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anti-Mouse (Murine) CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Mouse (Murine), Rat (Rattus)
1057
550
164
77
50
41
22
22
20
20
19
16
15
13
13
12
12
12
11
11
10
10
8
3
3
2
2
2
2
1
1
1
1
Host
Rat
1288
522
221
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
264
220
141
81
58
46
43
39
35
31
29
28
27
26
26
26
26
25
21
21
19
17
16
15
11
10
10
10
9
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
1651
465
446
318
270
268
267
245
231
39
38
25
24
23
18
14
11
10
7
7
6
6
6
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4295972
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.229177 ABIN4900660 FACS ICC Biotin Rat IgG2a Log in to see 53-6-7 2
26.229177 ABIN4900731 FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
26.229177 ABIN4900730 FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
1 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
1 ABIN4295939 FACS ICC IF Rat IgG2b kappa Log in to see 2-43
1 ABIN349708 FACS Func ICC IHC (f) IHC (p) IP FITC Rat IgG2a Log in to see 53-6-7 9
1 ABIN349711 FACS Func ICC IHC (f) IHC (p) IP PE Rat IgG2a Log in to see 53-6-7 8
1 ABIN2749035 FACS Func ICC IHC (f) IHC (p) IP Rat IgG2a Log in to see 53-6-7 8
1 ABIN457303 FACS Func ICC IHC (f) IHC (p) IP APC Rat IgG2a Log in to see 53-6-7 7
1 ABIN1862305 ICC IHC IP WB Rabbit IgG AA 27-247 Log in to see Polyclonal
1 ABIN4898430 FACS ICC Alexa Fluor 488 Rat IgG2a Log in to see 53-6-7
1 ABIN4898431 FACS ICC Alexa Fluor 488 Rat IgG2a Log in to see 53-6-7
1 ABIN3200064 FACS Func ICC IHC IP PerCP Rat IgG2a Log in to see 53-6-7
1 ABIN4295961 FACS ICC IF PerCP Rat IgG2b kappa Log in to see 2-43
1 ABIN4295963 FACS ICC IF DyLight 550 Rat IgG2b kappa Log in to see 2-43
1 ABIN4295964 FACS ICC IF DyLight 350 Rat IgG2b kappa Log in to see 2-43
1 ABIN4295965 FACS ICC IF DyLight 405 Rat IgG2b kappa Log in to see 2-43
1 ABIN4295960 FACS ICC IF DyLight 755 Rat IgG2b kappa Log in to see 2-43
1 ABIN4295951 FACS ICC IF Alexa Fluor 488 Rat IgG2b kappa Log in to see 2-43
1 ABIN4295952 FACS ICC IF Alexa Fluor 647 Rat IgG2b kappa Log in to see 2-43

Top referenced anti-CD8 antibody for Immunocytochemistry

Similar anti-CD8 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 2 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Flow Cytometry (FACS) 128 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoaffinity Chromatography (IAC) 2 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1057), (550), (164), (77), (50), (41), (22), (22), (20), (20), (19), (16), (15), (13), (13), (12), (12), (12), (11), (11), (10), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1288), (522), (221), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(264), (220), (141), (81), (58), (46), (43), (39), (35), (31), (29), (28), (27), (26), (26), (26), (26), (25), (21), (21), (19), (17), (16), (15), (11), (10), (10), (10), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
(1651), (465), (446), (318), (270), (268), (267), (245), (231), (39), (38), (25), (24), (23), (18), (14), (11), (10), (7), (7), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4295972) Images
Purification Protein A or G purified
Immunogen Mouse thymus or spleen.
Clone 53-6-7
Isotype IgG2a kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN4295972) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Background Gene Symbol: CD8A
Molecular Weight Theoretical MW: 27 kDa
Gene ID 925
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN4295972) Images back to top
Application Notes Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Cell depletion, Inhibition of T Cell Function, CyTOF-readyEach lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 μg/10^6 cells in 100 μL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN4295972) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD8 Antibody (ABIN4295972)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Li, Wu, Tang, Li, Feng, Yao: "In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice." in: Cancer biotherapy & radiopharmaceuticals, Vol. 31, Issue 9, pp. 317-323, 2016 (Sample species: Human).

Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: "Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice." in: Neuropharmacology, Vol. 81, pp. 237-43, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kheradmand, Wang, Gibly, Zhang, Holland, Tasch, Graham, Kaufman, Miller, Shea, Luo: "Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions." in: Biomaterials, Vol. 32, Issue 20, pp. 4517-24, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Bankoti, Burnett, Navarro, Miller, Rase, Shepherd: "Effects of TCDD on the fate of naive dendritic cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 2, pp. 422-34, 2010

McLellan, Kapp, Eggert, Linden, Bommhardt, Bröcker, Kämmerer, Kämpgen: "Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression." in: Blood, Vol. 99, Issue 6, pp. 2084-93, 2002

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN4295972) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine s...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha e...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of C...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes ...
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blo...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocyt...
Immunocytochemistry (ICC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marr...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (ABIN4295972) Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen s...