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anti-Mouse (Murine) CD8 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1059
531
167
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Rat
1286
511
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
258
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1617
451
356
343
285
282
255
238
214
37
33
25
23
17
14
10
7
6
6
6
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN114166
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008837687 ABIN118177 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
0.0008837687 ABIN118133 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 14
0.0008837687 ABIN114167 FACS IHC (fro) Rat IgG2b Log in to see YTS169-4 6
0.0008837687 ABIN951388 IHC (fro) FACS FITC Rat IgG2b Log in to see YTS169-4 4
0.0008837687 ABIN356190 FACS IHC (fro) IP FITC Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN319545 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 1
0.0008837687 ABIN319569 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
0.0008837687 ABIN319570 FACS IHC (fro) Rat IgG2a Log in to see YTS105-18 4
0.0008837687 ABIN118132 FACS IHC (fro) IF Rat IgG2a Log in to see KT15 2
0.0008837687 ABIN114175 FACS IHC (fro) Rat IgG2a Log in to see CT-CD8a 2
0.0008837687 ABIN317092 FACS IHC (fro) IHC (p) IP Rat IgG2a Log in to see 07-06-53 2
0.0008837687 ABIN356185 FACS IHC (fro) IP APC-Cy7 Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN356186 FACS IHC (fro) IP APC Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN356196 FACS IHC (fro) IP PE Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN356194 FACS IHC (fro) IP PE-Cy7 Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN356192 Func FACS IHC (fro) IP Rat IgG2a Log in to see 07-06-53 7
0.0008837687 ABIN1177008 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53-6-7 25
0.0008837687 ABIN262311 FACS IHC (fro) IHC Rat IgG2b lambda Log in to see 5H10-1
0.0008837687 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18
0.0008837687 ABIN255912 FACS IHC (fro) IHC Rat IgG2b Log in to see YTS169-4

Top referenced anti-CD8 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 470 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 138 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies
Intracellular Flow Cytometry (ICFC) 7 Antibodies
Separation (Sep) 2 Antibodies
Western Blotting (WB) 20 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1059), (531), (167), (68), (47), (40), (25), (22), (20), (20), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(1286), (511), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(258), (217), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1617), (451), (356), (343), (285), (282), (255), (238), (214), (37), (33), (25), (23), (17), (14), (10), (7), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114166) Images
Specificity Antibody CL007 reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/ suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology.
Purification Protein G Chromatography
Immunogen Murine thymocytes
Clone YTS169-4
Isotype IgG2b

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN114166) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN114166) Images back to top
Application Notes Flow Cytometry. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50µl of thissuspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 0. 1-0. 5 µg* of ABIN114165 per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN114166) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD8 Antibody (ABIN114166)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mueller, Davies, Krahl, Sarvetnick: "IL-4 expression by grafts from transgenic mice fails to prevent allograft rejection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1599-603, 1997

Stevenson, Freeman, Bangham, Hawke: "Virus dissemination through the brain parenchyma without immunologic control." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 4, pp. 1876-84, 1997

Cobbold, Jayasuriya, Nash, Prospero, Waldmann: "Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo." in: Nature, Vol. 312, Issue 5994, pp. 548-51, 1985

Galfrè, Milstein, Wright: "Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG." in: Nature, Vol. 277, Issue 5692, pp. 131-3, 1979

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN114166) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN114166) Tissue Distribution by Flow Cytometry Analysis: