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anti-Human CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1058
532
167
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Alexa Fluor 488
259
217
142
86
52
42
39
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
1617
452
356
342
284
281
255
237
214
36
33
25
22
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2657487
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086453 ABIN445798 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1150
0.0010086453 ABIN189246 ICC FACS IF IHC (p) IHC WB Rabbit IgG C-Term Log in to see EP1150Y
0.0010086453 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0010086453 ABIN2144215 ICC IHC (fro) FACS APC Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144259 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144260 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144261 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144354 ICC IHC (fro) FACS PE-Cy5 Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144217 ICC IHC (fro) FACS APC Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144258 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144352 ICC IHC (fro) FACS PE-Cy5 Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN1077936 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-198 Log in to see Polyclonal
0.0010086453 ABIN609897 ICC FACS ELISA WB Biotin Mouse IgG2a Log in to see UCHT4
0.0010086453 ABIN2145051 ICC IHC (fro) FACS Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2145050 ICC IHC (fro) FACS Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144769 ICC IHC (fro) FACS PE Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144767 ICC IHC (fro) FACS PE Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144475 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN2144477 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see 4ARIV11
0.0010086453 ABIN609898 ICC FACS ELISA WB FITC Mouse IgG2a Log in to see UCHT4

Similar anti-CD8 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 4 Antibodies
Co-Stimulation (CoSt) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
ELISA 181 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 824 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 163 Antibodies
Immunofluorescence (IF) 187 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 188 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1058), (532), (167), (68), (47), (40), (25), (22), (20), (20), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1285), (512), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Alexa Fluor 488
(259), (217), (142), (86), (52), (42), (39), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
(1617), (452), (356), (342), (284), (281), (255), (237), (214), (36), (33), (25), (22), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2657487) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone RPA-T8
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2657487) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2657487) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2657487) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD8 Antibody (ABIN2657487)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Rout, Else, Yue, Connole, Exley, Kaur: "Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection." in: PLoS ONE, Vol. 5, Issue 3, pp. e9787, 2010

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Thakral, Dobbins, Devine, Kavathas: "Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7431-42, 2008

Magidovich, Orr, Fass, Abdu, Yifrach: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007

Mack, Tucker, Sokol, Karrer, Kotzin, Whitington, Miller: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2657487) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Alexa Fluor 488) (ABIN2657487) anti-CD8a Molecule (CD8A) antibody (Alexa Fluor 488)