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anti-Mouse (Murine) CD8 antibody for Cytotoxicity Test

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
766
403
130
68
28
27
25
20
20
16
15
12
12
11
11
10
10
9
8
8
3
3
2
2
2
2
1
1
1
Host
Mouse
960
391
161
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
243
190
122
86
30
30
27
18
18
17
16
15
13
11
10
10
10
9
8
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox), Flow Cytometry (FACS)
1177
282
232
188
136
111
105
81
69
36
33
25
23
16
14
10
7
6
6
6
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN114178
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087398855 ABIN114179 CyTox Mouse IgM Log in to see AD4(15) 2
0.00087398855 ABIN951386 CyTox FACS Rat IgG2b Log in to see YTS169-4 4
0.00087398855 ABIN951399 CyTox FACS Mouse IgM Log in to see AD4(15) 1
0.00087398855 ABIN951396 CyTox Mouse IgG3 Log in to see 49-31-1
0.00087398855 ABIN951395 CyTox FACS Mouse IgG3 Log in to see 49-31-1
0.00087398855 ABIN133860 CyTox Mouse IgG3 Log in to see
0.00087398855 ABIN951400 CyTox Mouse IgM Log in to see AD4(15)
0.00087398855 ABIN1967953 CyTox FACS Mouse IgM Log in to see NYRmCD8

Top referenced anti-CD8 antibody for Cytotoxicity Test

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 345 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 72 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Intracellular Flow Cytometry (ICFC) 7 Antibodies
Separation (Sep) 2 Antibodies
Western Blotting (WB) 20 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(766), (403), (130), (68), (28), (27), (25), (20), (20), (16), (15), (12), (12), (11), (11), (10), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(960), (391), (161)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(243), (190), (122), (86), (30), (30), (27), (18), (18), (17), (16), (15), (13), (11), (10), (10), (10), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox), Flow Cytometry (FACS)
(1177), (282), (232), (188), (136), (111), (105), (81), (69), (36), (33), (25), (23), (16), (14), (10), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114178) Images
Specificity This Ly-2.1 Monoclonal Antibody reacts with a sub-population of lymphocytes from mouse strains expressing the Ly 2.1 (CD8a) phenotype but does not react with lymphocytes from mouse strains expressing the Ly 2.2 phenotype.
Purification Ascites
Immunogen Recipient: 129/ReJ. Donor: CBA. Fusion Partner: Spleen from immunized recipient fused with Myeloma P3 NSI-Ag 4-1.
Clone 49-31-1
Isotype IgG3

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114178) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114178) Images back to top
Application Notes Flow Cytometry Analysis. Cytotoxicity Analysis (see Protocols). Functional Testing (see Protocols). Cytotoxicity Depletion Assays (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol CYTOTOXICITY ANALYSIS: Method for Determining percent of Ly 2. 1+ cells in a population: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-M density cell separation medium. After washing, adjust thecell concentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Low-Tox®-M Rabbit Complement diluted to therecommended concentration in Cytotoxicity Medium. 6. Incubate for 60 minutes at 37°C. 7. Place on ice. 8. Add Trypan Blue, 10% by volume of 1% Trypan Blue (w/v) added 3-5 minutes beforescoring works well. Score live versus dead cells in a hemacytometer. Results - Antibody Titration: Cell Source: ThymusDonor: B6-Ly-2a3a (Ly 2. 1)Cell
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114178) Images back to top
Format Liquid
Reconstitution Restore with 0.5 mL of distilled water.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-CD8 Antibody (ABIN114178)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Ahn, Maruo, Tomura, Mu, Hamaoka, Nakanishi, Clark, Kurimoto, Okamura, Fujiwara: "A mechanism underlying synergy between IL-12 and IFN-gamma-inducing factor in enhanced production of IFN-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2125-31, 1997

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114178) back to top
Supplier Images
 image for anti-CD8 antibody (CD8a Molecule) (ABIN114178) Cytotoxic Index (C. I.) can be calculated as follows: