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anti-Mouse (Murine) CD8 antibody for Cytotoxicity Test

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1070
537
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1303
519
205
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
263
219
144
87
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox)
1640
470
373
351
290
285
264
246
215
37
33
25
23
16
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN114179
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087021245 ABIN114178 CyTox FACS Mouse IgG3 Log in to see 49-31-1 1
0.00087021245 ABIN951386 CyTox FACS Rat IgG2b Log in to see YTS169-4 4
0.00087021245 ABIN951399 CyTox FACS Mouse IgM Log in to see AD4(15) 1
0.00087021245 ABIN951396 CyTox Mouse IgG3 Log in to see 49-31-1
0.00087021245 ABIN951395 CyTox FACS Mouse IgG3 Log in to see 49-31-1
0.00087021245 ABIN133860 CyTox Mouse IgG3 Log in to see
0.00087021245 ABIN951400 CyTox Mouse IgM Log in to see AD4(15)
0.00087021245 ABIN1967953 CyTox FACS Mouse IgM Log in to see NYRmCD8

Top referenced anti-CD8 antibody for Cytotoxicity Test

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 464 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunofluorescence (IF) 89 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 87 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1070), (537), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1303), (519), (205)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(263), (219), (144), (87), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox)
(1640), (470), (373), (351), (290), (285), (264), (246), (215), (37), (33), (25), (23), (16), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114179) Images
Specificity This anti-mouse CD8a (Ly 2.2) monoclonal antibody reacts with a subpopulation of T-lymphocytes from mouse strains expressing the Ly-2.2 phenotype but does not react with lymphocytes from strains expressing the Ly-2.1 phenotype.
Purification Ascites
Immunogen C57BL/6, Donor: B6-Ly-2a spleen, Fusion Partner: Myeloma P3/X63-Ag
Clone AD4(15)
Isotype IgM

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114179) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114179) Images back to top
Application Notes This clone has been shown to work in both cytotoxicity assays and flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Low-Tox®-M Rabbit Complementc diluted to therecommended concentration in Cytotoxicity Medium. 6. Incubate for 60 minutes at 37°C. 7. Place on ice. 8. Add Trypan Blue, 10% by volume of 1% Trypan Blue (w/v) added 3-5 minutes beforescoring works well. Score live versus dead cells in a hemacytometer. Results - Antibody Titration: Cell Source: ThymusDonor: C57BL/6 (Ly-2. 2)Cell
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114179) Images back to top
Format Liquid
Reconstitution Restore with 0.5 mL of cold distilled water.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-CD8 Antibody (ABIN114179)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Keim, Gottlieb, Nelson, Vogels,: "2003 JCO Orthodontic Practice Study. Part 1. Trends." in: Journal of clinical orthodontics : JCO, Vol. 37, Issue 10, pp. 545-53, 2003

Raulet, Gottlieb, Bevan: "Fractionation of lymphocyte populations with monoclonal antibodies specific for LYT-2.2 and LYT-3.1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 3, pp. 1136-43, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114179) back to top
Supplier Images
 image for anti-CD8 antibody (CD8a Molecule) (ABIN114179) Cytotoxic Index