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anti-Human CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Epitope
AA 22-198
84
22
19
15
14
14
10
9
9
7
5
5
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
566
512
135
95
44
28
19
14
13
13
12
10
7
4
2
2
1
1
Host
Mouse
738
483
186
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
158
152
91
55
44
36
35
31
29
26
24
23
23
23
22
22
21
19
18
18
14
14
12
11
10
10
7
7
7
6
4
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC)
1150
431
339
254
243
231
186
183
173
27
24
22
20
15
13
12
11
9
8
6
6
6
6
5
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899645
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.964424 ABIN4896429 FACS ICC Alexa Fluor 488 Mouse IgG2b AA 22-198 Log in to see 37006
25.964424 ABIN4896428 FACS ICC Alexa Fluor 488 Mouse IgG2b AA 22-198 Log in to see 37006
1 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN440359 FACS ICC IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RFT-8
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN440358 FACS ICC IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see RFT-8
1 ABIN1077936 ICC IHC IP WB Rabbit IgG AA 22-198 Log in to see Polyclonal
1 ABIN4295991 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4296031 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296040 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296041 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296045 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296065 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296060 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296058 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296064 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296072 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296003 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa C-Term Log in to see C8-144B

Top referenced anti-CD8 antibody for Immunocytochemistry

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Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 22-198
(84), (22), (19), (15), (14), (14), (10), (9), (9), (7), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(566), (512), (135), (95), (44), (28), (19), (14), (13), (13), (12), (10), (7), (4), (2), (2), (1), (1)
Host Mouse
(738), (483), (186), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(158), (152), (91), (55), (44), (36), (35), (31), (29), (26), (24), (23), (23), (23), (22), (22), (21), (19), (18), (18), (14), (14), (12), (11), (10), (10), (7), (7), (7), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC)
(1150), (431), (339), (254), (243), (231), (186), (183), (173), (27), (24), (22), (20), (15), (13), (12), (11), (9), (8), (6), (6), (6), (6), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4899645) Images
Specificity Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human CD8 alpha , Ser22-Val198, Accession # NP_741969
Clone 37006
Isotype IgG2b

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN4899645) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Gene ID 925
NCBI Accession NP_741969
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN4899645) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN4899645) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD8 Antibody (ABIN4899645)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Chimote, Hajdu, Sfyris, Gleich, Wise-Draper, Casper, Conforti: "Kv1.3 Channels Mark Functionally Competent CD8+ Tumor-Infiltrating Lymphocytes in Head and Neck Cancer." in: Cancer research, Vol. 77, Issue 1, pp. 53-61, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4899645) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (AA 22-198) (ABIN4899645) anti-CD8a Molecule (CD8A) (AA 22-198) antibody