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anti-Mouse (Murine) CD8 antibody for Functional Studies

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1026
534
164
73
47
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rat
1274
509
193
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
257
217
138
81
56
44
41
37
33
29
28
28
26
25
24
24
24
24
20
20
19
17
16
14
12
11
10
10
10
6
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func)
1616
439
351
337
269
262
240
235
199
38
37
25
23
17
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN356191
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008756174 ABIN356192 Func FACS IHC (fro) IP Rat IgG2a Log in to see 07-06-53 7
0.0008756174 ABIN1177008 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53-6-7 25
0.0008756174 ABIN2749035 FACS Func ICC IHC (f) IHC (p) IP Rat IgG2a Log in to see 53-6-7 8
0.0008756174 ABIN349708 FACS Func ICC IHC (f) IHC (p) IP FITC Rat IgG2a Log in to see 53-6-7 9
0.0008756174 ABIN349711 FACS Func ICC IHC (f) IHC (p) IP PE Rat IgG2a Log in to see 53-6-7 8
0.0008756174 ABIN457303 FACS Func ICC IHC (f) IHC (p) IP APC Rat IgG2a Log in to see 53-6-7 7
0.0008756174 ABIN2749187 Func IHC (f) ICC FACS IP IHC (p) Rat IgG2a Log in to see 53-6-7 8
0.0008756174 ABIN3200064 FACS Func ICC IHC IP PerCP Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN3200061 FACS Func ICC IHC IP APC Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN3200062 FACS Func ICC IHC IP FITC Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN3200063 FACS Func ICC IHC IP PE Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN2567970 Func FACS IHC Rat IgG2b Log in to see 2-43
0.0008756174 ABIN2567999 Func FACS IP IHC Rat IgG2a kappa Log in to see 53-6-7
0.0008756174 ABIN2568001 Func FACS IP IHC Rat IgG2a kappa Log in to see 53-6-7
0.0008756174 ABIN2567998 Func FACS IP IHC Rat IgG2a kappa Log in to see 53-6-7
0.0008756174 ABIN2568000 Func FACS IP IHC Rat IgG2a kappa Log in to see 53-6-7
0.0008756174 ABIN3200059 FACS Func ICC IHC IP Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN2689433 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53
0.0008756174 ABIN3200060 FACS Func ICC IHC IP Rat IgG2a Log in to see 53-6-7
0.0008756174 ABIN3037651 Func ICC FACS IF IP IHC PerCP Rat IgG2a Log in to see

Top referenced anti-CD8 antibody for Functional Studies

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 474 Antibodies
Functional Studies (Func) 26 Antibodies
Immunocytochemistry (ICC) 81 Antibodies
Immunofluorescence (IF) 87 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 86 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 131 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Inhibition Assay (InhA) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1026), (534), (164), (73), (47), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1274), (509), (193), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(257), (217), (138), (81), (56), (44), (41), (37), (33), (29), (28), (28), (26), (25), (24), (24), (24), (24), (20), (20), (19), (17), (16), (14), (12), (11), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func)
(1616), (439), (351), (337), (269), (262), (240), (235), (199), (38), (37), (25), (23), (17), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN356191) Images
Specificity This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Characteristics Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Endotoxin Level Low Endotoxin
Clone 07-06-53
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN356191) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN356191) Images back to top
Application Notes Functional Assay: In vivo and in vitro depletion of CD8a+ cells. (Ref.1,4,5)Fractionation of CD8a+ T cells. (Ref.10)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN356191) Images back to top
Concentration 0.5 mg/mL
Buffer PBS without preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD8 Antibody (ABIN356191)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991

Swain, Dialynas, Fitch, English: "Monoclonal antibody to L3T4 blocks the function of T cells specific for class 2 major histocompatibility complex antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 3, pp. 1118-23, 1984

Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980