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anti-Mouse (Murine) CD8 antibody for Blocking Reagent

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1069
537
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Rat
1303
518
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to APC
264
219
144
85
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
1638
470
373
350
290
283
264
245
215
37
33
25
23
17
13
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2658875
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2660735 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10
0.0008709465 ABIN2657489 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
0.0008709465 ABIN1177008 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53-6-7 25
0.0008709465 ABIN2658876 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 11
0.0008709465 ABIN2660736 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 12
0.0008709465 ABIN2657490 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
0.0008709465 ABIN2689432 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53 24
0.0008709465 ABIN2689433 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53

Top referenced anti-CD8 antibody for Blocking Reagent

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1069), (537), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(1303), (518), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to APC
(264), (219), (144), (85), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(1638), (470), (373), (350), (290), (283), (264), (245), (215), (37), (33), (25), (23), (17), (13), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2658875) Images
Purification The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2658875) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2658875) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2658875) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD8 Antibody (ABIN2658875)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Bouwer, Alberti-Segui, Montfort, Berkowitz, Higgins: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006

Kamimura, Sawa, Sato, Agung, Hirano, Murakami: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006

Hata, Sakaguchi, Yoshitomi, Iwakura, Sekikawa, Azuma, Kanai, Moriizumi, Nomura, Nakamura, Sakaguchi: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004

Fan, Kouda, Nakamura, Takeuchi: "Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 226, Issue 11, pp. 1045-50, 2001

Ellmeier, Sawada, Littman: "The regulation of CD4 and CD8 coreceptor gene expression during T cell development." in: Annual review of immunology, Vol. 17, pp. 523-54, 1999

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Seaman, Tsu, Herzenberg: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, 1981

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2658875) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (APC) (ABIN2658875) anti-CD8a Molecule (CD8A) antibody (APC)