anti-Human CD8 antibody for Immunofluorescence

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human, Mouse (Murine)
617
524
127
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
791
487
198
2
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
151
149
93
60
48
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
1205
445
364
257
248
236
199
185
181
39
35
20
16
13
11
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN268292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.920952 ABIN514147 IF IP ELISA Mouse IgG2b kappa AA 1-235, full length Log in to see 4B9
1 ABIN2659757 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2664858 IF WB Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN3024491 FACS IF IHC Mouse IgG1 Log in to see CLDA8a-1
1 ABIN3024488 FACS IF IHC Mouse IgG1 Log in to see C8-468
1 ABIN2659758 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN3024496 FACS IF IHC Mouse IgG1 C-Term, Cytoplasmic Domain Log in to see C8-468-C8-144B
1 ABIN5630736 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Rat IgG2a Log in to see YTS105-18
1 ABIN4295991 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN3024500 FACS IF IHC Mouse IgG1, kappa Log in to see SPM548
1 ABIN1837577 FACS IF IP Mouse IgG2a Log in to see MEM-31
1 ABIN4296003 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296053 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296064 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296070 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296005 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa C-Term Log in to see C8-144B
1 ABIN4296031 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG C-Term Log in to see C8-468-C8-144B
1 ABIN4296050 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa C-Term Log in to see C8-144B

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(617), (524), (127), (92), (44), (26), (22), (19), (13), (13), (13), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(791), (487), (198), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(151), (149), (93), (60), (48), (41), (40), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1205), (445), (364), (257), (248), (236), (199), (185), (181), (39), (35), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN268292) Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN268292) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN268292) Images back to top
Application Notes Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN268292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 antibody (ABIN268292)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Karlsson, Johansen, Kahu, Macpherson, Brandtzaeg: "Hypersensitivity and oral tolerance in the absence of a secretory immune system." in: Allergy, Vol. 65, Issue 5, pp. 561-70, 2010

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009

Himoudi, Nabarro, Yan, Gilmour, Thrasher, Anderson: "Development of anti-PAX3 immune responses; a target for cancer immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 9, pp. 1381-95, 2007

Auray, Lacroix-Lamandé, Mancassola, Dimier-Poisson, Laurent: "Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection." in: Microbes and infection, Vol. 9, Issue 5, pp. 574-82, 2007

Sroga, Jones, Kigerl, McGaughy, Popovich: "Rats and mice exhibit distinct inflammatory reactions after spinal cord injury." in: The Journal of comparative neurology, Vol. 462, Issue 2, pp. 223-40, 2003

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN268292) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) anti-CD8a Molecule (CD8A) antibody
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Staining of Mouse...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Published ...
Immunofluorescence (IF) image for anti-CD8a Molecule (CD8A) antibody (ABIN268292) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) [ABIN268292] -...