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anti-Mouse (Murine) CD8 antibody for Immunoprecipitation

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Mouse (Murine)
1069
537
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Rat
1303
518
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to FITC
263
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
18
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
1638
469
374
350
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
6
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN356190
$ 176.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN317092 FACS IHC (fro) IHC (p) IP Rat IgG2a Log in to see 07-06-53 2
0.00089556724 ABIN2658875 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 10
0.00089556724 ABIN2660735 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 10
0.00089556724 ABIN2657489 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
0.00089556724 ABIN289074 FACS IP IHC Biotin Rat IgG2a Log in to see 53-6-7
0.00089556724 ABIN356185 FACS IHC (fro) IP APC-Cy7 Rat IgG2a Log in to see 07-06-53 7
0.00089556724 ABIN356186 FACS IHC (fro) IP APC Rat IgG2a Log in to see 07-06-53 7
0.00089556724 ABIN356196 FACS IHC (fro) IP PE Rat IgG2a Log in to see 07-06-53 7
0.00089556724 ABIN356194 FACS IHC (fro) IP PE-Cy7 Rat IgG2a Log in to see 07-06-53 7
0.00089556724 ABIN284334 FACS IP IHC Rat IgG2a Log in to see 53-6-7
0.00089556724 ABIN356192 Func FACS IHC (fro) IP Rat IgG2a Log in to see 07-06-53 7
0.00089556724 ABIN1177008 BR Func IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53-6-7 25
0.00089556724 ABIN2658876 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 11
0.00089556724 ABIN2660736 BR FACS IP APC-Cy7 Rat IgG2a kappa Log in to see 53-6-7 12
0.00089556724 ABIN2657490 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
0.00089556724 ABIN2689432 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see 53 24
0.00089556724 ABIN356187 FACS IHC (fro) IP Biotin Rat IgG2a Log in to see 07-06-53
0.00089556724 ABIN153109 FACS IHC (fro) IP IHC Rat IgG1 kappa Log in to see IBL-3-25
0.00089556724 ABIN356197 FACS IHC (fro) IP PE-TR Rat IgG2a Log in to see 07-06-53
0.00089556724 ABIN2749187 Func IHC (f) ICC FACS IP IHC (p) Rat IgG2a Log in to see 53-6-7 8

Top referenced anti-CD8 antibody for Immunoprecipitation

Similar anti-CD8 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 4 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cytotoxicity Test (CyTox) 9 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 469 Antibodies
Functional Studies (Func) 23 Antibodies
Immunocytochemistry (ICC) 75 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 137 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 4 Antibodies
Immunoprecipitation (IP) 64 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(1069), (537), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rat
(1303), (518), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to FITC
(263), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (18), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(1638), (469), (374), (350), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN356190) Images
Specificity This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Characteristics Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
Clone 07-06-53
Isotype IgG2a

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN356190) Images back to top
Antigen
Alternative Name CD8 (CD8A Antibody Abstract)
Background In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN356190) Images back to top
Application Notes Flow Cytometry: < / = 2 μg/10^6 cells. (Ref.8,9)Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.10)Immunoprecipitation. (Ref.8)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN356190) Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD8 Antibody (ABIN356190)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991

Swain, Dialynas, Fitch, English: "Monoclonal antibody to L3T4 blocks the function of T cells specific for class 2 major histocompatibility complex antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 3, pp. 1118-23, 1984

Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN356190) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN356190) anti-CD8a Molecule (CD8A) antibody (FITC)