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anti-Mouse (Murine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1069
537
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Rat
1303
518
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
263
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1638
470
374
351
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN114174
$ 775.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN114170 FACS Biotin Rat IgG2a Log in to see CT-CD8a 1
0.30226976 ABIN114164 FACS Biotin Rat IgG2b Log in to see YTS169-4 1
0.30226976 ABIN114166 FACS IHC (fro) FITC Rat IgG2b Log in to see YTS169-4 4
0.30226976 ABIN114171 FACS Biotin Rat IgG2a Log in to see CT-CD8a 1
0.30226976 ABIN114168 FACS PE Rat IgG2b Log in to see YTS169-4 4
0.30226976 ABIN118129 FACS FITC Rat IgG2a Log in to see KT15 2
0.30226976 ABIN118134 FACS PE Rat IgG2a Log in to see KT15 1
0.30226976 ABIN118177 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
0.30226976 ABIN118133 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 14
0.30226976 ABIN114167 FACS IHC (fro) Rat IgG2b Log in to see YTS169-4 6
0.30226976 ABIN114176 FACS PE Rat IgG2a Log in to see CT-CD8a 2
0.30226976 ABIN114177 FACS PE Rat IgG2a Log in to see CT-CD8a 2
0.30226976 ABIN114169 FACS APC Rat IgG2a Log in to see CT-CD8a 2
0.30226976 ABIN356190 FACS IHC (fro) IP FITC Rat IgG2a Log in to see 07-06-53 7
0.30226976 ABIN2658875 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 10
0.30226976 ABIN2659625 FACS PE-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10
0.30226976 ABIN2659759 FACS PE/Dazzle™ 594 Rat IgG2a kappa Log in to see 53-6-7 10
0.30226976 ABIN2660735 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10
0.30226976 ABIN2657489 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
0.30226976 ABIN2657864 FACS Alexa Fluor 647 Rat IgG2a kappa Log in to see 53-6-7 10

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1069), (537), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(1303), (518), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(263), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1638), (470), (374), (351), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114174) Images
Specificity This anti-mouse CD8a antigen monoclonal antibody recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/β heterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells1. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive cells. For this reason antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive cells.
Purification Protein G Chromatography
Immunogen Murine thymocytes
Clone CT-CD8a
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114174) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114174) Images back to top
Application Notes Flow Cytometry Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 1x10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114174) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN114174)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Weber, Shi, Heise, Warner, Mahvi: "Interleukin-12 gene transfer results in CD8-dependent regression of murine CT26 liver tumors." in: Annals of surgical oncology, Vol. 6, Issue 2, pp. 186-94, 1999

Tomonari, Spencer: "Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity." in: International immunology, Vol. 2, Issue 12, pp. 1189-94, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114174) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN114174) Representative Histogram - Cell Source: CD3e Positive Spleen Cells Percentage of cell...