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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||4 Antibodies|
|Blocking Reagent (BR)||9 Antibodies|
|Cytotoxicity Test (CyTox)||9 Antibodies|
|Flow Cytometry (FACS)||345 Antibodies|
|Functional Studies (Func)||23 Antibodies|
|Immunocytochemistry (ICC)||27 Antibodies|
|Immunofluorescence (IF)||40 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||72 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||11 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||5 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||52 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||4 Antibodies|
|Immunoprecipitation (IP)||62 Antibodies|
|Intracellular Flow Cytometry (ICFC)||7 Antibodies|
|Separation (Sep)||2 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD8 antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|7 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN356190) Images
|Specificity||This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)|
|Characteristics||Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN356190) Images back to top
|Alternative Name||CD8 (CD8A Antibody Abstract)|
|Background||In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN356190) Images back to top
Flow Cytometry: < / = 2 μg/10^6 cells. (Ref.8,9)Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.10)Immunoprecipitation. (Ref.8)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN356190) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD8 Antibody (ABIN356190)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983 (PubMed).
Swain, Dialynas, Fitch et al.: "Monoclonal antibody to L3T4 blocks the function of T cells specific for class 2 major histocompatibility complex antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 3, pp. 1118-23, 1984 (PubMed).
Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).
Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992 (PubMed).
Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991 (PubMed).
Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).
Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN356190) back to top