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anti-Human CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human, Mouse (Murine)
1070
537
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Rat
1305
518
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
263
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
1640
469
372
351
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN268292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009992593 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0009992593 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0009992593 ABIN2144260 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144258 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144354 ICC IHC (fro) FACS PE-Cy5 Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144215 ICC IHC (fro) FACS APC Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144217 ICC IHC (fro) FACS APC Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144259 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144261 ICC IHC (fro) FACS Biotin Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144352 ICC IHC (fro) FACS PE-Cy5 Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN1077936 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-198 Log in to see Polyclonal
0.0009992593 ABIN2144769 ICC IHC (fro) FACS PE Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2145050 ICC IHC (fro) FACS Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144477 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144475 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2144767 ICC IHC (fro) FACS PE Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN2145051 ICC IHC (fro) FACS Mouse IgG1 Log in to see 4ARIV11
0.0009992593 ABIN609898 ICC FACS ELISA WB FITC Mouse IgG2a Log in to see UCHT4
0.0009992593 ABIN609899 ICC FACS ELISA WB PE Mouse IgG2a Log in to see UCHT4
0.0009992593 ABIN440359 FACS ICC IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RFT-8

Top referenced anti-CD8 antibody for Immunocytochemistry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 464 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunofluorescence (IF) 89 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 87 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(1070), (537), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1305), (518), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(263), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1640), (469), (372), (351), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN268292) Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN268292) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN268292) Images back to top
Application Notes Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN268292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS. pH 7.4
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 Antibody (ABIN268292)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Karlsson, Johansen, Kahu, Macpherson, Brandtzaeg: "Hypersensitivity and oral tolerance in the absence of a secretory immune system." in: Allergy, Vol. 65, Issue 5, pp. 561-70, 2010

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009

Himoudi, Nabarro, Yan, Gilmour, Thrasher, Anderson: "Development of anti-PAX3 immune responses; a target for cancer immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 9, pp. 1381-95, 2007

Auray, Lacroix-Lamandé, Mancassola, Dimier-Poisson, Laurent: "Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection." in: Microbes and infection, Vol. 9, Issue 5, pp. 574-82, 2007

Sroga, Jones, Kigerl, McGaughy, Popovich: "Rats and mice exhibit distinct inflammatory reactions after spinal cord injury." in: The Journal of comparative neurology, Vol. 462, Issue 2, pp. 223-40, 2003

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN268292) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) anti-CD8a Molecule (CD8A) antibody
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Staining of Mouse...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Published ...
Immunofluorescence (IF) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) [ABIN268292] -...