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anti-Mouse (Murine) CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Epitope
C-Term
107
27
19
16
14
14
8
7
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
1069
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Rabbit
1303
519
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
264
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
1639
470
374
351
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189246
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
0.0009962841 ABIN1862305 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-247 Log in to see Polyclonal
0.0009962841 ABIN2749187 Func IHC (f) ICC FACS IP IHC (p) Rat IgG2a Log in to see 53-6-7 8
0.0009962841 ABIN4295939 FACS ICC IF Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN3200063 FACS Func ICC IHC IP PE Rat IgG2a Log in to see 53-6-7
0.0009962841 ABIN3200064 FACS Func ICC IHC IP PerCP Rat IgG2a Log in to see 53-6-7
0.0009962841 ABIN3200062 FACS Func ICC IHC IP FITC Rat IgG2a Log in to see 53-6-7
0.0009962841 ABIN3200061 FACS Func ICC IHC IP APC Rat IgG2a Log in to see 53-6-7
0.0009962841 ABIN4295950 FACS ICC IF Alexa Fluor 405 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295954 FACS ICC IF Allophycocyanin Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295955 FACS ICC IF Biotin Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295956 FACS ICC IF DyLight 650 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295957 FACS ICC IF FITC Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295958 FACS ICC IF DyLight 680 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295959 FACS ICC IF DyLight 488 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295962 FACS ICC IF PE Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295963 FACS ICC IF DyLight 550 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295965 FACS ICC IF DyLight 405 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295951 FACS ICC IF Alexa Fluor 488 Rat IgG2b kappa Log in to see 2-43
0.0009962841 ABIN4295952 FACS ICC IF Alexa Fluor 647 Rat IgG2b kappa Log in to see 2-43

Top referenced anti-CD8 antibody for Immunocytochemistry

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope C-Term
(107), (27), (19), (16), (14), (14), (8), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(1069), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1303), (519), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(264), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(1639), (470), (374), (351), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near the C-terminus of human CD8 alpha chain was used as an immunogen.
Clone EP1150Y
Isotype IgG

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as adisulfide-linked homodimer of two CD8alpha monomers, or a heterodimer of CD8alphaand CD8beta. These receptors interact with ligands, such as major histocompatibilitycomplex (MHC) class I, on the outside of the cell, with proteins inside the cell, such asthe tyrosine kinase p56lck, and possibly with proteins on the same cell-surface (1). CD8plays an important role in T cell development in the thymus and T cell activation in theperiphery (2). Alternate Names: anti-CD 8 antibody, anti-CD 8A antibody, anti-CD 8B antibody, anti-CD8 antigen alphapolypeptide antibody, anti-CD8A antibody, anti-CD8a antigen antibody, anti-CD8amolecule antibody, anti-CD8B antibody, anti-CD8b antigen antibody, anti-CD8b antigenisoform 2 antibody, anti-CD8b molecule antibody, anti-CD8B protein antibody,anti-CD8B1 antibody, anti-Leu2 antibody, anti-Leu2 T lymphocyte antigen antibody,anti-Ly3 antibody, anti-LYT3 antibody, anti-MAL antibody, anti-MGC119115 antibody,anti-OKT8 T Cell antigen antibody, anti-p32 antibody, anti-T cell antigen Leu2 antibody,anti-T cell surface glycoprotein CD8 alpha chain antibody, anti-T cell surfaceglycoprotein CD8 beta chain antibody, anti-T lymphocyte differentiation antigen T8/Leu2 antibody.
Gene Symbol: CD8A
Gene ID 925, 12525, 24930
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes For Mouse, this antibody is only recommended for Western blot,flow cytometry, this antibody is not useful for IHC on mouse tissue. Rat cross reactivity tested by Western blot only. Customer review indicates poor reactivity in Woodchuck.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-500, Western Blot 1:1000-10000
Protocol Protocol specific for CD8 alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD8 antibody (CD8a Molecule) (C-Term) (ABIN189246) Western blot analysis on THP-1 lysate using anti-CD8 RabMAb (ABIN189246), dilution 1:...
Immunohistochemistry (IHC) image for anti-CD8 antibody (CD8a Molecule) (C-Term) (ABIN189246) Immunohistochemical staining of paraffin-embedded human tissues using anti-CD8 RabMAb