anti-Mouse (Murine) CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine), Rat (Rattus)
618
524
126
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Rat
791
487
198
2
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
151
149
93
60
48
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
1205
445
364
256
247
236
199
185
181
38
35
20
16
13
11
11
9
7
6
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN4295972
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.022951 ABIN4900730 CyTOF FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
27.022951 ABIN4900731 CyTOF FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
27.022951 ABIN4900660 FACS ICC Biotin Rat IgG2a Log in to see 53-6-7 2
1 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
1 ABIN1862305 ICC IHC IP WB Rabbit IgG AA 27-247 Log in to see Polyclonal
1 ABIN4898431 FACS ICC Alexa Fluor 488 Rat IgG2a Log in to see 53-6-7
1 ABIN4898430 FACS ICC Alexa Fluor 488 Rat IgG2a Log in to see 53-6-7
1 ABIN2749035 FACS Func ICC IHC (f) IHC (p) IP Rat IgG2a Log in to see 53-6-7 8
1 ABIN349708 FACS Func ICC IHC (f) IHC (p) IP FITC Rat IgG2a Log in to see 53-6-7 9
1 ABIN349711 FACS Func ICC IHC (f) IHC (p) IP PE Rat IgG2a Log in to see 53-6-7 8
1 ABIN457303 FACS Func ICC IHC (f) IHC (p) IP APC Rat IgG2a Log in to see 53-6-7 7
1 ABIN5630736 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Rat IgG2a Log in to see YTS105-18
1 ABIN4295971 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296120 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 700 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296127 FACS ICC IF IHC (fro) IHC (p) DyLight 405 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296121 FACS ICC IF IHC (fro) IHC (p) DyLight 650 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296117 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296119 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 647 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296122 FACS ICC IF IHC (fro) IHC (p) DyLight 680 Rat IgG2a kappa Log in to see 53-6-7
1 ABIN4296123 FACS ICC IF IHC (fro) IHC (p) DyLight 488 Rat IgG2a kappa Log in to see 53-6-7

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(618), (524), (126), (92), (44), (26), (22), (19), (13), (13), (13), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Rat
(791), (487), (198), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(151), (149), (93), (60), (48), (41), (40), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
(1205), (445), (364), (256), (247), (236), (199), (185), (181), (38), (35), (20), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4295972) Images
Purification Protein A or G purified
Immunogen Mouse thymus or spleen.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN4295972) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 27 kDa
Gene ID 925
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN4295972) Images back to top
Application Notes Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Cell depletion, Inhibition of T Cell Function, CyTOF-readyEach lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 μg/10^6 cells in 100 μL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN4295972) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 antibody (ABIN4295972)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Cohen, Shani, Raz, Sharon, Hoffman, Abramovitz, Erez: "Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1." in: Oncogene, Vol. 36, Issue 31, pp. 4457-4468, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Li, Wu, Tang, Li, Feng, Yao: "In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice." in: Cancer biotherapy & radiopharmaceuticals, Vol. 31, Issue 9, pp. 317-323, 2016 (Sample species: Human).

Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: "Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice." in: Neuropharmacology, Vol. 81, pp. 237-43, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kheradmand, Wang, Gibly, Zhang, Holland, Tasch, Graham, Kaufman, Miller, Shea, Luo: "Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions." in: Biomaterials, Vol. 32, Issue 20, pp. 4517-24, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Bankoti, Burnett, Navarro, Miller, Rase, Shepherd: "Effects of TCDD on the fate of naive dendritic cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 2, pp. 422-34, 2010

McLellan, Kapp, Eggert, Linden, Bommhardt, Bröcker, Kämmerer, Kämpgen: "Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression." in: Blood, Vol. 99, Issue 6, pp. 2084-93, 2002

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4295972) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine s...
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha e...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of C...
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by...
Immunocytochemistry (ICC) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion...
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blo...
Immunocytochemistry (ICC) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocyt...
Immunocytochemistry (ICC) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marr...
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) antibody (ABIN4295972) Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen s...