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anti-Mouse (Murine) CD8 antibody for Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine)
1085
550
167
77
50
41
25
25
24
23
22
20
19
17
13
13
13
12
12
12
12
11
10
3
3
2
2
2
1
1
1
1
Host
Rat
1319
525
219
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Biotin
271
224
142
82
59
47
44
40
35
32
30
28
28
27
27
27
26
26
22
22
19
19
17
15
11
10
10
10
9
7
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1671
479
455
323
272
266
258
229
225
39
32
25
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN114164
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.470028 ABIN114170 FACS Biotin Rat IgG2a Log in to see CT-CD8a 1
13.470028 ABIN114166 FACS IHC (fro) FITC Rat IgG2b Log in to see YTS169-4 4
13.470028 ABIN114174 FACS FITC Rat IgG2a Log in to see CT-CD8a 2
13.470028 ABIN114171 FACS Biotin Rat IgG2a Log in to see CT-CD8a 1
13.470028 ABIN114168 FACS PE Rat IgG2b Log in to see YTS169-4 4
13.470028 ABIN118129 FACS FITC Rat IgG2a Log in to see KT15 2
13.470028 ABIN118177 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
13.470028 ABIN118134 FACS PE Rat IgG2a Log in to see KT15 1
13.470028 ABIN118133 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 14
13.470028 ABIN114167 FACS IHC (fro) Rat IgG2b Log in to see YTS169-4 6
13.470028 ABIN114177 FACS PE Rat IgG2a Log in to see CT-CD8a 2
13.470028 ABIN114176 FACS PE Rat IgG2a Log in to see CT-CD8a 2
13.470028 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
13.470028 ABIN114169 FACS APC Rat IgG2a Log in to see CT-CD8a 2
13.470028 ABIN356190 FACS IHC (fro) IP FITC Rat IgG2a Log in to see 07-06-53 7
13.470028 ABIN671391 FACS IF (p) IHC (p) WB Rabbit IgG AA 50-90 Log in to see Polyclonal 8
13.470028 ABIN4295972 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7 7
13.470028 ABIN4898428 FACS Fluorescein Rat IgG2a Log in to see 53-6-7 2
13.470028 ABIN4898426 FACS PerCP Rat IgG2a Log in to see 53-6-7 1
13.470028 ABIN4898429 FACS Fluorescein Rat IgG2a Log in to see 53-6-7 2

Top referenced anti-CD8 antibody for Flow Cytometry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
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Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytometry by Time of Flight (CyTOF) 7 Antibodies
Cytotoxicity Test (CyTox) 10 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 9 Antibodies
Flow Cytometry (FACS) 484 Antibodies
Functional Studies (Func) 20 Antibodies
Immunocytochemistry (ICC) 67 Antibodies
Immunofluorescence (IF) 73 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 138 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 125 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 69 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1085), (550), (167), (77), (50), (41), (25), (25), (24), (23), (22), (20), (19), (17), (13), (13), (13), (12), (12), (12), (12), (11), (10), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1319), (525), (219), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Biotin
(271), (224), (142), (82), (59), (47), (44), (40), (35), (32), (30), (28), (28), (27), (27), (27), (26), (26), (22), (22), (19), (19), (17), (15), (11), (10), (10), (10), (9), (7), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1671), (479), (455), (323), (272), (266), (258), (229), (225), (39), (32), (25), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114164) Images
Specificity This anti-mouse CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/ suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology.
Purification Protein G Chromatography
Immunogen Murine thymocytes Donor: (LOU X DA) F1 rats Fusion Partner: Myeloma Y3/Ag 1.2.3
Clone YTS169-4
Isotype IgG2b

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114164) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114164) Images back to top
Application Notes Flow Cytometry Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. The stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue DistributionMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2bResults - Strain DistributionCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 0. 2 µg/10e6 cellsStrains Tested: BALB/c, CBA/J, C3H/He,C57BL/6Positive: BALB/c, CBA/J, C3H/He,C57BL/6Negative: none
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114164) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN114164)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Aqel, Clark, Cobbold, Waldmann: "Immunohistological screening in the selection of monoclonal antibodies: the use of isotype-specific antiglobulins." in: Journal of immunological methods, Vol. 69, Issue 2, pp. 207-14, 1984

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114164) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (Biotin) (ABIN114164) FLOW CYTOMETRY ANALYSIS Cell Source: Thymus Percentage of cells stained above control...