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anti-Human CD8 antibody for Immunofluorescence

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Human, Mouse (Murine)
1050
535
161
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rat
1284
509
215
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
261
219
141
81
57
45
42
38
34
30
29
28
26
26
25
25
25
24
21
21
19
17
16
15
11
10
10
10
9
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
1626
451
448
316
270
267
261
245
234
39
33
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN268292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.4274025 ABIN514147 IF IP ELISA Mouse IgG2b kappa AA 1-235, full length Log in to see 4B9
1 ABIN1112229 FACS IF APC Mouse IgG2a Log in to see MEM-31 3
1 ABIN1112232 FACS IF PerCP Mouse IgG1 Log in to see 143-44 3
1 ABIN1112231 FACS IF CF405M Mouse IgG1 Log in to see 143-44 3
1 ABIN1112228 FACS IF APC Mouse IgG1 Log in to see 143-44 3
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659552 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see SK1 7
1 ABIN2659757 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2664827 FACS IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see SK1 7
1 ABIN2664858 IF WB Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2659553 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see SK1 7
1 ABIN2659758 FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN2851719 FACS IF PE Mouse IgG1 Log in to see
1 ABIN2851741 FACS IF PE-Cy5 Mouse IgG1 Log in to see
1 ABIN2851754 FACS IF Biotin Mouse IgG1 Log in to see
1 ABIN2851766 FACS IF APC Mouse IgG1 Log in to see
1 ABIN2851649 FACS IF FITC Mouse IgG1 Log in to see
1 ABIN1112230 FACS IF Biotin Mouse IgG1 Log in to see 143-44 3
1 ABIN1106563 Func IF MCA Mouse IgG2a Log in to see WT82

Top referenced anti-CD8 antibody for Immunofluorescence

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
Cytotoxicity Test (CyTox) 10 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 467 Antibodies
Functional Studies (Func) 26 Antibodies
Immunocytochemistry (ICC) 79 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 126 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 125 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human, Mouse (Murine)
(1050), (535), (161), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1284), (509), (215), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(261), (219), (141), (81), (57), (45), (42), (38), (34), (30), (29), (28), (26), (26), (25), (25), (25), (24), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC)
(1626), (451), (448), (316), (270), (267), (261), (245), (234), (39), (33), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN268292) Images
Specificity This antibody recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
Purification Protein G purified
Immunogen Mouse spleen cells
Clone YTS105-18
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN268292) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 26 kDa
Gene ID 925
UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN268292) Images back to top
Application Notes Flow Cytometry 1:50-1:100, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. IHC-P: It has been reported that the antibody did not work in paraffin embedded mouse spleen tissue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN268292) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 Antibody (ABIN268292)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Karlsson, Johansen, Kahu, Macpherson, Brandtzaeg: "Hypersensitivity and oral tolerance in the absence of a secretory immune system." in: Allergy, Vol. 65, Issue 5, pp. 561-70, 2010

Lacroix-Lamandé, Rochereau, Mancassola, Barrier, Clauzon, Laurent: "Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation." in: PLoS ONE, Vol. 4, Issue 12, pp. e8291, 2009

Himoudi, Nabarro, Yan, Gilmour, Thrasher, Anderson: "Development of anti-PAX3 immune responses; a target for cancer immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 9, pp. 1381-95, 2007

Auray, Lacroix-Lamandé, Mancassola, Dimier-Poisson, Laurent: "Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection." in: Microbes and infection, Vol. 9, Issue 5, pp. 574-82, 2007

Sroga, Jones, Kigerl, McGaughy, Popovich: "Rats and mice exhibit distinct inflammatory reactions after spinal cord injury." in: The Journal of comparative neurology, Vol. 462, Issue 2, pp. 223-40, 2003

Wise, Bemelman, Cobbold, Waldmann: "Linked suppression of skin graft rejection can operate through indirect recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5813-6, 1998

Qin, Wise, Cobbold, Leong, Kong, Parnes, Waldmann: "Induction of tolerance in peripheral T cells with monoclonal antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2737-45, 1991

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN268292) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) anti-CD8a Molecule (CD8A) antibody
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunohistochemistry: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Staining of Mouse...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunohistochemistry-Frozen: CD8 alpha Antibody (YTS105.18) [ABIN268292] - Published ...
Immunofluorescence (IF) image for anti-CD8 antibody (CD8a Molecule) (ABIN268292) Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (YTS105.18) [ABIN268292] -...