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anti-Mouse (Murine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine)
1051
535
161
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rat
1284
509
215
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Biotin
261
219
140
81
57
45
42
38
34
30
29
28
26
26
25
25
25
24
21
21
19
17
16
15
11
10
10
10
9
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1626
452
449
316
271
267
261
246
234
39
33
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN114170
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.739399 ABIN114166 FACS IHC (fro) FITC Rat IgG2b Log in to see YTS169-4 4
13.739399 ABIN114164 FACS Biotin Rat IgG2b Log in to see YTS169-4 1
13.739399 ABIN114174 FACS FITC Rat IgG2a Log in to see CT-CD8a 2
13.739399 ABIN114171 FACS Biotin Rat IgG2a Log in to see CT-CD8a 1
13.739399 ABIN114168 FACS PE Rat IgG2b Log in to see YTS169-4 4
13.739399 ABIN118129 FACS FITC Rat IgG2a Log in to see KT15 2
13.739399 ABIN118177 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
13.739399 ABIN118133 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 14
13.739399 ABIN118134 FACS PE Rat IgG2a Log in to see KT15 1
13.739399 ABIN114167 FACS IHC (fro) Rat IgG2b Log in to see YTS169-4 6
13.739399 ABIN114177 FACS PE Rat IgG2a Log in to see CT-CD8a 2
13.739399 ABIN114176 FACS PE Rat IgG2a Log in to see CT-CD8a 2
13.739399 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
13.739399 ABIN114169 FACS APC Rat IgG2a Log in to see CT-CD8a 2
13.739399 ABIN356190 FACS IHC (fro) IP FITC Rat IgG2a Log in to see 07-06-53 7
13.739399 ABIN4295972 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7 7
13.739399 ABIN4898429 FACS Fluorescein Rat IgG2a Log in to see 53-6-7 2
13.739399 ABIN4898426 FACS PerCP Rat IgG2a Log in to see 53-6-7 1
13.739399 ABIN2663978 FACS PE Rat IgG2a kappa Log in to see 53-6-7 10
13.739399 ABIN2660735 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
Cytotoxicity Test (CyTox) 10 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 467 Antibodies
Functional Studies (Func) 26 Antibodies
Immunocytochemistry (ICC) 79 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 126 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 125 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1051), (535), (161), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1284), (509), (215), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Biotin
(261), (219), (140), (81), (57), (45), (42), (38), (34), (30), (29), (28), (26), (26), (25), (25), (25), (24), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1626), (452), (449), (316), (271), (267), (261), (246), (234), (39), (33), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114170) Images
Specificity This anti-mouse CD8a antigen monoclonal antibody recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/β heterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells1. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive T cells. For this reason antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive cells.
Purification Protein G Chromatography
Immunogen Murine thymocytes
Clone CT-CD8a
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114170) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114170) Images back to top
Application Notes Flow Cytometry Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 125 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-PE) at a 1: 5 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Representative Dot PlotMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 125 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2a
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114170) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.1 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN114170)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Tomonari, Spencer: "Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity." in: International immunology, Vol. 2, Issue 12, pp. 1189-94, 1991

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114170) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Biotin) (ABIN114170) Representative Dot Plot