN4896427 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
14.320037 ABIN4896426 FACS Fluorescein Mouse IgG2b AA 22-198 Log in to see 37006 1
14.320037 ABIN4896431 FACS PE Mouse IgG2b AA 22-198 Log in to see 37006 1
14.320037 ABIN4896432 FACS PE Mouse IgG2b AA 22-198 Log in to see 37006 1
14.320037 ABIN4262030 FACS PE Mouse IgG1 kappa Log in to see RPA-T8 1
14.320037 ABIN2656837 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see RPA-T8 5
14.320037 ABIN2661146 FACS Biotin Mouse IgG1 kappa Log in to see HIT8a 3
14.320037 ABIN2661147 FACS WB Biotin Mouse IgG1 kappa Log in to see RPA-T8 6
14.320037 ABIN2658080 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see HIT8a 3
14.320037 ABIN2658082 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see RPA-T8 7
14.320037 ABIN192069 FACS IP PE-DyLight 594 Mouse IgG2a Log in to see MEM-31 7
14.320037 ABIN94232 FACS IP Mouse IgG2a Log in to see MEM-31 7
14.320037 ABIN94234 FACS IP FITC Mouse IgG2a Log in to see MEM-31 7
14.320037 ABIN94236 FACS IP APC Mouse IgG2a Log in to see MEM-31 7
14.320037 ABIN2659020 FACS PE-Cy5 Mouse IgG1 kappa Log in to see RPA-T8 6
14.320037 ABIN2659757 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6
14.320037 ABIN2659882 FACS PerCP Mouse IgG1 kappa Log in to see HIT8a 3
14.320037 ABIN2660420 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see HIT8a 3
14.320037 ABIN2659564 FACS PE-Cy7 Mouse IgG1 kappa Log in to see RPA-T8 6

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(599), (495), (127), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(764), (458), (198), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(150), (143), (91), (55), (45), (36), (36), (33), (29), (27), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (14), (13), (11), (11), (11), (10), (9), (7), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1149), (445), (364), (256), (246), (236), (197), (185), (173), (35), (34), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN268292) Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN268292) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling ProductDetails: References for anti-CD8 antibody (ABIN268292) Images back to top
Application Notes Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details ProductDetails: References for anti-CD8 antibody (ABIN268292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-CD8 antibody (ABIN268292)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Karlsson, Johansen, Kahu, Macpherson, Brandtzaeg: "Hypersensitivity and oral tolerance in the absence of a secretory immune system." in: Allergy, Vol. 65, Issue 5, pp. 561-70, 2010

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009

Himoudi, Nabarro, Yan, Gilmour, Thrasher, Anderson: "Development of anti-PAX3 immune responses; a target for cancer immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 9, pp. 1381-95, 2007

Auray, Lacroix-Lamandé, Mancassola, Dimier-Poisson, Laurent: "Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection." in: Microbes and infection, Vol. 9, Issue 5, pp. 574-82, 2007

Sroga, Jones, Kigerl, McGaughy, Popovich: "Rats and mice exhibit distinct inflammatory reactions after spinal cord injury." in: The Journal of comparative neurology, Vol. 462, Issue 2, pp. 223-40, 2003

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling ProductDetails: References for anti-CD8 antibody (ABIN268292) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) anti-CD8a Molecule (CD8A) antibody
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Staining of Mouse...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Published ...
Immunofluorescence (IF) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) [ABIN268292] -...