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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||4 Antibodies|
|Blocking Reagent (BR)||9 Antibodies|
|Cytotoxicity Test (CyTox)||9 Antibodies|
|Flow Cytometry (FACS)||345 Antibodies|
|Functional Studies (Func)||23 Antibodies|
|Immunocytochemistry (ICC)||27 Antibodies|
|Immunofluorescence (IF)||40 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||72 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||11 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||5 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||52 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||4 Antibodies|
|Immunoprecipitation (IP)||62 Antibodies|
|Intracellular Flow Cytometry (ICFC)||7 Antibodies|
|Separation (Sep)||2 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
|21 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN1177008) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||Mouse thymus / spleen Cells|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN1177008) Images back to top
|Alternative Name||CD8a (CD8A Antibody Abstract)|
The 53-6.7 antibody has been reported to react with the 38 kDa alpha and 34 kDa alpha' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 alpha and alpha' chains (CD8a) form heterodimers with the CD8 beta chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 alphabeta heterodimer (the alpha' chain is absent). Subsets of gammadelta TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 alpha and alpha' chains arise from alternatively spliced messengers of a single CD8a gene. The longer alpha form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated alpha' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 alpha- and alpha'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis.
Synonyms: Ly-2, Lyt-2
|Research Area||Major Histocompatibility Complex (MHC)|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN1177008) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN1177008) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-CD8 Antibody (ABIN1177008)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
|Product cited in:||
Leishman, Naidenko, Attinger et al.: "T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL." in: Science (New York, N.Y.), Vol. 294, Issue 5548, pp. 1936-9, 2001 (PubMed).
Traver, Akashi, Manz et al.: "Development of CD8alpha-positive dendritic cells from a common myeloid progenitor." in: Science (New York, N.Y.), Vol. 290, Issue 5499, pp. 2152-4, 2000 (PubMed).
Mitnacht, Bischof, Torres-Nagel et al.: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).
Negm, Mansour, Saad et al.: "Structural characterization of an Lyt-2/3 homolog expressed on Bufo regularis lymphocytes." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 113, Issue 1, pp. 79-87, 1997 (PubMed).
Anel, ORourke, Kleinfeld et al.: "T cell receptor and CD8-dependent tyrosine phosphorylation events in cytotoxic T lymphocytes: activation of p56lck by CD8 binding to class I protein." in: European journal of immunology, Vol. 26, Issue 10, pp. 2310-9, 1996 (PubMed).
Alexander-Miller, Leggatt, Sarin et al.: "Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 485-92, 1996 (PubMed).
Sydora, Brossay, Hagenbaugh et al.: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, 1996 (PubMed).
Fujiura, Kawaguchi, Kondo et al.: "Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, 1996 (PubMed).
ORourke, Mescher: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, 1993 (PubMed).
Wang, Klein: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, 1994 (PubMed).
Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995 (PubMed).
van Ewijk, van Soest, van den Engh: "Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2594-604, 1982 (PubMed).
Walker, Murray, Hogarth et al.: "Comparison of thymic and peripheral T cell Ly-2/3 antigens." in: European journal of immunology, Vol. 14, Issue 10, pp. 906-10, 1984 (PubMed).
Ledbetter, Seaman, Tsu et al.: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, 1981 (PubMed).
Zamoyska, Vollmer, Sizer et al.: "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA." in: Cell, Vol. 43, Issue 1, pp. 153-63, 1986 (PubMed).
Zamoyska, Derham, Gorman et al.: "Inability of CD8 alpha' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo." in: Nature, Vol. 342, Issue 6247, pp. 278-81, 1989 (PubMed).
MacDonald, Schreyer, Howe et al.: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, 1990 (PubMed).
Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992 (PubMed).
Murosaki, Yoshikai, Ishida et al.: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, 1992 (PubMed).
Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).
Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).