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anti-Mouse (Murine) CD8 antibody for Cytotoxicity Test

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine)
1051
535
161
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Mouse
1283
510
215
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
261
219
141
81
57
45
42
38
34
30
29
28
26
26
25
25
25
24
21
21
19
17
16
15
11
10
10
10
9
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox)
1627
452
449
316
271
267
261
246
234
39
33
25
24
23
17
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN114179
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN114178 CyTox FACS Mouse IgG3 Log in to see 49-31-1 1
1 ABIN951386 CyTox FACS Rat IgG2b Log in to see YTS169-4 4
1 ABIN951399 CyTox FACS Mouse IgM Log in to see AD4(15) 1
1 ABIN951396 CyTox Mouse IgG3 Log in to see 49-31-1
1 ABIN951395 CyTox FACS Mouse IgG3 Log in to see 49-31-1
1 ABIN133860 CyTox Mouse IgG3 Log in to see
1 ABIN951400 CyTox Mouse IgM Log in to see AD4(15)
1 ABIN1967953 CyTox FACS Mouse IgM Log in to see NYRmCD8
1 ABIN133865 CyTox Mouse IgM Log in to see

Top referenced anti-CD8 antibody for Cytotoxicity Test

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
Cytotoxicity Test (CyTox) 10 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 467 Antibodies
Functional Studies (Func) 26 Antibodies
Immunocytochemistry (ICC) 79 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 126 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 125 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1051), (535), (161), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1283), (510), (215), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(261), (219), (141), (81), (57), (45), (42), (38), (34), (30), (29), (28), (26), (26), (25), (25), (25), (24), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox)
(1627), (452), (449), (316), (271), (267), (261), (246), (234), (39), (33), (25), (24), (23), (17), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114179) Images
Specificity This anti-mouse CD8a (Ly 2.2) monoclonal antibody reacts with a subpopulation of T-lymphocytes from mouse strains expressing the Ly-2.2 phenotype but does not react with lymphocytes from strains expressing the Ly-2.1 phenotype.
Purification Ascites
Immunogen C57BL/6, Donor: B6-Ly-2a spleen, Fusion Partner: Myeloma P3/X63-Ag
Clone AD4(15)
Isotype IgM

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114179) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114179) Images back to top
Application Notes This clone has been shown to work in both cytotoxicity assays and flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Low-Tox®-M Rabbit Complementc diluted to therecommended concentration in Cytotoxicity Medium. 6. Incubate for 60 minutes at 37°C. 7. Place on ice. 8. Add Trypan Blue, 10% by volume of 1% Trypan Blue (w/v) added 3-5 minutes beforescoring works well. Score live versus dead cells in a hemacytometer. Results - Antibody Titration: Cell Source: ThymusDonor: C57BL/6 (Ly-2. 2)Cell
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114179) Images back to top
Format Liquid
Reconstitution Restore with 0.5 mL of cold distilled water.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-CD8 Antibody (ABIN114179)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Keim, Gottlieb, Nelson, Vogels,: "2003 JCO Orthodontic Practice Study. Part 1. Trends." in: Journal of clinical orthodontics : JCO, Vol. 37, Issue 10, pp. 545-53, 2003

Raulet, Gottlieb, Bevan: "Fractionation of lymphocyte populations with monoclonal antibodies specific for LYT-2.2 and LYT-3.1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 3, pp. 1136-43, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114179) back to top
Supplier Images
 image for anti-CD8 antibody (CD8a Molecule) (ABIN114179) Cytotoxic Index