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anti-Mouse (Murine) CD8 antibody for Immunohistochemistry (Zinc-fixed Sections)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1059
531
167
68
47
40
25
22
20
20
19
16
15
13
12
12
11
11
10
10
8
8
5
3
3
2
2
2
2
1
1
1
Host
Rat
1286
511
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
259
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
1617
451
356
342
285
282
255
238
214
36
33
25
23
17
13
10
7
6
5
5
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1177008
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008700774 ABIN2689434 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see 53 9
0.0008700774 ABIN2689432 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53 24
0.0008700774 ABIN2689433 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53

Top referenced anti-CD8 antibody for Immunohistochemistry (Zinc-fixed Sections)

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 470 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 138 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1059), (531), (167), (68), (47), (40), (25), (22), (20), (20), (19), (16), (15), (13), (12), (12), (11), (11), (10), (10), (8), (8), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(1286), (511), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(259), (217), (142), (86), (52), (42), (40), (39), (30), (30), (29), (28), (27), (26), (25), (25), (25), (25), (25), (21), (21), (18), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
(1617), (451), (356), (342), (285), (282), (255), (238), (214), (36), (33), (25), (23), (17), (13), (10), (7), (6), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN1177008) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse thymus / spleen Cells
Clone 53-6-7
Isotype IgG2a, kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN1177008) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background The 53-6.7 antibody has been reported to react with the 38 kDa alpha and 34 kDa alpha' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 alpha and alpha' chains (CD8a) form heterodimers with the CD8 beta chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 alphabeta heterodimer (the alpha' chain is absent). Subsets of gammadelta TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 alpha and alpha' chains arise from alternatively spliced messengers of a single CD8a gene. The longer alpha form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated alpha' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 alpha- and alpha'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis.
Synonyms: Ly-2, Lyt-2
Research Area Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN1177008) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN1177008) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD8 Antibody (ABIN1177008)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Leishman, Naidenko, Attinger, Koning, Lena, Xiong, Chang, Reinherz, Kronenberg, Cheroutre: "T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL." in: Science (New York, N.Y.), Vol. 294, Issue 5548, pp. 1936-9, 2001

Traver, Akashi, Manz, Merad, Miyamoto, Engleman, Weissman: "Development of CD8alpha-positive dendritic cells from a common myeloid progenitor." in: Science (New York, N.Y.), Vol. 290, Issue 5499, pp. 2152-4, 2000

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Negm, Mansour, Saad, Abdel Halim: "Structural characterization of an Lyt-2/3 homolog expressed on Bufo regularis lymphocytes." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 113, Issue 1, pp. 79-87, 1997

Anel, ORourke, Kleinfeld, Mescher: "T cell receptor and CD8-dependent tyrosine phosphorylation events in cytotoxic T lymphocytes: activation of p56lck by CD8 binding to class I protein." in: European journal of immunology, Vol. 26, Issue 10, pp. 2310-9, 1996

Alexander-Miller, Leggatt, Sarin, Berzofsky: "Role of antigen, CD8, and cytotoxic T lymphocyte (CTL) avidity in high dose antigen induction of apoptosis of effector CTL." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 485-92, 1996

Fujiura, Kawaguchi, Kondo, Obana, Yamamoto, Nanno, Ishikawa: "Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, 1996

Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, 1996

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995

Nakayama, Nakayama, Negishi, Kuida, Louie, Kanagawa, Nakauchi, Loh: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, 1994

Wang, Klein: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, 1994

ORourke, Mescher: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, 1993

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992

Murosaki, Yoshikai, Ishida, Nakamura, Matsuzaki, Takimoto, Yuuki, Nomoto: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, 1992

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

MacDonald, Schreyer, Howe, Bron: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, 1990

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989

Zamoyska, Derham, Gorman, von Hoegen, Bolen, Veillette, Parnes: "Inability of CD8 alpha' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo." in: Nature, Vol. 342, Issue 6247, pp. 278-81, 1989

Zamoyska, Vollmer, Sizer, Liaw, Parnes: "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA." in: Cell, Vol. 43, Issue 1, pp. 153-63, 1986